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Cytocompatibility and Microbiological Effects of Ti6Al4V Particles Generated During Implantoplasty on Human Fibroblasts, Osteoblasts, and Multispecies Oral Biofilm.

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Title: Cytocompatibility and Microbiological Effects of Ti6Al4V Particles Generated During Implantoplasty on Human Fibroblasts, Osteoblasts, and Multispecies Oral Biofilm.
Authors: Vegas-Bustamante, Erika1,2 (AUTHOR), Toledano-Serrabona, Jorge1,2 (AUTHOR), Sánchez-Garcés, María Ángeles1,2,3 (AUTHOR), Figueiredo, Rui1,2,4 (AUTHOR), Demiquels-Punzano, Elena3,5 (AUTHOR), Gil, Javier1,4 (AUTHOR), Delgado, Luis M.2,4,5 (AUTHOR), Sanmartí-García, Gemma1,2,3 (AUTHOR), Camps-Font, Octavi1,2,4 (AUTHOR)
Source: Materials (1996-1944). Dec2025, Vol. 18 Issue 24, p5626. 18p.
Subjects: Cytocompatibility, Fibroblasts, Titanium alloys, Cytotoxins, Osteoblasts, Bacterial growth, Biofilms, Operative surgery
Abstract: Objectives: This study aimed to evaluate the cytotoxic effects of Ti6Al4V particles and implantoplasty (IP)-treated surfaces on human fibroblasts and osteoblasts, and to investigate the influence of these particles on multispecies oral biofilm formation. Methods: Ti6Al4V particles generated during implantoplasty were collected. Human fibroblasts (HFF-1) and osteoblast-like cells (SaOs-2) were used to assess cytotoxicity through indirect lactate dehydrogenase (LDH) assays. Multispecies biofilms composed of Streptococcus oralis, Actinomyces viscosus, Veillonella parvula and Porphyromonas gingivalis were evaluated based on colony-forming units (CFUs) and metabolic activity. Fibroblasts and osteoblasts were co-cultured with biofilm-contaminated particles for 2, 4 and 6 h. Cell morphology and biofilm association were examined by phase-contrast microscopy, while metabolic activity was measured spectrophotometrically. Results: IP-treated surfaces showed no significant cytotoxicity (metabolic activity > 92%, LDH < 20%). Ti6Al4V particles selectively promoted A. viscosus and V. parvula growth (metabolic activity increases of ≈192% and ≈203%; CFU significantly higher versus controls, p < 0.05). Co-culture with biofilm-contaminated particles drastically reduced cell activity (fibroblasts < 25%, osteoblasts < 10%), whereas bacteria-free particles did not. Conclusions: Biofilm-contaminated particles released during implantoplasty markedly impair fibroblast and osteoblast cytocompatibility and selectively alter bacterial growth, whereas IP-treated surfaces per se are biocompatible. Minimizing particle dissemination and bacterial contamination during IP is therefore crucial. [ABSTRACT FROM AUTHOR]
Copyright of Materials (1996-1944) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Cytocompatibility and Microbiological Effects of Ti6Al4V Particles Generated During Implantoplasty on Human Fibroblasts, Osteoblasts, and Multispecies Oral Biofilm.
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  Data: Objectives: This study aimed to evaluate the cytotoxic effects of Ti6Al4V particles and implantoplasty (IP)-treated surfaces on human fibroblasts and osteoblasts, and to investigate the influence of these particles on multispecies oral biofilm formation. Methods: Ti6Al4V particles generated during implantoplasty were collected. Human fibroblasts (HFF-1) and osteoblast-like cells (SaOs-2) were used to assess cytotoxicity through indirect lactate dehydrogenase (LDH) assays. Multispecies biofilms composed of Streptococcus oralis, Actinomyces viscosus, Veillonella parvula and Porphyromonas gingivalis were evaluated based on colony-forming units (CFUs) and metabolic activity. Fibroblasts and osteoblasts were co-cultured with biofilm-contaminated particles for 2, 4 and 6 h. Cell morphology and biofilm association were examined by phase-contrast microscopy, while metabolic activity was measured spectrophotometrically. Results: IP-treated surfaces showed no significant cytotoxicity (metabolic activity &gt; 92%, LDH &lt; 20%). Ti6Al4V particles selectively promoted A. viscosus and V. parvula growth (metabolic activity increases of ≈192% and ≈203%; CFU significantly higher versus controls, p &lt; 0.05). Co-culture with biofilm-contaminated particles drastically reduced cell activity (fibroblasts &lt; 25%, osteoblasts &lt; 10%), whereas bacteria-free particles did not. Conclusions: Biofilm-contaminated particles released during implantoplasty markedly impair fibroblast and osteoblast cytocompatibility and selectively alter bacterial growth, whereas IP-treated surfaces per se are biocompatible. Minimizing particle dissemination and bacterial contamination during IP is therefore crucial. [ABSTRACT FROM AUTHOR]
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  Data: &lt;i&gt;Copyright of Materials (1996-1944) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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        Value: 10.3390/ma18245626
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      – Code: eng
        Text: English
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        PageCount: 18
        StartPage: 5626
    Subjects:
      – SubjectFull: Cytocompatibility
        Type: general
      – SubjectFull: Fibroblasts
        Type: general
      – SubjectFull: Titanium alloys
        Type: general
      – SubjectFull: Cytotoxins
        Type: general
      – SubjectFull: Osteoblasts
        Type: general
      – SubjectFull: Bacterial growth
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      – SubjectFull: Biofilms
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      – SubjectFull: Operative surgery
        Type: general
    Titles:
      – TitleFull: Cytocompatibility and Microbiological Effects of Ti6Al4V Particles Generated During Implantoplasty on Human Fibroblasts, Osteoblasts, and Multispecies Oral Biofilm.
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              Text: Dec2025
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