View in EDS

Using pure oxygen aeration to increase recombinant protein production by an Aspergillus oryzae hyphal dispersion strain.

Saved in:
Bibliographic Details
Title: Using pure oxygen aeration to increase recombinant protein production by an Aspergillus oryzae hyphal dispersion strain.
Authors: Ishibashi, Satoshi1 (AUTHOR), Susukida, Shunya1 (AUTHOR), Muto, Kiyoaki1 (AUTHOR), Miyazawa, Ken1,2 (AUTHOR), Zhang, Silai3 (AUTHOR), Yoshimi, Akira2,4,5 (AUTHOR), Tabata, Eiji6 (AUTHOR), Abe, Keietsu1,2,7 (AUTHOR) keietsu.abe.b5@tohoku.ac.jp
Source: Journal of Bioscience & Bioengineering. Feb2026, Vol. 141 Issue 2, p98-107. 10p.
Subjects: Dynamic viscosity, Xylanases, Cell populations, Recombinant proteins, Koji, Fermentation
Abstract: A high growth rate is essential for increasing protein production efficiency in liquid fermentation of filamentous fungi, such as Aspergillus oryzae. However, the increase in culture viscosity due to fungal growth constrains the overall yield. We have demonstrated that culture viscosity is lower in A. oryzae AGΔ-GAGΔ strains, which are deficient in the cell surface polysaccharides α-1,3-glucan (AG) and galactosaminogalactan (GAG), than in the wild-type (WT) strains. Nevertheless, even in aerated fermentation, an increase in AGΔ-GAGΔ viscosity results in oxygen depletion, which limits fungal growth and enzyme production. In this study, we investigated viscosity dynamics and protein production during high-cell-density fermentation of AGΔ-GAGΔ under pure oxygen aeration. Fed-batch cultivation of the WT and AGΔ-GAGΔ strains, expressing recombinant xylanase (XynF1), was used to compare the effects of air and pure oxygen aeration at the same flow rate. At 60 h, AGΔ-GAGΔ under pure oxygen aeration showed higher cell density (1.2× WT under pure oxygen aeration, 2.1× AGΔ-GAGΔ under air aeration) and XynF1 activity (1.8× WT under pure oxygen aeration, 2.3× AGΔ-GAGΔ under air aeration). Under pure oxygen aeration, AGΔ-GAGΔ showed lower viscosity (0.32×) and mixing time (0.50×) than WT. At 60 h, fine mycelial pellets (micropellets; 200–700 μm) were clearly observed in AGΔ-GAGΔ under pure oxygen but not under air aeration. These findings suggest that oxygen enrichment during AGΔ-GAGΔ cultivation mitigated the increase in viscosity, thereby promoting higher cell density and protein production. The formation of micropellets in AGΔ-GAGΔ likely contributed to a reduction in culture viscosity. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 190884057
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Using pure oxygen aeration to increase recombinant protein production by an Aspergillus oryzae hyphal dispersion strain.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Ishibashi%2C+Satoshi%22">Ishibashi, Satoshi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Susukida%2C+Shunya%22">Susukida, Shunya</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Muto%2C+Kiyoaki%22">Muto, Kiyoaki</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Miyazawa%2C+Ken%22">Miyazawa, Ken</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Silai%22">Zhang, Silai</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yoshimi%2C+Akira%22">Yoshimi, Akira</searchLink><relatesTo>2,4,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tabata%2C+Eiji%22">Tabata, Eiji</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Abe%2C+Keietsu%22">Abe, Keietsu</searchLink><relatesTo>1,2,7</relatesTo> (AUTHOR)<i> keietsu.abe.b5@tohoku.ac.jp</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Bioscience+%26+Bioengineering%22">Journal of Bioscience & Bioengineering</searchLink>. Feb2026, Vol. 141 Issue 2, p98-107. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Dynamic+viscosity%22">Dynamic viscosity</searchLink><br /><searchLink fieldCode="DE" term="%22Xylanases%22">Xylanases</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+populations%22">Cell populations</searchLink><br /><searchLink fieldCode="DE" term="%22Recombinant+proteins%22">Recombinant proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Koji%22">Koji</searchLink><br /><searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: A high growth rate is essential for increasing protein production efficiency in liquid fermentation of filamentous fungi, such as Aspergillus oryzae. However, the increase in culture viscosity due to fungal growth constrains the overall yield. We have demonstrated that culture viscosity is lower in A. oryzae AGΔ-GAGΔ strains, which are deficient in the cell surface polysaccharides α-1,3-glucan (AG) and galactosaminogalactan (GAG), than in the wild-type (WT) strains. Nevertheless, even in aerated fermentation, an increase in AGΔ-GAGΔ viscosity results in oxygen depletion, which limits fungal growth and enzyme production. In this study, we investigated viscosity dynamics and protein production during high-cell-density fermentation of AGΔ-GAGΔ under pure oxygen aeration. Fed-batch cultivation of the WT and AGΔ-GAGΔ strains, expressing recombinant xylanase (XynF1), was used to compare the effects of air and pure oxygen aeration at the same flow rate. At 60 h, AGΔ-GAGΔ under pure oxygen aeration showed higher cell density (1.2× WT under pure oxygen aeration, 2.1× AGΔ-GAGΔ under air aeration) and XynF1 activity (1.8× WT under pure oxygen aeration, 2.3× AGΔ-GAGΔ under air aeration). Under pure oxygen aeration, AGΔ-GAGΔ showed lower viscosity (0.32×) and mixing time (0.50×) than WT. At 60 h, fine mycelial pellets (micropellets; 200–700 μm) were clearly observed in AGΔ-GAGΔ under pure oxygen but not under air aeration. These findings suggest that oxygen enrichment during AGΔ-GAGΔ cultivation mitigated the increase in viscosity, thereby promoting higher cell density and protein production. The formation of micropellets in AGΔ-GAGΔ likely contributed to a reduction in culture viscosity. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Bioscience & Bioengineering is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=190884057
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jbiosc.2025.10.011
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 98
    Subjects:
      – SubjectFull: Dynamic viscosity
        Type: general
      – SubjectFull: Xylanases
        Type: general
      – SubjectFull: Cell populations
        Type: general
      – SubjectFull: Recombinant proteins
        Type: general
      – SubjectFull: Koji
        Type: general
      – SubjectFull: Fermentation
        Type: general
    Titles:
      – TitleFull: Using pure oxygen aeration to increase recombinant protein production by an Aspergillus oryzae hyphal dispersion strain.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Ishibashi, Satoshi
      – PersonEntity:
          Name:
            NameFull: Susukida, Shunya
      – PersonEntity:
          Name:
            NameFull: Muto, Kiyoaki
      – PersonEntity:
          Name:
            NameFull: Miyazawa, Ken
      – PersonEntity:
          Name:
            NameFull: Zhang, Silai
      – PersonEntity:
          Name:
            NameFull: Yoshimi, Akira
      – PersonEntity:
          Name:
            NameFull: Tabata, Eiji
      – PersonEntity:
          Name:
            NameFull: Abe, Keietsu
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 02
              Text: Feb2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 13891723
          Numbering:
            – Type: volume
              Value: 141
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: Journal of Bioscience & Bioengineering
              Type: main
ResultId 1