Biochemical and molecular stress responses to enrofloxacin in Mytilus galloprovincialis: transient or lasting effects?
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| Title: | Biochemical and molecular stress responses to enrofloxacin in Mytilus galloprovincialis: transient or lasting effects? |
|---|---|
| Authors: | Giannessi, J.1 (AUTHOR), Pretti, C.1,2 (AUTHOR) carlo.pretti@unipi.it, Meucci, V.1 (AUTHOR), Intorre, L.1 (AUTHOR), De Marchi, L.1 (AUTHOR), Gabbrielli, B.1 (AUTHOR), Baratti, M.3 (AUTHOR) |
| Source: | Aquatic Toxicology. Jan2026, Vol. 290, pN.PAG-N.PAG. 1p. |
| Subjects: | Mytilus galloprovincialis, Energy metabolism, Fluoroquinolones, Physiological stress, Bacterial contamination, Chemical reactions, Marine habitats, Metabolic detoxification |
| Abstract: | • Detoxification genes (abcb, cyp4y1) are upregulated at early exposure stages. • ENR affects energy metabolism and reduces cellular energy reserves. • GST activity increases over time, indicating phase II detox activation. • Alterations persist after exposure, suggesting incomplete recovery. Antibiotic contamination is an emerging threat in marine environments due to its potential to alter key physiological processes in non-target organisms. Among veterinary antibiotics, enrofloxacin (ENR), a widely used fluoroquinolone, is frequently detected in coastal waters. This study investigated the biological effects of ENR exposure in the Mediterranean mussel Mytilus galloprovincialis using an integrated approach combining gene expression, biochemical, and bioenergetic endpoints. Mussels were exposed to two environmentally relevant concentrations of ENR (5 and 500 ng/L) for 7 days (EXP7) and 14 days (EXP14), followed by a 14-day recovery period (REC). Gill tissues were analyzed for transcriptional changes in genes related to detoxification, oxidative stress, apoptosis, and energy metabolism. In addition, energy reserves, mitochondrial activity, and indicators of genotoxicity and antioxidant responses were measured. Results showed that ENR exposure modulated the expression of several genes, including early upregulation of abcb and cyp4y1 , and downregulation of pkpyr and icdh , indicating activation of detoxification pathways and energy metabolism shifts. Biochemical analyses revealed increased mitochondrial activity (ETS), mobilization of energy reserves, and sustained elevation of GST activity, while DNA strand break levels confirmed a potential genotoxic effect. Despite the removal of the contaminant, most stress responses did not fully revert during recovery. These findings demonstrate that even low concentrations of ENR can disrupt mussel physiology at multiple levels, with effects persisting beyond exposure. The study underscores the importance of incorporating sub-lethal biomarkers into environmental risk assessments and supports the inclusion of veterinary antibiotics like ENR in marine monitoring programs to protect coastal ecosystem health. [ABSTRACT FROM AUTHOR] |
| Copyright of Aquatic Toxicology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 189851042 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Biochemical and molecular stress responses to enrofloxacin in Mytilus galloprovincialis: transient or lasting effects? – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Giannessi%2C+J%2E%22">Giannessi, J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pretti%2C+C%2E%22">Pretti, C.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> carlo.pretti@unipi.it</i><br /><searchLink fieldCode="AR" term="%22Meucci%2C+V%2E%22">Meucci, V.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Intorre%2C+L%2E%22">Intorre, L.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22De+Marchi%2C+L%2E%22">De Marchi, L.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gabbrielli%2C+B%2E%22">Gabbrielli, B.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Baratti%2C+M%2E%22">Baratti, M.</searchLink><relatesTo>3</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Aquatic+Toxicology%22">Aquatic Toxicology</searchLink>. Jan2026, Vol. 290, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Mytilus+galloprovincialis%22">Mytilus galloprovincialis</searchLink><br /><searchLink fieldCode="DE" term="%22Energy+metabolism%22">Energy metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Fluoroquinolones%22">Fluoroquinolones</searchLink><br /><searchLink fieldCode="DE" term="%22Physiological+stress%22">Physiological stress</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+contamination%22">Bacterial contamination</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+reactions%22">Chemical reactions</searchLink><br /><searchLink fieldCode="DE" term="%22Marine+habitats%22">Marine habitats</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolic+detoxification%22">Metabolic detoxification</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: • Detoxification genes (abcb, cyp4y1) are upregulated at early exposure stages. • ENR affects energy metabolism and reduces cellular energy reserves. • GST activity increases over time, indicating phase II detox activation. • Alterations persist after exposure, suggesting incomplete recovery. Antibiotic contamination is an emerging threat in marine environments due to its potential to alter key physiological processes in non-target organisms. Among veterinary antibiotics, enrofloxacin (ENR), a widely used fluoroquinolone, is frequently detected in coastal waters. This study investigated the biological effects of ENR exposure in the Mediterranean mussel Mytilus galloprovincialis using an integrated approach combining gene expression, biochemical, and bioenergetic endpoints. Mussels were exposed to two environmentally relevant concentrations of ENR (5 and 500 ng/L) for 7 days (EXP7) and 14 days (EXP14), followed by a 14-day recovery period (REC). Gill tissues were analyzed for transcriptional changes in genes related to detoxification, oxidative stress, apoptosis, and energy metabolism. In addition, energy reserves, mitochondrial activity, and indicators of genotoxicity and antioxidant responses were measured. Results showed that ENR exposure modulated the expression of several genes, including early upregulation of abcb and cyp4y1 , and downregulation of pkpyr and icdh , indicating activation of detoxification pathways and energy metabolism shifts. Biochemical analyses revealed increased mitochondrial activity (ETS), mobilization of energy reserves, and sustained elevation of GST activity, while DNA strand break levels confirmed a potential genotoxic effect. Despite the removal of the contaminant, most stress responses did not fully revert during recovery. These findings demonstrate that even low concentrations of ENR can disrupt mussel physiology at multiple levels, with effects persisting beyond exposure. The study underscores the importance of incorporating sub-lethal biomarkers into environmental risk assessments and supports the inclusion of veterinary antibiotics like ENR in marine monitoring programs to protect coastal ecosystem health. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Aquatic Toxicology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.aquatox.2025.107626 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Mytilus galloprovincialis Type: general – SubjectFull: Energy metabolism Type: general – SubjectFull: Fluoroquinolones Type: general – SubjectFull: Physiological stress Type: general – SubjectFull: Bacterial contamination Type: general – SubjectFull: Chemical reactions Type: general – SubjectFull: Marine habitats Type: general – SubjectFull: Metabolic detoxification Type: general Titles: – TitleFull: Biochemical and molecular stress responses to enrofloxacin in Mytilus galloprovincialis: transient or lasting effects? Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Giannessi, J. – PersonEntity: Name: NameFull: Pretti, C. – PersonEntity: Name: NameFull: Meucci, V. – PersonEntity: Name: NameFull: Intorre, L. – PersonEntity: Name: NameFull: De Marchi, L. – PersonEntity: Name: NameFull: Gabbrielli, B. – PersonEntity: Name: NameFull: Baratti, M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 0166445X Numbering: – Type: volume Value: 290 Titles: – TitleFull: Aquatic Toxicology Type: main |
| ResultId | 1 |
