Preparation and Characterization of Demineralized Bone Extracellular Matrix/Chitosan Porous Microcarriers.
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| Title: | Preparation and Characterization of Demineralized Bone Extracellular Matrix/Chitosan Porous Microcarriers. |
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| Authors: | Jiang, Jie1 (AUTHOR), Fang, Jianjun1 (AUTHOR) fang_88088@163.com, Liu, Wenjuan2 (AUTHOR), Guo, Meimei1 (AUTHOR), Lu, Zhongjia1 (AUTHOR), Tang, Heng1 (AUTHOR), Lan, Yi3 (AUTHOR), Zhou, Zhihua1,2,4 (AUTHOR) zhou7381@126.com |
| Source: | Journal of Macromolecular Science: Physics. 2026, Vol. 65 Issue 1, p102-114. 13p. |
| Subjects: | Cytocompatibility, Biocompatibility, Scanning electron microscopes, Extracellular matrix, Chitosan, Biomaterials, Freeze-drying, Emulsions |
| Abstract: | Composite porous microcarriers based on demineralized bone extracellular matrix (DBECM) and chitosan (Cs) were prepared using a combined emulsion and freeze-drying method. The effects of digestion by pepsin on the DBECM properties were investigated. The morphologies and porous structure of the DBECM/Cs porous microcarriers were observed by using a scanning electron microscope (SEM). The cytocompatibility of the DBECM/Cs porous microcarriers were studied by using cell counting kit-8 (CCK-8) and calcein acetoxymethyl ester/propidium iodide (CAM/PI). DBECM was digested into small pieces with a loose porous sponge structure and the digested DBECM showed an amorphous state. The resultant DBECM/Cs porous microcarriers showed a spherical shape and an interconnective porous structure. The CCK-8 and CAM/PI analyses indicated that the DBECM/Cs porous microcarriers were non-cytotoxic and showed good biocompatibility, providing a more favorable growth environment for cells. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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| Abstract: | Composite porous microcarriers based on demineralized bone extracellular matrix (DBECM) and chitosan (Cs) were prepared using a combined emulsion and freeze-drying method. The effects of digestion by pepsin on the DBECM properties were investigated. The morphologies and porous structure of the DBECM/Cs porous microcarriers were observed by using a scanning electron microscope (SEM). The cytocompatibility of the DBECM/Cs porous microcarriers were studied by using cell counting kit-8 (CCK-8) and calcein acetoxymethyl ester/propidium iodide (CAM/PI). DBECM was digested into small pieces with a loose porous sponge structure and the digested DBECM showed an amorphous state. The resultant DBECM/Cs porous microcarriers showed a spherical shape and an interconnective porous structure. The CCK-8 and CAM/PI analyses indicated that the DBECM/Cs porous microcarriers were non-cytotoxic and showed good biocompatibility, providing a more favorable growth environment for cells. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00222348 |
| DOI: | 10.1080/00222348.2024.2409526 |
