View in EDS

Role of evaporative light scattering detectors in HPLC and UPLC—a comprehensive review of operating principle and method development principles.

Saved in:
Bibliographic Details
Title: Role of evaporative light scattering detectors in HPLC and UPLC—a comprehensive review of operating principle and method development principles.
Authors: Kuppan, Chandrasekar1 (AUTHOR) ramachan16@gmail.com, Lakka, Narasimha S.1,2 (AUTHOR), Vadagam, Niroja3 (AUTHOR) nvadagam@gitam.in
Source: Journal of Liquid Chromatography & Related Technologies. Jan-Mar2026, Vol. 49 Issue 1-4, p35-49. 15p.
Subjects: Chromatographic detectors, High performance liquid chromatography, Chromatographic analysis, Scientific method, Analytical chemistry, Chemical detectors
Abstract: Evaporative light scattering detectors (ELSD) are widely employed in modern liquid chromatography for detecting organic compounds, offering significant advantages across diverse fields such as pharmaceuticals, biopharmaceuticals, food and beverage analysis, environmental monitoring, and proteomics. As a universal detector, ELSD has become indispensable in modern chromatography, enabling analyte detection independent of chromophores, fluorescence, or electro-active groups. This review highlights the fundamental principles of ELSD operation and provides strategies for method development in both HPLC and UHPLC systems. Key chromatographic parameters—including mobile phase composition, gradient design, stationary phase choice, and column oven temperature—are discussed in relation to ELSD integration. Critical operational aspects such as impactor mode, nebulizer gas flow and temperature, gain settings, evaporator or drift tube temperatures, and solvent flow rates are examined in detail. The influence of sample load on peak symmetry and the role of nebulization in spike formation are also evaluated. Furthermore, ELSD compatibility with preparative liquid chromatography and ion chromatography is explored, alongside practical applications. Comparative performance with conventional detectors, including UV-Vis, refractive index, fluorescence, and advanced techniques such as mass spectrometry and charged aerosol detection, is assessed. Collectively, this framework supports optimization of ELSD-based methodologies across a broad spectrum of analytical applications. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Liquid Chromatography & Related Technologies is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 192434219
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Role of evaporative light scattering detectors in HPLC and UPLC—a comprehensive review of operating principle and method development principles.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Kuppan%2C+Chandrasekar%22">Kuppan, Chandrasekar</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ramachan16@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Lakka%2C+Narasimha+S%2E%22">Lakka, Narasimha S.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Vadagam%2C+Niroja%22">Vadagam, Niroja</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> nvadagam@gitam.in</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Liquid+Chromatography+%26+Related+Technologies%22">Journal of Liquid Chromatography & Related Technologies</searchLink>. Jan-Mar2026, Vol. 49 Issue 1-4, p35-49. 15p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Chromatographic+detectors%22">Chromatographic detectors</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Scientific+method%22">Scientific method</searchLink><br /><searchLink fieldCode="DE" term="%22Analytical+chemistry%22">Analytical chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+detectors%22">Chemical detectors</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Evaporative light scattering detectors (ELSD) are widely employed in modern liquid chromatography for detecting organic compounds, offering significant advantages across diverse fields such as pharmaceuticals, biopharmaceuticals, food and beverage analysis, environmental monitoring, and proteomics. As a universal detector, ELSD has become indispensable in modern chromatography, enabling analyte detection independent of chromophores, fluorescence, or electro-active groups. This review highlights the fundamental principles of ELSD operation and provides strategies for method development in both HPLC and UHPLC systems. Key chromatographic parameters—including mobile phase composition, gradient design, stationary phase choice, and column oven temperature—are discussed in relation to ELSD integration. Critical operational aspects such as impactor mode, nebulizer gas flow and temperature, gain settings, evaporator or drift tube temperatures, and solvent flow rates are examined in detail. The influence of sample load on peak symmetry and the role of nebulization in spike formation are also evaluated. Furthermore, ELSD compatibility with preparative liquid chromatography and ion chromatography is explored, alongside practical applications. Comparative performance with conventional detectors, including UV-Vis, refractive index, fluorescence, and advanced techniques such as mass spectrometry and charged aerosol detection, is assessed. Collectively, this framework supports optimization of ELSD-based methodologies across a broad spectrum of analytical applications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Liquid Chromatography & Related Technologies is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=192434219
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1080/10826076.2025.2602140
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 15
        StartPage: 35
    Subjects:
      – SubjectFull: Chromatographic detectors
        Type: general
      – SubjectFull: High performance liquid chromatography
        Type: general
      – SubjectFull: Chromatographic analysis
        Type: general
      – SubjectFull: Scientific method
        Type: general
      – SubjectFull: Analytical chemistry
        Type: general
      – SubjectFull: Chemical detectors
        Type: general
    Titles:
      – TitleFull: Role of evaporative light scattering detectors in HPLC and UPLC—a comprehensive review of operating principle and method development principles.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Kuppan, Chandrasekar
      – PersonEntity:
          Name:
            NameFull: Lakka, Narasimha S.
      – PersonEntity:
          Name:
            NameFull: Vadagam, Niroja
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Text: Jan-Mar2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 10826076
          Numbering:
            – Type: volume
              Value: 49
            – Type: issue
              Value: 1-4
          Titles:
            – TitleFull: Journal of Liquid Chromatography & Related Technologies
              Type: main
ResultId 1