SLIMPHONY: A SLIM-Based Instrument That Orchestrates Complex Ion Mobility–Mass Spectrometry Experiments.
Saved in:
| Title: | SLIMPHONY: A SLIM-Based Instrument That Orchestrates Complex Ion Mobility–Mass Spectrometry Experiments. |
|---|---|
| Authors: | Wageman, AnneClaire1 (AUTHOR), Roush, Addison E.1 (AUTHOR), Feng, Yuan1 (AUTHOR), Bush, Matthew F.1 (AUTHOR) mattbush@uw.edu |
| Source: | Journal of the American Society for Mass Spectrometry. 1/7/2026, Vol. 37 Issue 1, p95-104. 10p. |
| Abstract: | The inherent heterogeneity of biological macromolecules offers a unique challenge for analysis. The combination of ion mobility (IM) and mass spectrometry (MS) is sensitive to the size, shape, and dynamics of, for example, proteins and their complexes. Combining multiple dimensions of ion mobility and mass spectrometry (IM–IM–MS) while leveraging unique gas-phase manipulations between dimensions has great potential for increasing the information content for challenging analytes. Here, we introduce an instrument, SLIMPHONY, which was built using the Structures for Lossless Ion Manipulations (SLIM) architecture. SLIMPHONY is unique in that eight independently controlled traveling-wave regions work in concert to enable complex, multidimensional separations. Single-dimension IM–MS experiments were used to separate a mixture of protein and protein-complex ions and demonstrate that the peak-to-peak resolution increases roughly with the square root of the separation length for a pair of hexakis-(fluoroalkoxy)-phosphazine ions. Ion selection and trapping between dimensions was then used to probe the gas-phase unfolding of a subpopulation of ubiquitin ions. Finally, by varying the guard potential used to confine ions, we demonstrate tunable activation of ubiquitin subpopulations, which we analyzed using IM separations of various lengths. With the ability to select and activate ions in multiple regions, to vary the number of dimensions of IM, and to control the length of IM separation, SLIMPHONY is a flexible platform for characterizing protein ions. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 190770938 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: SLIMPHONY: A SLIM-Based Instrument That Orchestrates Complex Ion Mobility–Mass Spectrometry Experiments. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wageman%2C+AnneClaire%22">Wageman, AnneClaire</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Roush%2C+Addison+E%2E%22">Roush, Addison E.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Feng%2C+Yuan%22">Feng, Yuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bush%2C+Matthew+F%2E%22">Bush, Matthew F.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mattbush@uw.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+the+American+Society+for+Mass+Spectrometry%22">Journal of the American Society for Mass Spectrometry</searchLink>. 1/7/2026, Vol. 37 Issue 1, p95-104. 10p. – Name: Abstract Label: Abstract Group: Ab Data: The inherent heterogeneity of biological macromolecules offers a unique challenge for analysis. The combination of ion mobility (IM) and mass spectrometry (MS) is sensitive to the size, shape, and dynamics of, for example, proteins and their complexes. Combining multiple dimensions of ion mobility and mass spectrometry (IM–IM–MS) while leveraging unique gas-phase manipulations between dimensions has great potential for increasing the information content for challenging analytes. Here, we introduce an instrument, SLIMPHONY, which was built using the Structures for Lossless Ion Manipulations (SLIM) architecture. SLIMPHONY is unique in that eight independently controlled traveling-wave regions work in concert to enable complex, multidimensional separations. Single-dimension IM–MS experiments were used to separate a mixture of protein and protein-complex ions and demonstrate that the peak-to-peak resolution increases roughly with the square root of the separation length for a pair of hexakis-(fluoroalkoxy)-phosphazine ions. Ion selection and trapping between dimensions was then used to probe the gas-phase unfolding of a subpopulation of ubiquitin ions. Finally, by varying the guard potential used to confine ions, we demonstrate tunable activation of ubiquitin subpopulations, which we analyzed using IM separations of various lengths. With the ability to select and activate ions in multiple regions, to vary the number of dimensions of IM, and to control the length of IM separation, SLIMPHONY is a flexible platform for characterizing protein ions. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=190770938 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/jasms.5c00217 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 95 Titles: – TitleFull: SLIMPHONY: A SLIM-Based Instrument That Orchestrates Complex Ion Mobility–Mass Spectrometry Experiments. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wageman, AnneClaire – PersonEntity: Name: NameFull: Roush, Addison E. – PersonEntity: Name: NameFull: Feng, Yuan – PersonEntity: Name: NameFull: Bush, Matthew F. IsPartOfRelationships: – BibEntity: Dates: – D: 07 M: 01 Text: 1/7/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 10440305 Numbering: – Type: volume Value: 37 – Type: issue Value: 1 Titles: – TitleFull: Journal of the American Society for Mass Spectrometry Type: main |
| ResultId | 1 |
