Dynamic 13C‐MRI of Branched‐Chain Amino Acid Metabolism Using Hyperpolarized [1‐13C]2‐Ketoisocaproate: A Multiband IDEAL Spiral Approach in Rodents and Pigs.
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| Title: | Dynamic 13C‐MRI of Branched‐Chain Amino Acid Metabolism Using Hyperpolarized [1‐13C]2‐Ketoisocaproate: A Multiband IDEAL Spiral Approach in Rodents and Pigs. |
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| Authors: | Skre, Ingeborg S.1 (AUTHOR), Christensen, Ditte B.1 (AUTHOR), Karlsson, Magnus1 (AUTHOR), Ardenkjær‐Larsen, Jan H.1 (AUTHOR), Olin, Rie B.1 (AUTHOR), Lerche, Mathilde H.1 (AUTHOR) mhauler@dtu.dk |
| Source: | Magnetic Resonance in Medicine. Apr2026, Vol. 95 Issue 4, p1986-1997. 12p. |
| Subjects: | Branched chain amino acids, Magnetic resonance imaging, Rodents, Scalability, Computer-assisted image analysis (Medicine) |
| Abstract: | Purpose: To develop and validate a dynamic 13C‐MRI acquisition strategy for imaging branched‐chain amino acid (BCAA) metabolism catalyzed by branched‐chain aminotransferase (BCAT) using hyperpolarized [1‐13C]2‐ketoisocaproate (KIC), and to assess its scalability to a large‐animal model. Methods: A multiband spectral‐spatial excitation was combined with IDEAL spiral imaging at 3 T to capture conversion of KIC to [1‐13C]leucine. Hyperpolarized KIC was produced by dissolution dynamic nuclear polarization (dDNP). The method was validated in phantoms mimicking in vivo conditions and applied in mouse brains, where BCAT activity is well characterized. To assess translational feasibility, the protocol was adapted for pig kidneys, a well‐perfused organ with renal physiology and BCAT expression similar to humans. Results: Phantom studies confirmed spectral separation and accurate spatial localization. In mice, the multiband IDEAL spiral sequence generated metabolic maps comparable to chemical‐shift imaging but with higher SNR. Dynamic imaging at 1 s temporal resolution captured reproducible KIC‐to‐leucine conversion across six animals. In pigs, 2 s‐resolution imaging revealed KIC uptake in renal vasculature and cortex followed by cortical leucine accumulation and efflux into the vena cava, reflecting localized metabolism and transport. Conclusion: The presented 13C‐MRI strategy enables efficient spectral separation and high‐temporal‐resolution imaging of KIC metabolism in rodents and pigs, establishing a translatable framework for noninvasive studies of BCAA metabolism. [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 191184111 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Dynamic <superscript>13</superscript>C‐MRI of Branched‐Chain Amino Acid Metabolism Using Hyperpolarized [1‐<superscript>13</superscript>C]2‐Ketoisocaproate: A Multiband IDEAL Spiral Approach in Rodents and Pigs. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Skre%2C+Ingeborg+S%2E%22">Skre, Ingeborg S.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Christensen%2C+Ditte+B%2E%22">Christensen, Ditte B.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Karlsson%2C+Magnus%22">Karlsson, Magnus</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ardenkjær‐Larsen%2C+Jan+H%2E%22">Ardenkjær‐Larsen, Jan H.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Olin%2C+Rie+B%2E%22">Olin, Rie B.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lerche%2C+Mathilde+H%2E%22">Lerche, Mathilde H.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mhauler@dtu.dk</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Apr2026, Vol. 95 Issue 4, p1986-1997. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Branched+chain+amino+acids%22">Branched chain amino acids</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+resonance+imaging%22">Magnetic resonance imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Rodents%22">Rodents</searchLink><br /><searchLink fieldCode="DE" term="%22Scalability%22">Scalability</searchLink><br /><searchLink fieldCode="DE" term="%22Computer-assisted+image+analysis+%28Medicine%29%22">Computer-assisted image analysis (Medicine)</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Purpose: To develop and validate a dynamic 13C‐MRI acquisition strategy for imaging branched‐chain amino acid (BCAA) metabolism catalyzed by branched‐chain aminotransferase (BCAT) using hyperpolarized [1‐13C]2‐ketoisocaproate (KIC), and to assess its scalability to a large‐animal model. Methods: A multiband spectral‐spatial excitation was combined with IDEAL spiral imaging at 3 T to capture conversion of KIC to [1‐13C]leucine. Hyperpolarized KIC was produced by dissolution dynamic nuclear polarization (dDNP). The method was validated in phantoms mimicking in vivo conditions and applied in mouse brains, where BCAT activity is well characterized. To assess translational feasibility, the protocol was adapted for pig kidneys, a well‐perfused organ with renal physiology and BCAT expression similar to humans. Results: Phantom studies confirmed spectral separation and accurate spatial localization. In mice, the multiband IDEAL spiral sequence generated metabolic maps comparable to chemical‐shift imaging but with higher SNR. Dynamic imaging at 1 s temporal resolution captured reproducible KIC‐to‐leucine conversion across six animals. In pigs, 2 s‐resolution imaging revealed KIC uptake in renal vasculature and cortex followed by cortical leucine accumulation and efflux into the vena cava, reflecting localized metabolism and transport. Conclusion: The presented 13C‐MRI strategy enables efficient spectral separation and high‐temporal‐resolution imaging of KIC metabolism in rodents and pigs, establishing a translatable framework for noninvasive studies of BCAA metabolism. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.70167 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1986 Subjects: – SubjectFull: Branched chain amino acids Type: general – SubjectFull: Magnetic resonance imaging Type: general – SubjectFull: Rodents Type: general – SubjectFull: Scalability Type: general – SubjectFull: Computer-assisted image analysis (Medicine) Type: general Titles: – TitleFull: Dynamic 13C‐MRI of Branched‐Chain Amino Acid Metabolism Using Hyperpolarized [1‐13C]2‐Ketoisocaproate: A Multiband IDEAL Spiral Approach in Rodents and Pigs. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Skre, Ingeborg S. – PersonEntity: Name: NameFull: Christensen, Ditte B. – PersonEntity: Name: NameFull: Karlsson, Magnus – PersonEntity: Name: NameFull: Ardenkjær‐Larsen, Jan H. – PersonEntity: Name: NameFull: Olin, Rie B. – PersonEntity: Name: NameFull: Lerche, Mathilde H. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 95 – Type: issue Value: 4 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
| ResultId | 1 |
