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Natural deep eutectic solvents (NADESs) as cryoprotectant for Lactiplantibacillus plantarum YR07: antifreeze mechanisms and applications.

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Title: Natural deep eutectic solvents (NADESs) as cryoprotectant for Lactiplantibacillus plantarum YR07: antifreeze mechanisms and applications.
Authors: Zheng, Sha sha1,2 (AUTHOR), Tan, Li jun1,2 (AUTHOR), Pan, Qiong1,2 (AUTHOR), Zhang, Yu fei1,2 (AUTHOR), Zhang, Wen di1,2 (AUTHOR), Zou, Li fang1,2 (AUTHOR), Cui, Wei1,2 (AUTHOR), Zhou, Yu1,2 (AUTHOR), Hu, Ying ying1,2 (AUTHOR), Xu, Bao cai1,2 (AUTHOR) baocaixu@163.com
Source: Food Research International. May2026:Part 1, Vol. 231, pN.PAG-N.PAG. 1p.
Subjects: Cryoprotective agents, Lactobacillus plantarum, Solvents, Freeze-drying, Quorum sensing, Lipid metabolism, Skim milk
Abstract: Low-temperature and dehydration during freeze-drying severely impaired the viability of Lactiplantibacillus plantarum. This study evaluated the protective effects and underlying mechanisms of a novel cryoprotectant combining natural deep eutectic solvents (NADESs) with skim milk. Systematic comparisons were made among groups treated with NADESs alone (N), skim milk alone (M), or their composite (NM). Analyses included viability assays, gene expression, membrane fatty acid composition, and cell wall integrity. The NM group showed the highest survival rate (86.26 ± 2.02%). The composite's efficacy was attributed to a multi-level synergistic mechanism: physically, a dense NADES-protein matrix inhibited ice-crystal damage, molecularly, NADESs likely stabilized milk proteins and membrane integrity, metabolically, skim milk nutrients drove key stress-response pathways—methionine-quorum sensing, fatty acid metabolism, and active cell wall synthesis. This work first reveals a "methionine metabolism–quorum sensing–fatty acid metabolism–cell wall synthesis" synergistic network, providing a theoretical basis for designing advanced microbial cryoprotectants. [Display omitted] • NADESs-skim milk composite cryoprotectant was developed for LAB. • NADESs activated the QS system to enhance cold stress resistance in probiotics. • A "Met-Acc-Fab-LuxS" synergistic stress-resistant network was revealed. [ABSTRACT FROM AUTHOR]
Copyright of Food Research International is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 192174957
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  Data: Natural deep eutectic solvents (NADESs) as cryoprotectant for Lactiplantibacillus plantarum YR07: antifreeze mechanisms and applications.
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  Data: <searchLink fieldCode="AR" term="%22Zheng%2C+Sha+sha%22">Zheng, Sha sha</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tan%2C+Li+jun%22">Tan, Li jun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pan%2C+Qiong%22">Pan, Qiong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yu+fei%22">Zhang, Yu fei</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Wen+di%22">Zhang, Wen di</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zou%2C+Li+fang%22">Zou, Li fang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cui%2C+Wei%22">Cui, Wei</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Yu%22">Zhou, Yu</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hu%2C+Ying+ying%22">Hu, Ying ying</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xu%2C+Bao+cai%22">Xu, Bao cai</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> baocaixu@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Food+Research+International%22">Food Research International</searchLink>. May2026:Part 1, Vol. 231, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Cryoprotective+agents%22">Cryoprotective agents</searchLink><br /><searchLink fieldCode="DE" term="%22Lactobacillus+plantarum%22">Lactobacillus plantarum</searchLink><br /><searchLink fieldCode="DE" term="%22Solvents%22">Solvents</searchLink><br /><searchLink fieldCode="DE" term="%22Freeze-drying%22">Freeze-drying</searchLink><br /><searchLink fieldCode="DE" term="%22Quorum+sensing%22">Quorum sensing</searchLink><br /><searchLink fieldCode="DE" term="%22Lipid+metabolism%22">Lipid metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Skim+milk%22">Skim milk</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Low-temperature and dehydration during freeze-drying severely impaired the viability of Lactiplantibacillus plantarum. This study evaluated the protective effects and underlying mechanisms of a novel cryoprotectant combining natural deep eutectic solvents (NADESs) with skim milk. Systematic comparisons were made among groups treated with NADESs alone (N), skim milk alone (M), or their composite (NM). Analyses included viability assays, gene expression, membrane fatty acid composition, and cell wall integrity. The NM group showed the highest survival rate (86.26 ± 2.02%). The composite's efficacy was attributed to a multi-level synergistic mechanism: physically, a dense NADES-protein matrix inhibited ice-crystal damage, molecularly, NADESs likely stabilized milk proteins and membrane integrity, metabolically, skim milk nutrients drove key stress-response pathways—methionine-quorum sensing, fatty acid metabolism, and active cell wall synthesis. This work first reveals a "methionine metabolism–quorum sensing–fatty acid metabolism–cell wall synthesis" synergistic network, providing a theoretical basis for designing advanced microbial cryoprotectants. [Display omitted] • NADESs-skim milk composite cryoprotectant was developed for LAB. • NADESs activated the QS system to enhance cold stress resistance in probiotics. • A "Met-Acc-Fab-LuxS" synergistic stress-resistant network was revealed. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Food Research International is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.foodres.2026.118647
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Cryoprotective agents
        Type: general
      – SubjectFull: Lactobacillus plantarum
        Type: general
      – SubjectFull: Solvents
        Type: general
      – SubjectFull: Freeze-drying
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      – SubjectFull: Quorum sensing
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      – SubjectFull: Lipid metabolism
        Type: general
      – SubjectFull: Skim milk
        Type: general
    Titles:
      – TitleFull: Natural deep eutectic solvents (NADESs) as cryoprotectant for Lactiplantibacillus plantarum YR07: antifreeze mechanisms and applications.
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              M: 05
              Text: May2026:Part 1
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              Y: 2026
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