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Aureobasidium pullulans NRRL 62042 as robust platform for efficient lignin valorization and sustainable cis,cis-muconic acid production.

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Title: Aureobasidium pullulans NRRL 62042 as robust platform for efficient lignin valorization and sustainable cis,cis-muconic acid production.
Authors: Driller, Marielle1,2 (AUTHOR) marielle.driller@rwth-aachen.de, Grad, Marius3 (AUTHOR) marius.grad@uni-saarland.de, Dielentheis-Frenken, Marie R.E.2 (AUTHOR) marie.dielentheis-frenken@uni-bielefeld.de, Stein, Karla1,2 (AUTHOR) karla.stein@rwth-aachen.de, Kohlstedt, Michael3 (AUTHOR) michael.kohlstedt@uni-saarland.de, Wittmann, Christoph3 (AUTHOR) christoph.wittmann@uni-saarland.de, Blank, Lars M.1,4 (AUTHOR) lars.blank@rwth-aachen.de, Tiso, Till1,2 (AUTHOR) till.tiso@uni-bielefeld.de
Source: Bioresource Technology. Mar2026, Vol. 443, pN.PAG-N.PAG. 1p.
Subjects: Aureobasidium pullulans, Sustainability, Lignification, Bioreactors, Yeast, Organic acids, Aromatic compounds
Abstract: [Display omitted] • High tolerance of Aureobasidium pullulans towards various lignin-derived monomers. • Growth on complex depolymerized lignins highlights strain robustness. • 23 g L-1 muconic acid with complete catechol conversion in fed-batch fermentation. • Fully sustainable process using lignin hydrolysate and hemicellulose-derived xylose. Lignin is a highly abundant source of renewable aromatics , but efficient microbial conversion remains challenging due to the limited substrate tolerance of many microorganisms. In this study, the polyextremotolerant yeast-like fungus Aureobasidium pullulans NRRL 62042 was identified as a robust host capable of growing on a range of lignin-derived monomers as well as on complex depolymerized lignins. One aromatic monomer generated during lignin depolymerization is catechol, which can be converted into the higher-value product muconic acid (MA) in a single enzymatic step. To channel catechol toward MA, a muconate cycloisomerase I deletion mutant was generated and evaluated in small-scale cultivations. MA production was further enhanced through the implementation of improved feeding strategies. Finally, fed-batch fermentation in a 1.2 L bioreactor showed efficient catechol conversion and production of 23 g L-1 MA. These findings position A. pullulans as a strong platform organism for sustainable lignin valorization and MA production. [ABSTRACT FROM AUTHOR]
Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 190961845
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  Data: Aureobasidium pullulans NRRL 62042 as robust platform for efficient lignin valorization and sustainable cis,cis-muconic acid production.
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  Data: <searchLink fieldCode="AR" term="%22Driller%2C+Marielle%22">Driller, Marielle</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> marielle.driller@rwth-aachen.de</i><br /><searchLink fieldCode="AR" term="%22Grad%2C+Marius%22">Grad, Marius</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> marius.grad@uni-saarland.de</i><br /><searchLink fieldCode="AR" term="%22Dielentheis-Frenken%2C+Marie+R%2EE%2E%22">Dielentheis-Frenken, Marie R.E.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> marie.dielentheis-frenken@uni-bielefeld.de</i><br /><searchLink fieldCode="AR" term="%22Stein%2C+Karla%22">Stein, Karla</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> karla.stein@rwth-aachen.de</i><br /><searchLink fieldCode="AR" term="%22Kohlstedt%2C+Michael%22">Kohlstedt, Michael</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> michael.kohlstedt@uni-saarland.de</i><br /><searchLink fieldCode="AR" term="%22Wittmann%2C+Christoph%22">Wittmann, Christoph</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> christoph.wittmann@uni-saarland.de</i><br /><searchLink fieldCode="AR" term="%22Blank%2C+Lars+M%2E%22">Blank, Lars M.</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<i> lars.blank@rwth-aachen.de</i><br /><searchLink fieldCode="AR" term="%22Tiso%2C+Till%22">Tiso, Till</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> till.tiso@uni-bielefeld.de</i>
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  Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Mar2026, Vol. 443, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Aureobasidium+pullulans%22">Aureobasidium pullulans</searchLink><br /><searchLink fieldCode="DE" term="%22Sustainability%22">Sustainability</searchLink><br /><searchLink fieldCode="DE" term="%22Lignification%22">Lignification</searchLink><br /><searchLink fieldCode="DE" term="%22Bioreactors%22">Bioreactors</searchLink><br /><searchLink fieldCode="DE" term="%22Yeast%22">Yeast</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+acids%22">Organic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Aromatic+compounds%22">Aromatic compounds</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: [Display omitted] • High tolerance of Aureobasidium pullulans towards various lignin-derived monomers. • Growth on complex depolymerized lignins highlights strain robustness. • 23 g L-1 muconic acid with complete catechol conversion in fed-batch fermentation. • Fully sustainable process using lignin hydrolysate and hemicellulose-derived xylose. Lignin is a highly abundant source of renewable aromatics , but efficient microbial conversion remains challenging due to the limited substrate tolerance of many microorganisms. In this study, the polyextremotolerant yeast-like fungus Aureobasidium pullulans NRRL 62042 was identified as a robust host capable of growing on a range of lignin-derived monomers as well as on complex depolymerized lignins. One aromatic monomer generated during lignin depolymerization is catechol, which can be converted into the higher-value product muconic acid (MA) in a single enzymatic step. To channel catechol toward MA, a muconate cycloisomerase I deletion mutant was generated and evaluated in small-scale cultivations. MA production was further enhanced through the implementation of improved feeding strategies. Finally, fed-batch fermentation in a 1.2 L bioreactor showed efficient catechol conversion and production of 23 g L-1 MA. These findings position A. pullulans as a strong platform organism for sustainable lignin valorization and MA production. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.biortech.2025.133851
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Aureobasidium pullulans
        Type: general
      – SubjectFull: Sustainability
        Type: general
      – SubjectFull: Lignification
        Type: general
      – SubjectFull: Bioreactors
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      – SubjectFull: Yeast
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      – SubjectFull: Organic acids
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      – SubjectFull: Aromatic compounds
        Type: general
    Titles:
      – TitleFull: Aureobasidium pullulans NRRL 62042 as robust platform for efficient lignin valorization and sustainable cis,cis-muconic acid production.
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              Text: Mar2026
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              Y: 2026
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