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Similar developmental outcomes of salamander larvae produced with fresh and cryopreserved sperm: implications for amphibian conservation.

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Title: Similar developmental outcomes of salamander larvae produced with fresh and cryopreserved sperm: implications for amphibian conservation.
Authors: Lampert, Shaina1 (AUTHOR) shaina.lampert@nczoo.org, Burger, Isabella2 (AUTHOR) iburger@unc.edu, Chen, Devin2 (AUTHOR) dchen@torontozoo.ca, Kouba, Andy2 (AUTHOR) a.kouba@msstate.edu, Kouba, Carrie1 (AUTHOR) ckv7@msstate.edu
Source: Cryobiology. Mar2026, Vol. 122, pN.PAG-N.PAG. 1p.
Subjects: Amphibian conservation, Amphibian larvae, Salamanders, Metamorphosis, Fertilization (Biology), Conservation genetics, Frozen semen, Embryology
Abstract: Sperm cryopreservation is an important tool for the genetic management of threatened amphibian species within conservation breeding programs. Yet, little is known about the growth and development of offspring generated using frozen-thawed sperm compared to those produced using fresh sperm. The objective of this study was to compare the fertilization rate, embryo development, morphology, and survival of tiger salamander (Ambystoma tigrinum) offspring generated using either fresh or frozen-thawed sperm. Captive male and female A. tigrinum were administered exogenous hormones to stimulate gamete production and release. Eggs were collected from females and fertilized with either freshly collected sperm from captive males or frozen-thawed sperm maintained in the U.S. National Amphibian Genome Bank. Following in-vitro fertilization, egg fertilization rates and early embryo development were monitored through hatching. Larvae from each sperm type were then maintained under standardized rearing conditions and routinely monitored for total length, head width, survival, and development through metamorphosis. Results showed no difference in larval growth (p > 0.42), survival rate (p = 0.72), or size at metamorphosis (p > 0.26) between offspring generated from fresh or frozen-thawed sperm. These findings support sperm cryopreservation as an effective conservation tool for producing viable individuals in amphibian conservation programs. [Display omitted] • Cryopreserved and freshly collected sperm yielded similar fertilization rates. • Larval growth and development were not different between cryopreserved and fresh sperm. • Post-metamorphic morphology was similar in cryo- and fresh-produced offspring. • Cryo-produced offspring are viable and can be used to aid breeding and reintroduction. [ABSTRACT FROM AUTHOR]
Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 192152412
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Items – Name: Title
  Label: Title
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  Data: Similar developmental outcomes of salamander larvae produced with fresh and cryopreserved sperm: implications for amphibian conservation.
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  Data: <searchLink fieldCode="AR" term="%22Lampert%2C+Shaina%22">Lampert, Shaina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> shaina.lampert@nczoo.org</i><br /><searchLink fieldCode="AR" term="%22Burger%2C+Isabella%22">Burger, Isabella</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> iburger@unc.edu</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Devin%22">Chen, Devin</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> dchen@torontozoo.ca</i><br /><searchLink fieldCode="AR" term="%22Kouba%2C+Andy%22">Kouba, Andy</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> a.kouba@msstate.edu</i><br /><searchLink fieldCode="AR" term="%22Kouba%2C+Carrie%22">Kouba, Carrie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ckv7@msstate.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Cryobiology%22">Cryobiology</searchLink>. Mar2026, Vol. 122, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Amphibian+conservation%22">Amphibian conservation</searchLink><br /><searchLink fieldCode="DE" term="%22Amphibian+larvae%22">Amphibian larvae</searchLink><br /><searchLink fieldCode="DE" term="%22Salamanders%22">Salamanders</searchLink><br /><searchLink fieldCode="DE" term="%22Metamorphosis%22">Metamorphosis</searchLink><br /><searchLink fieldCode="DE" term="%22Fertilization+%28Biology%29%22">Fertilization (Biology)</searchLink><br /><searchLink fieldCode="DE" term="%22Conservation+genetics%22">Conservation genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Frozen+semen%22">Frozen semen</searchLink><br /><searchLink fieldCode="DE" term="%22Embryology%22">Embryology</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Sperm cryopreservation is an important tool for the genetic management of threatened amphibian species within conservation breeding programs. Yet, little is known about the growth and development of offspring generated using frozen-thawed sperm compared to those produced using fresh sperm. The objective of this study was to compare the fertilization rate, embryo development, morphology, and survival of tiger salamander (Ambystoma tigrinum) offspring generated using either fresh or frozen-thawed sperm. Captive male and female A. tigrinum were administered exogenous hormones to stimulate gamete production and release. Eggs were collected from females and fertilized with either freshly collected sperm from captive males or frozen-thawed sperm maintained in the U.S. National Amphibian Genome Bank. Following in-vitro fertilization, egg fertilization rates and early embryo development were monitored through hatching. Larvae from each sperm type were then maintained under standardized rearing conditions and routinely monitored for total length, head width, survival, and development through metamorphosis. Results showed no difference in larval growth (p > 0.42), survival rate (p = 0.72), or size at metamorphosis (p > 0.26) between offspring generated from fresh or frozen-thawed sperm. These findings support sperm cryopreservation as an effective conservation tool for producing viable individuals in amphibian conservation programs. [Display omitted] • Cryopreserved and freshly collected sperm yielded similar fertilization rates. • Larval growth and development were not different between cryopreserved and fresh sperm. • Post-metamorphic morphology was similar in cryo- and fresh-produced offspring. • Cryo-produced offspring are viable and can be used to aid breeding and reintroduction. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.cryobiol.2026.105593
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Amphibian conservation
        Type: general
      – SubjectFull: Amphibian larvae
        Type: general
      – SubjectFull: Salamanders
        Type: general
      – SubjectFull: Metamorphosis
        Type: general
      – SubjectFull: Fertilization (Biology)
        Type: general
      – SubjectFull: Conservation genetics
        Type: general
      – SubjectFull: Frozen semen
        Type: general
      – SubjectFull: Embryology
        Type: general
    Titles:
      – TitleFull: Similar developmental outcomes of salamander larvae produced with fresh and cryopreserved sperm: implications for amphibian conservation.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Lampert, Shaina
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            NameFull: Burger, Isabella
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            NameFull: Chen, Devin
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            NameFull: Kouba, Andy
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            NameFull: Kouba, Carrie
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          Dates:
            – D: 01
              M: 03
              Text: Mar2026
              Type: published
              Y: 2026
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              Value: 00112240
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              Value: 122
          Titles:
            – TitleFull: Cryobiology
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