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A coumarin-thiazole orange conjugate as a near-infrared ratiometric fluorescent probe for G-quadruplex DNA.

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Title: A coumarin-thiazole orange conjugate as a near-infrared ratiometric fluorescent probe for G-quadruplex DNA.
Authors: Fan, Xu-Bo1 (AUTHOR), Zhao, Yi-Ran1 (AUTHOR), Guo, Haoran2 (AUTHOR), Li, Lu-Yu1 (AUTHOR), Li, Ze-Kai1 (AUTHOR), Shu, Jing1 (AUTHOR), Zhou, Si-Hong1 (AUTHOR), Huang, Gui-Chuan1 (AUTHOR), Yang, Fa1,2 (AUTHOR) yangfa@fmmu.edu.cn, Wang, Ming-Qi1 (AUTHOR) wmq3415@163.com
Source: Dyes & Pigments. Apr2026, Vol. 247, pN.PAG-N.PAG. 1p.
Subjects: Fluorescent probes, Quadruplex nucleic acids, Cell imaging, Dual fluorescence, Near infrared spectroscopy, Signal detection
Abstract: Owing to its extremely low fluorescence background and remarkable "turn-on" response, thiazole orange (TO) scaffold has been extensively employed to construct fluorescent probes for G-quadruplex (G4) DNA, which have significant biological importance in numerous cellular processes and therapeutic applications. In contrast to single-wavelength signal changes in "turn-on" probes, ratiometric fluorescent probes based on dual-emission changes can eliminate the errors associated with changes in the local concentration of probes, thereby offer improving detection accuracy and sensitivity. Herein, a coumarin-TO conjugate 4TO-Cur is reported, which serves as a near-infrared (NIR) ratiometric fluorescent probe for G4 DNA structures. Upon binding to G4 DNA, 4TO-Cur exhibits a constant intrinsic emission at 515 nm from its coumarin moiety, while the newly formed fluorescence signal at 680 nm is significantly enhanced. The response mechanism of the 4TO-Cur involves its aggregation upon binding to G4 DNA, which in turn restricts the intramolecular rotation of the probe, leading to intense NIR fluorescence emission. Meanwhile, the coumarin moiety responsible for the green fluorescence remains largely unaffected. Utilizing probe 4TO-Cur , a label-free binary INHIBIT logic gate was successfully constructed, thereby demonstrating its potential for use in molecular-scale logic circuits. Furthermore, the probe was applied in live cells fluorescence imaging, where it could be used to monitor viscosity changes within the cytoplasm. Our work establishes a framework and provides valuable insights for the construction of ratiometric fluorescent probes for G4 DNA. • A NIR ratiometric fluorescent probe named 4TO-Cur was developed for the detection of G4 DNA structures. • 4TO-Cur binds G4 DNAs with high affinity and selectivity, yielding a distinct ratiometric fluorescence response. • The response mechanism has been extensively investigated. • A label-free binary INHIBIT gate was achieved by 4TO-Cur. • The cell compatibility and live cells imaging capability were evaluated. [ABSTRACT FROM AUTHOR]
Copyright of Dyes & Pigments is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 190695723
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: A coumarin-thiazole orange conjugate as a near-infrared ratiometric fluorescent probe for G-quadruplex DNA.
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  Data: <searchLink fieldCode="DE" term="%22Fluorescent+probes%22">Fluorescent probes</searchLink><br /><searchLink fieldCode="DE" term="%22Quadruplex+nucleic+acids%22">Quadruplex nucleic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+imaging%22">Cell imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Dual+fluorescence%22">Dual fluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Near+infrared+spectroscopy%22">Near infrared spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Signal+detection%22">Signal detection</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Owing to its extremely low fluorescence background and remarkable "turn-on" response, thiazole orange (TO) scaffold has been extensively employed to construct fluorescent probes for G-quadruplex (G4) DNA, which have significant biological importance in numerous cellular processes and therapeutic applications. In contrast to single-wavelength signal changes in "turn-on" probes, ratiometric fluorescent probes based on dual-emission changes can eliminate the errors associated with changes in the local concentration of probes, thereby offer improving detection accuracy and sensitivity. Herein, a coumarin-TO conjugate 4TO-Cur is reported, which serves as a near-infrared (NIR) ratiometric fluorescent probe for G4 DNA structures. Upon binding to G4 DNA, 4TO-Cur exhibits a constant intrinsic emission at 515 nm from its coumarin moiety, while the newly formed fluorescence signal at 680 nm is significantly enhanced. The response mechanism of the 4TO-Cur involves its aggregation upon binding to G4 DNA, which in turn restricts the intramolecular rotation of the probe, leading to intense NIR fluorescence emission. Meanwhile, the coumarin moiety responsible for the green fluorescence remains largely unaffected. Utilizing probe 4TO-Cur , a label-free binary INHIBIT logic gate was successfully constructed, thereby demonstrating its potential for use in molecular-scale logic circuits. Furthermore, the probe was applied in live cells fluorescence imaging, where it could be used to monitor viscosity changes within the cytoplasm. Our work establishes a framework and provides valuable insights for the construction of ratiometric fluorescent probes for G4 DNA. • A NIR ratiometric fluorescent probe named 4TO-Cur was developed for the detection of G4 DNA structures. • 4TO-Cur binds G4 DNAs with high affinity and selectivity, yielding a distinct ratiometric fluorescence response. • The response mechanism has been extensively investigated. • A label-free binary INHIBIT gate was achieved by 4TO-Cur. • The cell compatibility and live cells imaging capability were evaluated. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Dyes & Pigments is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.dyepig.2025.113513
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Fluorescent probes
        Type: general
      – SubjectFull: Quadruplex nucleic acids
        Type: general
      – SubjectFull: Cell imaging
        Type: general
      – SubjectFull: Dual fluorescence
        Type: general
      – SubjectFull: Near infrared spectroscopy
        Type: general
      – SubjectFull: Signal detection
        Type: general
    Titles:
      – TitleFull: A coumarin-thiazole orange conjugate as a near-infrared ratiometric fluorescent probe for G-quadruplex DNA.
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            NameFull: Fan, Xu-Bo
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            – D: 01
              M: 04
              Text: Apr2026
              Type: published
              Y: 2026
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