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Enhanced DNA Extraction From Dimorphic Fungi: Exploring the Advantages of Blastospores Versus Mycelia.

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Title: Enhanced DNA Extraction From Dimorphic Fungi: Exploring the Advantages of Blastospores Versus Mycelia.
Authors: El Khatib, Ahmad1 (AUTHOR), Sabbagh, Dalal1 (AUTHOR), Dimassi, Sarah1 (AUTHOR), Akl, Hadi1 (AUTHOR), El Khoury, Karen1 (AUTHOR), Saba, Jimmy Bou1 (AUTHOR), Al Kazzi, Mariline1 (AUTHOR), Dimassi, Hani2 (AUTHOR), Tokajian, Sima3 (AUTHOR), Nemer, Nabil4 (AUTHOR), Al Khoury, Charbel1 (AUTHOR) Charbel.alkhoury@lau.edu.lb
Source: Biotechnology & Applied Biochemistry. Dec2025, Vol. 72 Issue 6, p1788-1797. 10p.
Subjects: Fungal spores, Fungi, Molecular weights, Extraction (Chemistry), Nucleic acid isolation methods, Agonomycetales, Statistical reliability, Polymerase chain reaction
Abstract: The extraction of high‐quality DNA from fungi remains a significant challenge due to the structural complexity and resilience of fungal cell walls. This study presents the first comprehensive comparison of DNA extraction efficiency between blastospores (BS) and mycelial cells (MCs) in dimorphic fungi. We evaluated six DNA extraction procedures and two pre‐treatment methods to determine their effectiveness in extracting DNA of high quality and yield from both forms. DNA quantification was performed using absolute RT‐qPCR targeting the single‐copy γ‐Actin gene, with specificity and efficiency validated through standardized protocols. Our results showed that BS consistently yielded higher DNA quantity and quality across all conditions, with a mean antilog DNA copy concentration of 5.03 ± 0.20, compared to 2.51 ± 0.20 for MC. DNA quality was superior in BS, as indicated by significantly better 260/280 and 260/230 ratios (31.5% vs. 7.4% for MC, p value = 0.002). Additionally, BS produced higher molecular weight DNA (10,770.5 base pairs vs. 8139.0 base pairs for MC, p value < 0.001) and exhibited greater long‐term stability at both 4°C and −20°C. The results demonstrate that BS serve as a more reliable source of high‐quality DNA compared to MC. This finding enhances current methodologies in molecular biology and supports more accurate analyses, particularly in studies involving dimorphic fungi. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Enhanced DNA Extraction From Dimorphic Fungi: Exploring the Advantages of Blastospores Versus Mycelia.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22El+Khatib%2C+Ahmad%22&quot;&gt;El Khatib, Ahmad&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Sabbagh%2C+Dalal%22&quot;&gt;Sabbagh, Dalal&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Dimassi%2C+Sarah%22&quot;&gt;Dimassi, Sarah&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Akl%2C+Hadi%22&quot;&gt;Akl, Hadi&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22El+Khoury%2C+Karen%22&quot;&gt;El Khoury, Karen&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Saba%2C+Jimmy+Bou%22&quot;&gt;Saba, Jimmy Bou&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Al+Kazzi%2C+Mariline%22&quot;&gt;Al Kazzi, Mariline&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Dimassi%2C+Hani%22&quot;&gt;Dimassi, Hani&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Tokajian%2C+Sima%22&quot;&gt;Tokajian, Sima&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Nemer%2C+Nabil%22&quot;&gt;Nemer, Nabil&lt;/searchLink&gt;&lt;relatesTo&gt;4&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Al+Khoury%2C+Charbel%22&quot;&gt;Al Khoury, Charbel&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;i&gt; Charbel.alkhoury@lau.edu.lb&lt;/i&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Biotechnology+%26+Applied+Biochemistry%22&quot;&gt;Biotechnology &amp; Applied Biochemistry&lt;/searchLink&gt;. Dec2025, Vol. 72 Issue 6, p1788-1797. 10p.
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– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The extraction of high‐quality DNA from fungi remains a significant challenge due to the structural complexity and resilience of fungal cell walls. This study presents the first comprehensive comparison of DNA extraction efficiency between blastospores (BS) and mycelial cells (MCs) in dimorphic fungi. We evaluated six DNA extraction procedures and two pre‐treatment methods to determine their effectiveness in extracting DNA of high quality and yield from both forms. DNA quantification was performed using absolute RT‐qPCR targeting the single‐copy γ‐Actin gene, with specificity and efficiency validated through standardized protocols. Our results showed that BS consistently yielded higher DNA quantity and quality across all conditions, with a mean antilog DNA copy concentration of 5.03 &#177; 0.20, compared to 2.51 &#177; 0.20 for MC. DNA quality was superior in BS, as indicated by significantly better 260/280 and 260/230 ratios (31.5% vs. 7.4% for MC, p value = 0.002). Additionally, BS produced higher molecular weight DNA (10,770.5 base pairs vs. 8139.0 base pairs for MC, p value &lt; 0.001) and exhibited greater long‐term stability at both 4&#176;C and −20&#176;C. The results demonstrate that BS serve as a more reliable source of high‐quality DNA compared to MC. This finding enhances current methodologies in molecular biology and supports more accurate analyses, particularly in studies involving dimorphic fungi. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: &lt;i&gt;Copyright of Biotechnology &amp; Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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        Value: 10.1002/bab.2786
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        Text: English
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        PageCount: 10
        StartPage: 1788
    Subjects:
      – SubjectFull: Fungal spores
        Type: general
      – SubjectFull: Fungi
        Type: general
      – SubjectFull: Molecular weights
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      – SubjectFull: Nucleic acid isolation methods
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      – SubjectFull: Agonomycetales
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      – SubjectFull: Statistical reliability
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      – SubjectFull: Polymerase chain reaction
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      – TitleFull: Enhanced DNA Extraction From Dimorphic Fungi: Exploring the Advantages of Blastospores Versus Mycelia.
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              M: 12
              Text: Dec2025
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