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Portable glyphosate detection using metal-catechol dye colorimetric assays combined with a new smartphone detection platform.

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Title: Portable glyphosate detection using metal-catechol dye colorimetric assays combined with a new smartphone detection platform.
Authors: Stopper, Pascal1 (AUTHOR), Konrad, Marcel1 (AUTHOR), Grottke, Nina1 (AUTHOR), Huhn, Carolin1 (AUTHOR) carolin.huhn@uni-tuebingen.de
Source: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. May2026, Vol. 353, pN.PAG-N.PAG. 1p.
Subjects: Glyphosate, Calibration, Biological assay, Spectrophotometry, Ultraviolet-visible spectroscopy, Herbicides
Abstract: Simple and cost-effective glyphosate assays were developed based on competitive demetallation using the dyes pyrocatechol violet (PV) and zincon (ZCN). The dyes served as colorimetric probes in the form of metal-catechol dye complexes: [Cu 2 IIPV] at pH 6.5 and [ZnIIZCN] at pH 7.4 or pH 9.0. UV–Vis spectroscopy measurements were used to monitor complex formation and demetallation (taken to determine optimal reaction media), and to assess and optimize assay stability (with cooling shown to retard probe decomposition by up to a factor of 17). Subsequently, assay selectivity was evaluated based on complex stability constants to assess interference from complexing agents that may be present in real samples. This approach can be used to judge the applicability of the assays to real samples. Finally, calibration curves from large-scale UV–Vis measurements yielded low detection limits of 0.80–1.91 μM (0.14–0.32 mg/L). Most importantly, a novel smartphone-based colorimetric detection system was developed. It is the first of its kind to combine all features necessary for a convenient and user-friendly smartphone detection platform. Key novelties include the field-ready controlled lighting enclosure (3D-printed cuvette holder within a photo box), robust image calibration, automated color extraction based on markers, simultaneous evaluation of a large number of images with an automated output of annotated images, and a user-friendly color analysis software. The Blue proportion = B/(R + G + B) parameter was identified as optimal, with detection limits of 0.87–1.68 μM (0.15–0.28 mg/L) using [ZnIIZCN] at pH 7.4, being comparable to results wit a commercial UV–Vis spectrometer. [Display omitted] • Optimal reaction media for low-cost catechol dye-based assays. • Retardation of probe decomposition by additives and cooling. • Novel evaluation approach of selectivity based on complex stability constants. • All-in-one portable assay with automated smartphone readout and image calibration. • Validation of the colorimetric assays with commercial herbicides. [ABSTRACT FROM AUTHOR]
Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 192083749
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: Portable glyphosate detection using metal-catechol dye colorimetric assays combined with a new smartphone detection platform.
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  Data: <searchLink fieldCode="AR" term="%22Stopper%2C+Pascal%22">Stopper, Pascal</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Konrad%2C+Marcel%22">Konrad, Marcel</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Grottke%2C+Nina%22">Grottke, Nina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huhn%2C+Carolin%22">Huhn, Carolin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> carolin.huhn@uni-tuebingen.de</i>
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  Data: <searchLink fieldCode="JN" term="%22Spectrochimica+Acta+Part+A%3A+Molecular+%26+Biomolecular+Spectroscopy%22">Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy</searchLink>. May2026, Vol. 353, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Glyphosate%22">Glyphosate</searchLink><br /><searchLink fieldCode="DE" term="%22Calibration%22">Calibration</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+assay%22">Biological assay</searchLink><br /><searchLink fieldCode="DE" term="%22Spectrophotometry%22">Spectrophotometry</searchLink><br /><searchLink fieldCode="DE" term="%22Ultraviolet-visible+spectroscopy%22">Ultraviolet-visible spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Herbicides%22">Herbicides</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Simple and cost-effective glyphosate assays were developed based on competitive demetallation using the dyes pyrocatechol violet (PV) and zincon (ZCN). The dyes served as colorimetric probes in the form of metal-catechol dye complexes: [Cu 2 IIPV] at pH 6.5 and [ZnIIZCN] at pH 7.4 or pH 9.0. UV–Vis spectroscopy measurements were used to monitor complex formation and demetallation (taken to determine optimal reaction media), and to assess and optimize assay stability (with cooling shown to retard probe decomposition by up to a factor of 17). Subsequently, assay selectivity was evaluated based on complex stability constants to assess interference from complexing agents that may be present in real samples. This approach can be used to judge the applicability of the assays to real samples. Finally, calibration curves from large-scale UV–Vis measurements yielded low detection limits of 0.80–1.91 μM (0.14–0.32 mg/L). Most importantly, a novel smartphone-based colorimetric detection system was developed. It is the first of its kind to combine all features necessary for a convenient and user-friendly smartphone detection platform. Key novelties include the field-ready controlled lighting enclosure (3D-printed cuvette holder within a photo box), robust image calibration, automated color extraction based on markers, simultaneous evaluation of a large number of images with an automated output of annotated images, and a user-friendly color analysis software. The Blue proportion = B/(R + G + B) parameter was identified as optimal, with detection limits of 0.87–1.68 μM (0.15–0.28 mg/L) using [ZnIIZCN] at pH 7.4, being comparable to results wit a commercial UV–Vis spectrometer. [Display omitted] • Optimal reaction media for low-cost catechol dye-based assays. • Retardation of probe decomposition by additives and cooling. • Novel evaluation approach of selectivity based on complex stability constants. • All-in-one portable assay with automated smartphone readout and image calibration. • Validation of the colorimetric assays with commercial herbicides. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.saa.2026.127586
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Glyphosate
        Type: general
      – SubjectFull: Calibration
        Type: general
      – SubjectFull: Biological assay
        Type: general
      – SubjectFull: Spectrophotometry
        Type: general
      – SubjectFull: Ultraviolet-visible spectroscopy
        Type: general
      – SubjectFull: Herbicides
        Type: general
    Titles:
      – TitleFull: Portable glyphosate detection using metal-catechol dye colorimetric assays combined with a new smartphone detection platform.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Stopper, Pascal
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            NameFull: Konrad, Marcel
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            NameFull: Grottke, Nina
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            NameFull: Huhn, Carolin
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          Dates:
            – D: 15
              M: 05
              Text: May2026
              Type: published
              Y: 2026
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            – Type: volume
              Value: 353
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            – TitleFull: Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
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