Optimizing cryopreservation protocols of Saccharomyces eubayanus using heat transfer modeling.
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| Title: | Optimizing cryopreservation protocols of Saccharomyces eubayanus using heat transfer modeling. |
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| Authors: | Caruso, María Agustina1 (AUTHOR), Libkind, Diego1 (AUTHOR), Zaritzky, Noemí2 (AUTHOR), Santos, María Victoria1 (AUTHOR) mvsantos@comahue-conicet.gob.ar |
| Source: | Applied Microbiology & Biotechnology. 3/13/2026, Vol. 110 Issue 1, p1-16. 16p. |
| Subjects: | Cryopreservation of cells, Computer simulation of heat transfer, Cell survival, Finite element method, Cold (Temperature), Saccharomyces, Cryopreservation of organs, tissues, etc. |
| Abstract: | Efficient cryopreservation is essential for maintaining the viability of Saccharomyces eubayanus, a cryotolerant wild yeast of industrial importance as the cold-adapted parent of Saccharomyces pastorianus. This study integrated post-thaw viability and vitality assessments of cryopreserved S.eubayanus CRUB 1568ᵀ with experimental measurements of transient heat transfer and numerical simulation of the freezing stage. Two protocols were evaluated: (A) direct freezing of cryovials in cryoboxes at − 80 °C, governed by convection, and (B) freezing inside a CoolCell® device (Corning Inc., Corning, NY, USA), where heat transfer occurs by conduction through the insulated plastic material. A mathematical model was developed to numerically solve the transient heat transfer equation with phase change using the finite element method. Experimental temperature–time data validated the simulations, allowing estimation of overall heat transfer coefficients (UA = 18.04 W m−2 K−1; UB = 4.76 W m−2 K−1) and characteristic freezing times (t회 = 10.9 min; 27.6 min, respectively). Calculated Biot numbers confirmed uniform temperature distribution within cryovials. PROTOCOL A achieved optimal cooling rates (5–7 °C min−1) and yielded higher post-thaw viability (71.7 ± 3.5%) compared with PROTOCOL B (51.2 ± 3.6%) after 1 year at − 80 °C. The integration of modeling and experimental data demonstrates that the overall heat transfer coefficient is a key engineering parameter influencing cryopreservation performance. Direct freezing of cryovials in cryoboxes represents a simpler, faster, and lower-cost approach that ensures uniform cooling and higher cell survival, providing a valuable basis for standardizing yeast cryogenic storage in industrial and biotechnological applications. Key points: Finite element modeling assessed heat transfer during yeast cryopreservation. Direct freezing in cryoboxes achieved higher viability than CoolCell®. Overall heat transfer coefficient (U) is key for cryogenic performance analysis. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 192343628 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Optimizing cryopreservation protocols of Saccharomyces eubayanus using heat transfer modeling. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Caruso%2C+María+Agustina%22">Caruso, María Agustina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Libkind%2C+Diego%22">Libkind, Diego</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zaritzky%2C+Noemí%22">Zaritzky, Noemí</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Santos%2C+María+Victoria%22">Santos, María Victoria</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mvsantos@comahue-conicet.gob.ar</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. 3/13/2026, Vol. 110 Issue 1, p1-16. 16p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cryopreservation+of+cells%22">Cryopreservation of cells</searchLink><br /><searchLink fieldCode="DE" term="%22Computer+simulation+of+heat+transfer%22">Computer simulation of heat transfer</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+survival%22">Cell survival</searchLink><br /><searchLink fieldCode="DE" term="%22Finite+element+method%22">Finite element method</searchLink><br /><searchLink fieldCode="DE" term="%22Cold+%28Temperature%29%22">Cold (Temperature)</searchLink><br /><searchLink fieldCode="DE" term="%22Saccharomyces%22">Saccharomyces</searchLink><br /><searchLink fieldCode="DE" term="%22Cryopreservation+of+organs%2C+tissues%2C+etc%2E%22">Cryopreservation of organs, tissues, etc.</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Efficient cryopreservation is essential for maintaining the viability of Saccharomyces eubayanus, a cryotolerant wild yeast of industrial importance as the cold-adapted parent of Saccharomyces pastorianus. This study integrated post-thaw viability and vitality assessments of cryopreserved S.eubayanus CRUB 1568ᵀ with experimental measurements of transient heat transfer and numerical simulation of the freezing stage. Two protocols were evaluated: (A) direct freezing of cryovials in cryoboxes at − 80 °C, governed by convection, and (B) freezing inside a CoolCell® device (Corning Inc., Corning, NY, USA), where heat transfer occurs by conduction through the insulated plastic material. A mathematical model was developed to numerically solve the transient heat transfer equation with phase change using the finite element method. Experimental temperature–time data validated the simulations, allowing estimation of overall heat transfer coefficients (UA = 18.04 W m−2 K−1; UB = 4.76 W m−2 K−1) and characteristic freezing times (t회 = 10.9 min; 27.6 min, respectively). Calculated Biot numbers confirmed uniform temperature distribution within cryovials. PROTOCOL A achieved optimal cooling rates (5–7 °C min−1) and yielded higher post-thaw viability (71.7 ± 3.5%) compared with PROTOCOL B (51.2 ± 3.6%) after 1 year at − 80 °C. The integration of modeling and experimental data demonstrates that the overall heat transfer coefficient is a key engineering parameter influencing cryopreservation performance. Direct freezing of cryovials in cryoboxes represents a simpler, faster, and lower-cost approach that ensures uniform cooling and higher cell survival, providing a valuable basis for standardizing yeast cryogenic storage in industrial and biotechnological applications. Key points: Finite element modeling assessed heat transfer during yeast cryopreservation. Direct freezing in cryoboxes achieved higher viability than CoolCell®. Overall heat transfer coefficient (U) is key for cryogenic performance analysis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-026-13779-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 16 StartPage: 1 Subjects: – SubjectFull: Cryopreservation of cells Type: general – SubjectFull: Computer simulation of heat transfer Type: general – SubjectFull: Cell survival Type: general – SubjectFull: Finite element method Type: general – SubjectFull: Cold (Temperature) Type: general – SubjectFull: Saccharomyces Type: general – SubjectFull: Cryopreservation of organs, tissues, etc. Type: general Titles: – TitleFull: Optimizing cryopreservation protocols of Saccharomyces eubayanus using heat transfer modeling. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Caruso, María Agustina – PersonEntity: Name: NameFull: Libkind, Diego – PersonEntity: Name: NameFull: Zaritzky, Noemí – PersonEntity: Name: NameFull: Santos, María Victoria IsPartOfRelationships: – BibEntity: Dates: – D: 13 M: 03 Text: 3/13/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 110 – Type: issue Value: 1 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
