View in EDS

Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645.

Saved in:
Bibliographic Details
Title: Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645.
Authors: Pu, Yang1 (AUTHOR), Liu, Chuanxu1 (AUTHOR), Lan, Xiangyu1 (AUTHOR), Xiao, Chao2 (AUTHOR), Xie, Mingyuan3 (AUTHOR) xiemy5@mail.sysu.edu.cn, Li, Wenjun4 (AUTHOR) wjli@yic.ac.cn, Chen, Min5 (AUTHOR) chenmclm@163.com
Source: Journal of Fluorescence. Mar2026, Vol. 36 Issue 3, p2535-2546. 12p.
Subjects: Energy transfer, Time-resolved spectroscopy, Protein fractionation, Photosynthesis, Fluorescence spectroscopy, Phycocyanin, Chromatographic analysis
Abstract: As a remarkable model protein for studying excitation energy transfer (EET), Cryptophyte phycocyanin 645 (Cr-PC645) is not only important in the frontier research field on photosynthesis but also, like other kinds of phycocyanin, valuable in food and cosmetic industries as a kind of natural blue pigment protein. Here, we report a convenient preparation method for the purification of Cr-PC645 from cryptophytic algae, Chroomonas placoidea, mainly using hydrophobic interaction chromatography, and a study of EET characterization based on it. The final recovery of Cr-PC645 is 58.49% and the highest purity of final samples reaches 7.27 (A645/A280) in the obtained products. In addition to high purity, the properties of unchanged intact optical activity of purified Cr-PC645 are proved by comprehensively analyzing absorption, fluorescence, circular dichroism, and ultrafast time-resolved spectra. Furthermore, three time components of 6, 59, and 1021 ps are confirmed by 77 K time-resolved fluorescence spectroscopy experiments, which can be assigned to the time of energy transfer process between specific phycocyanobilins (PCBs), suggesting that the excitation energy is transferred from PCB-158 to PCB-82 in the same β subunit and localized the PCB-82 chromophores between different β subunits, then transferred to the downstream acceptors. In view of all these results, this optimized method is efficient and will be conducive to multi-field applications in the future. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Fluorescence is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 192346992
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Pu%2C+Yang%22">Pu, Yang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Chuanxu%22">Liu, Chuanxu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lan%2C+Xiangyu%22">Lan, Xiangyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xiao%2C+Chao%22">Xiao, Chao</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xie%2C+Mingyuan%22">Xie, Mingyuan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> xiemy5@mail.sysu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Wenjun%22">Li, Wenjun</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> wjli@yic.ac.cn</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Min%22">Chen, Min</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> chenmclm@163.com</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Fluorescence%22">Journal of Fluorescence</searchLink>. Mar2026, Vol. 36 Issue 3, p2535-2546. 12p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Energy+transfer%22">Energy transfer</searchLink><br /><searchLink fieldCode="DE" term="%22Time-resolved+spectroscopy%22">Time-resolved spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+fractionation%22">Protein fractionation</searchLink><br /><searchLink fieldCode="DE" term="%22Photosynthesis%22">Photosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+spectroscopy%22">Fluorescence spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Phycocyanin%22">Phycocyanin</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: As a remarkable model protein for studying excitation energy transfer (EET), Cryptophyte phycocyanin 645 (Cr-PC645) is not only important in the frontier research field on photosynthesis but also, like other kinds of phycocyanin, valuable in food and cosmetic industries as a kind of natural blue pigment protein. Here, we report a convenient preparation method for the purification of Cr-PC645 from cryptophytic algae, Chroomonas placoidea, mainly using hydrophobic interaction chromatography, and a study of EET characterization based on it. The final recovery of Cr-PC645 is 58.49% and the highest purity of final samples reaches 7.27 (A645/A280) in the obtained products. In addition to high purity, the properties of unchanged intact optical activity of purified Cr-PC645 are proved by comprehensively analyzing absorption, fluorescence, circular dichroism, and ultrafast time-resolved spectra. Furthermore, three time components of 6, 59, and 1021 ps are confirmed by 77 K time-resolved fluorescence spectroscopy experiments, which can be assigned to the time of energy transfer process between specific phycocyanobilins (PCBs), suggesting that the excitation energy is transferred from PCB-158 to PCB-82 in the same β subunit and localized the PCB-82 chromophores between different β subunits, then transferred to the downstream acceptors. In view of all these results, this optimized method is efficient and will be conducive to multi-field applications in the future. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Fluorescence is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=192346992
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s10895-025-04663-7
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 2535
    Subjects:
      – SubjectFull: Energy transfer
        Type: general
      – SubjectFull: Time-resolved spectroscopy
        Type: general
      – SubjectFull: Protein fractionation
        Type: general
      – SubjectFull: Photosynthesis
        Type: general
      – SubjectFull: Fluorescence spectroscopy
        Type: general
      – SubjectFull: Phycocyanin
        Type: general
      – SubjectFull: Chromatographic analysis
        Type: general
    Titles:
      – TitleFull: Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Pu, Yang
      – PersonEntity:
          Name:
            NameFull: Liu, Chuanxu
      – PersonEntity:
          Name:
            NameFull: Lan, Xiangyu
      – PersonEntity:
          Name:
            NameFull: Xiao, Chao
      – PersonEntity:
          Name:
            NameFull: Xie, Mingyuan
      – PersonEntity:
          Name:
            NameFull: Li, Wenjun
      – PersonEntity:
          Name:
            NameFull: Chen, Min
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 03
              Text: Mar2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 10530509
          Numbering:
            – Type: volume
              Value: 36
            – Type: issue
              Value: 3
          Titles:
            – TitleFull: Journal of Fluorescence
              Type: main
ResultId 1