Multi-taxon environmental DNA analysis reveals distributions and pathways of Pelophylax nigromaculatus invasion in Iwamizawa, Hokkaido, Japan.
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| Title: | Multi-taxon environmental DNA analysis reveals distributions and pathways of Pelophylax nigromaculatus invasion in Iwamizawa, Hokkaido, Japan. |
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| Authors: | Nakashima, Naohisa1 (AUTHOR) nnakashima@obihiro.ac.jp, Fukuda, Shinji2,3 (AUTHOR) shinji-f@cc.tuat.ac.jp, Matsubara, Hinata4 (AUTHOR), Ogawa, Kosei4 (AUTHOR), Koshiyama, Naoko5 (AUTHOR) |
| Source: | Hydrobiologia. Apr2026, Vol. 853 Issue 7, p2075-2091. 17p. |
| Subjects: | Dispersal (Ecology), Ditches, Introduced species, Amphibian conservation, Nucleic acids, Habitat conservation, Frogs |
| Geographic Terms: | Hokkaido (Japan), Japan |
| Abstract: | This study explores the recent expansion of the translocated invasive Black-spotted pond frog Pelophylax nigromaculatus in Iwamizawa, Hokkaido, Japan, focusing on dispersal routes and habitat uses at landscape and field scales. Environmental DNA (eDNA) surveys across rivers and agricultural canals confirmed the occurrence of P. nigromaculatus in Iwamizawa for the first time, and revealed that P. nigromaculatus primarily dispersed via agricultural canals connected to paddy fields rather than larger natural rivers. Smaller canals facilitated species movement, whereas rivers posed potential barriers. Detailed eDNA analysis and visual observations in two adjacent paddy lots—one with an open-ditch system and the other without—demonstrated that frogs likely entered from the irrigation canals. The open-ditch lot supported a population due to continuous inundation even during midseason drainage, whereas reduced water levels in the conventional paddy lot restricted long-term microhabitat use. In contrast to the invasive species, the native Japanese tree frog Dryophytes japonicus was not detected by eDNA in the open-ditch lot, reflecting a habitat segregation of the species. These findings support the important role of agricultural canal networks and connectivity between rivers, canals, and paddy fields in driving amphibian invasions, offering critical insights into the conservation and control of invasive species. [ABSTRACT FROM AUTHOR] |
| Copyright of Hydrobiologia is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 192347392 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Multi-taxon environmental DNA analysis reveals distributions and pathways of Pelophylax nigromaculatus invasion in Iwamizawa, Hokkaido, Japan. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Nakashima%2C+Naohisa%22">Nakashima, Naohisa</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> nnakashima@obihiro.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Fukuda%2C+Shinji%22">Fukuda, Shinji</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> shinji-f@cc.tuat.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Matsubara%2C+Hinata%22">Matsubara, Hinata</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ogawa%2C+Kosei%22">Ogawa, Kosei</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Koshiyama%2C+Naoko%22">Koshiyama, Naoko</searchLink><relatesTo>5</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Hydrobiologia%22">Hydrobiologia</searchLink>. Apr2026, Vol. 853 Issue 7, p2075-2091. 17p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Dispersal+%28Ecology%29%22">Dispersal (Ecology)</searchLink><br /><searchLink fieldCode="DE" term="%22Ditches%22">Ditches</searchLink><br /><searchLink fieldCode="DE" term="%22Introduced+species%22">Introduced species</searchLink><br /><searchLink fieldCode="DE" term="%22Amphibian+conservation%22">Amphibian conservation</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acids%22">Nucleic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Habitat+conservation%22">Habitat conservation</searchLink><br /><searchLink fieldCode="DE" term="%22Frogs%22">Frogs</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Hokkaido+%28Japan%29%22">Hokkaido (Japan)</searchLink><br /><searchLink fieldCode="DE" term="%22Japan%22">Japan</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: This study explores the recent expansion of the translocated invasive Black-spotted pond frog Pelophylax nigromaculatus in Iwamizawa, Hokkaido, Japan, focusing on dispersal routes and habitat uses at landscape and field scales. Environmental DNA (eDNA) surveys across rivers and agricultural canals confirmed the occurrence of P. nigromaculatus in Iwamizawa for the first time, and revealed that P. nigromaculatus primarily dispersed via agricultural canals connected to paddy fields rather than larger natural rivers. Smaller canals facilitated species movement, whereas rivers posed potential barriers. Detailed eDNA analysis and visual observations in two adjacent paddy lots—one with an open-ditch system and the other without—demonstrated that frogs likely entered from the irrigation canals. The open-ditch lot supported a population due to continuous inundation even during midseason drainage, whereas reduced water levels in the conventional paddy lot restricted long-term microhabitat use. In contrast to the invasive species, the native Japanese tree frog Dryophytes japonicus was not detected by eDNA in the open-ditch lot, reflecting a habitat segregation of the species. These findings support the important role of agricultural canal networks and connectivity between rivers, canals, and paddy fields in driving amphibian invasions, offering critical insights into the conservation and control of invasive species. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Hydrobiologia is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10750-025-06053-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 17 StartPage: 2075 Subjects: – SubjectFull: Dispersal (Ecology) Type: general – SubjectFull: Ditches Type: general – SubjectFull: Introduced species Type: general – SubjectFull: Amphibian conservation Type: general – SubjectFull: Nucleic acids Type: general – SubjectFull: Habitat conservation Type: general – SubjectFull: Frogs Type: general – SubjectFull: Hokkaido (Japan) Type: general – SubjectFull: Japan Type: general Titles: – TitleFull: Multi-taxon environmental DNA analysis reveals distributions and pathways of Pelophylax nigromaculatus invasion in Iwamizawa, Hokkaido, Japan. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nakashima, Naohisa – PersonEntity: Name: NameFull: Fukuda, Shinji – PersonEntity: Name: NameFull: Matsubara, Hinata – PersonEntity: Name: NameFull: Ogawa, Kosei – PersonEntity: Name: NameFull: Koshiyama, Naoko IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 00188158 Numbering: – Type: volume Value: 853 – Type: issue Value: 7 Titles: – TitleFull: Hydrobiologia Type: main |
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