Effective role of putrescene in mitigating harmful effects of salinity on rapeseed seedlings via modulating morpho-physiochemical and molecular processes.
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| Title: | Effective role of putrescene in mitigating harmful effects of salinity on rapeseed seedlings via modulating morpho-physiochemical and molecular processes. |
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| Authors: | El-Badri, Ali Mahmoud1,2 (AUTHOR), Batool, Maria1 (AUTHOR), Mohamed, Ibrahim A.A.1,3 (AUTHOR), Hashem, Ahmed M.4 (AUTHOR), Wang, Zongkai1 (AUTHOR), Wang, Chunyun1 (AUTHOR), Maaz-Ullah1 (AUTHOR), Ikram, Muhammad1,5 (AUTHOR), Shalby, Nesma3 (AUTHOR), Khatab, Ahmed2 (AUTHOR), Sherif, Ahmed2 (AUTHOR), Freeg, Haytham A.2 (AUTHOR), Yang, Baizhan6 (AUTHOR), Bai, Fei7 (AUTHOR), Zou, Jun1 (AUTHOR), Wang, Bo1 (AUTHOR) wangbo@mail.hzau.edu.cn, Zhou, Guangsheng1 (AUTHOR) zhougs@mail.hzau.edu.cn |
| Source: | Industrial Crops & Products. Dec2025, Vol. 238, pN.PAG-N.PAG. 1p. |
| Subjects: | Putrescine, Rapeseed, Ionic equilibrium, Antioxidants, Salinization, Physiology, Germination, Oxidative stress |
| Abstract: | Understanding tolerance mechanisms and categorizing reliable physiochemical indicators related to their mechanisms is important for enhancing rapeseed tolerance and sustainable agricultural development. Putrescine (Put), one of the important polyamines, plays a promising role in promoting the synthesis of bioactive compounds related to plant development, especially under stress conditions. Herein, the potential role of Put in alleviating salt stress in rapeseed has been thoroughly investigated via priming application with different concentrations (0.1, 0.3, 0.5, 0.7, and 1 mM). Seed germination-related parameters and hormone-related genes were studied during seed imbibition under 150 mM of NaCl. Additionally, morpho-physiochemical characteristics, ionic content, and ion exchange-related genes were investigated in the shoots and roots of 7-day-old seedlings under salinity stress. Our results showed that Put-priming improved germination parameters by regulating seed germination and hormone-related genes under salinity stress, especially at 0.3 and 0.5 mM Put, compared with unprimed and hydroprimed (HP) seeds. Besides, it significantly enhanced seedling growth by preventing the degradation of photosynthetic pigments and modulating the proline and soluble solute accumulation in shoots and roots. Moreover, MDA, H 2 O 2 , and O 2 contents decreased with Put-priming, especially at 0.5 mM, indicating the significant role of Put in ROS scavenging and inhibiting lipid peroxidation upon stress. Put-priming alleviated salt-induced oxidative stress by enhancing the structure and function of the enzymatic and non-enzymatic antioxidative systems, which protect the integrity of cellular membranes in seedlings under stress conditions. Our study observed increased Na+ and decreased K+ contents under salt treatments versus control (Ctrl), whereas Put treatments not only reduced Na+ but also increased K+ accumulation, lowering the Na+/K+ ratio, especially at 0.3 and 0.5 mM Put versus unprimed and HP seedlings. Put-priming showed strikingly stronger effects on intracellular Na+ and K+ homeostasis by regulating ion exchange-related genes, which upregulated the gene expression of BnH + -ATPase , BnNHX6 , BnSOS1 , BnSKOR , BnHKT1, and BnHKT8 that modulated Na+ influx and K+ efflux under salinity stress. Our results provide an integrative understanding and scientific basis for the exogenous application of Put as a promising method for improving salt tolerance in rapeseed seedlings at morpho-physiochemical and molecular levels, which provides a convenient, fast, and environmentally friendly strategy to help crops adapt to be used for cultivation in saline soil. [Display omitted] • Put regulating germination and hormone-related genes upon salt stress conditions. • Put enhances seedling growth, preserves photosynthetic pigments, and reduces oxidative stress. • Put-priming reduced salt-induced ion toxicity via osmolyte and antioxidant systems. • Put-priming modulates intracellular Na+ and K+ homeostasis by upregulating genes of ion exchange. • Put is a promising material in ameliorating rapeseed growth under salt stress. [ABSTRACT FROM AUTHOR] |
| Copyright of Industrial Crops & Products is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 189905229 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Effective role of putrescene in mitigating harmful effects of salinity on rapeseed seedlings via modulating morpho-physiochemical and molecular processes. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22El-Badri%2C+Ali+Mahmoud%22">El-Badri, Ali Mahmoud</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Batool%2C+Maria%22">Batool, Maria</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mohamed%2C+Ibrahim+A%2EA%2E%22">Mohamed, Ibrahim A.A.</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hashem%2C+Ahmed+M%2E%22">Hashem, Ahmed M.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zongkai%22">Wang, Zongkai</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Chunyun%22">Wang, Chunyun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Maaz-Ullah%22">Maaz-Ullah</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ikram%2C+Muhammad%22">Ikram, Muhammad</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shalby%2C+Nesma%22">Shalby, Nesma</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Khatab%2C+Ahmed%22">Khatab, Ahmed</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sherif%2C+Ahmed%22">Sherif, Ahmed</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Freeg%2C+Haytham+A%2E%22">Freeg, Haytham A.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Baizhan%22">Yang, Baizhan</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bai%2C+Fei%22">Bai, Fei</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zou%2C+Jun%22">Zou, Jun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Bo%22">Wang, Bo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wangbo@mail.hzau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Guangsheng%22">Zhou, Guangsheng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> zhougs@mail.hzau.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Industrial+Crops+%26+Products%22">Industrial Crops & Products</searchLink>. Dec2025, Vol. 238, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Putrescine%22">Putrescine</searchLink><br /><searchLink fieldCode="DE" term="%22Rapeseed%22">Rapeseed</searchLink><br /><searchLink fieldCode="DE" term="%22Ionic+equilibrium%22">Ionic equilibrium</searchLink><br /><searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Salinization%22">Salinization</searchLink><br /><searchLink fieldCode="DE" term="%22Physiology%22">Physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Germination%22">Germination</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidative+stress%22">Oxidative stress</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Understanding tolerance mechanisms and categorizing reliable physiochemical indicators related to their mechanisms is important for enhancing rapeseed tolerance and sustainable agricultural development. Putrescine (Put), one of the important polyamines, plays a promising role in promoting the synthesis of bioactive compounds related to plant development, especially under stress conditions. Herein, the potential role of Put in alleviating salt stress in rapeseed has been thoroughly investigated via priming application with different concentrations (0.1, 0.3, 0.5, 0.7, and 1 mM). Seed germination-related parameters and hormone-related genes were studied during seed imbibition under 150 mM of NaCl. Additionally, morpho-physiochemical characteristics, ionic content, and ion exchange-related genes were investigated in the shoots and roots of 7-day-old seedlings under salinity stress. Our results showed that Put-priming improved germination parameters by regulating seed germination and hormone-related genes under salinity stress, especially at 0.3 and 0.5 mM Put, compared with unprimed and hydroprimed (HP) seeds. Besides, it significantly enhanced seedling growth by preventing the degradation of photosynthetic pigments and modulating the proline and soluble solute accumulation in shoots and roots. Moreover, MDA, H 2 O 2 , and O 2 contents decreased with Put-priming, especially at 0.5 mM, indicating the significant role of Put in ROS scavenging and inhibiting lipid peroxidation upon stress. Put-priming alleviated salt-induced oxidative stress by enhancing the structure and function of the enzymatic and non-enzymatic antioxidative systems, which protect the integrity of cellular membranes in seedlings under stress conditions. Our study observed increased Na+ and decreased K+ contents under salt treatments versus control (Ctrl), whereas Put treatments not only reduced Na+ but also increased K+ accumulation, lowering the Na+/K+ ratio, especially at 0.3 and 0.5 mM Put versus unprimed and HP seedlings. Put-priming showed strikingly stronger effects on intracellular Na+ and K+ homeostasis by regulating ion exchange-related genes, which upregulated the gene expression of BnH + -ATPase , BnNHX6 , BnSOS1 , BnSKOR , BnHKT1, and BnHKT8 that modulated Na+ influx and K+ efflux under salinity stress. Our results provide an integrative understanding and scientific basis for the exogenous application of Put as a promising method for improving salt tolerance in rapeseed seedlings at morpho-physiochemical and molecular levels, which provides a convenient, fast, and environmentally friendly strategy to help crops adapt to be used for cultivation in saline soil. [Display omitted] • Put regulating germination and hormone-related genes upon salt stress conditions. • Put enhances seedling growth, preserves photosynthetic pigments, and reduces oxidative stress. • Put-priming reduced salt-induced ion toxicity via osmolyte and antioxidant systems. • Put-priming modulates intracellular Na+ and K+ homeostasis by upregulating genes of ion exchange. • Put is a promising material in ameliorating rapeseed growth under salt stress. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Industrial Crops & Products is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.indcrop.2025.122331 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Putrescine Type: general – SubjectFull: Rapeseed Type: general – SubjectFull: Ionic equilibrium Type: general – SubjectFull: Antioxidants Type: general – SubjectFull: Salinization Type: general – SubjectFull: Physiology Type: general – SubjectFull: Germination Type: general – SubjectFull: Oxidative stress Type: general Titles: – TitleFull: Effective role of putrescene in mitigating harmful effects of salinity on rapeseed seedlings via modulating morpho-physiochemical and molecular processes. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: El-Badri, Ali Mahmoud – PersonEntity: Name: NameFull: Batool, Maria – PersonEntity: Name: NameFull: Mohamed, Ibrahim A.A. – PersonEntity: Name: NameFull: Hashem, Ahmed M. – PersonEntity: Name: NameFull: Wang, Zongkai – PersonEntity: Name: NameFull: Wang, Chunyun – PersonEntity: Name: NameFull: Maaz-Ullah – PersonEntity: Name: NameFull: Ikram, Muhammad – PersonEntity: Name: NameFull: Shalby, Nesma – PersonEntity: Name: NameFull: Khatab, Ahmed – PersonEntity: Name: NameFull: Sherif, Ahmed – PersonEntity: Name: NameFull: Freeg, Haytham A. – PersonEntity: Name: NameFull: Yang, Baizhan – PersonEntity: Name: NameFull: Bai, Fei – PersonEntity: Name: NameFull: Zou, Jun – PersonEntity: Name: NameFull: Wang, Bo – PersonEntity: Name: NameFull: Zhou, Guangsheng IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 12 Text: Dec2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 09266690 Numbering: – Type: volume Value: 238 Titles: – TitleFull: Industrial Crops & Products Type: main |
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