Evaluation of the biological activities of Bacillus cereus-synthesized titanium dioxide nanoparticles.
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| Title: | Evaluation of the biological activities of Bacillus cereus-synthesized titanium dioxide nanoparticles. |
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| Authors: | Abdallah, Marwa M. A.1 (AUTHOR) Marwa.abdelatif@bue.edu.eg, El-Kased, Reham F.1 (AUTHOR) Reham.kased@bue.edu.eg, El-Housseiny, Ghadir S.2 (AUTHOR) ghadir.elhossaieny@pharma.asu.edu.eg, Aboshanab, Khaled M.2 (AUTHOR) aboshanab2012@pharma.asu.edu.eg |
| Source: | Applied Microbiology & Biotechnology. 3/23/2026, Vol. 110 Issue 1, p1-20. 20p. |
| Subjects: | Titanium dioxide nanoparticles, Bacillus cereus, Antiviral agents, Biofilms, Antineoplastic agents, Microbiological synthesis, Anti-infective agents |
| Abstract: | The emergence of multidrug-resistant pathogens and the growing burden of cancer worldwide demand novel therapeutic approaches. This study evaluated the biological activities of green-synthesized titanium dioxide nanoparticles (TiO₂ NPs) using Bacillus cereus cell-free supernatant and titanium tetra-isopropoxide precursor. Nanoparticles were analyzed and standard methods were employed to evaluate the antimicrobial, antibiofilm, and cell cytotoxicity. UV–Vis spectroscopy confirmed the synthesis of TiO₂ NPs with a characteristic peak at 360 nm. Transmission electron microscopy (TEM) revealed roughly spherical particles (8.8 ± 3 nm diameter). A Z-average hydrodynamic diameter of 328.1 nm (PDI, 0.439) was revealed by dynamic light scattering (DLS). According to the energy-dispersive X-ray spectroscopy (EDX) results, oxygen was 42.39% and titanium was 45.93%. The X-ray diffraction (XRD) measurement confirmed a crystalline anatase structure, with a prominent peak at 25.3° 2θ. Fourier transform infrared (FTIR) detected the characteristic Ti–O-Ti and vibrational band at 403.31 cm⁻1. The nanoparticles demonstrated broad-spectrum antimicrobial activity with MICs of 78 μg/mL (E. coli), 156 μg/mL (C. albicans), 625 μg/mL (E. faecalis), and 2500 μg/mL (S. aureus and P. aeruginosa). At sub-MIC concentrations, TiO₂ NPs reduced biofilm formation in S. aureus by roughly 65.72% ± 0.01, in E. faecalis by 50.91% ± 0.02, and in P. aeruginosa by 38.4% ± 0.1. Cytotoxicity investigations revealed IC₅₀ values of 212.15 μg/mL (Caco-2) and 342.96 μg/mL (Vero cells), with a selectivity index of 1.62. Strong antiviral activity against HSV-1 was observed (74.74% inhibition at 60 µg/mL, EC₅₀ = 17.41 µg/mL, selectivity index = 19.7). Biogenic synthesis using B. cereus successfully produced highly crystalline, small-sized anatase TiO₂ nanoparticles demonstrating multifunctional biological activities including antimicrobial, antibiofilm, anticancer selectivity, and antiviral properties. Key points: • Green synthesis via B. cereus yielded crystalline anatase TiO₂ NPs with nanoscale dimensions. • TiO₂ NPs demonstrated broad antimicrobial/antibiofilm effects and selective anticancer activity. • Strong antiviral potency against HSV-1 with excellent selectivity was observed [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 192461641 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Evaluation of the biological activities of Bacillus cereus-synthesized titanium dioxide nanoparticles. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Abdallah%2C+Marwa+M%2E+A%2E%22">Abdallah, Marwa M. A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> Marwa.abdelatif@bue.edu.eg</i><br /><searchLink fieldCode="AR" term="%22El-Kased%2C+Reham+F%2E%22">El-Kased, Reham F.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> Reham.kased@bue.edu.eg</i><br /><searchLink fieldCode="AR" term="%22El-Housseiny%2C+Ghadir+S%2E%22">El-Housseiny, Ghadir S.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> ghadir.elhossaieny@pharma.asu.edu.eg</i><br /><searchLink fieldCode="AR" term="%22Aboshanab%2C+Khaled+M%2E%22">Aboshanab, Khaled M.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> aboshanab2012@pharma.asu.edu.eg</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. 3/23/2026, Vol. 110 Issue 1, p1-20. 20p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Titanium+dioxide+nanoparticles%22">Titanium dioxide nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Bacillus+cereus%22">Bacillus cereus</searchLink><br /><searchLink fieldCode="DE" term="%22Antiviral+agents%22">Antiviral agents</searchLink><br /><searchLink fieldCode="DE" term="%22Biofilms%22">Biofilms</searchLink><br /><searchLink fieldCode="DE" term="%22Antineoplastic+agents%22">Antineoplastic agents</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiological+synthesis%22">Microbiological synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Anti-infective+agents%22">Anti-infective agents</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The emergence of multidrug-resistant pathogens and the growing burden of cancer worldwide demand novel therapeutic approaches. This study evaluated the biological activities of green-synthesized titanium dioxide nanoparticles (TiO₂ NPs) using Bacillus cereus cell-free supernatant and titanium tetra-isopropoxide precursor. Nanoparticles were analyzed and standard methods were employed to evaluate the antimicrobial, antibiofilm, and cell cytotoxicity. UV–Vis spectroscopy confirmed the synthesis of TiO₂ NPs with a characteristic peak at 360 nm. Transmission electron microscopy (TEM) revealed roughly spherical particles (8.8 ± 3 nm diameter). A Z-average hydrodynamic diameter of 328.1 nm (PDI, 0.439) was revealed by dynamic light scattering (DLS). According to the energy-dispersive X-ray spectroscopy (EDX) results, oxygen was 42.39% and titanium was 45.93%. The X-ray diffraction (XRD) measurement confirmed a crystalline anatase structure, with a prominent peak at 25.3° 2θ. Fourier transform infrared (FTIR) detected the characteristic Ti–O-Ti and vibrational band at 403.31 cm⁻1. The nanoparticles demonstrated broad-spectrum antimicrobial activity with MICs of 78 μg/mL (E. coli), 156 μg/mL (C. albicans), 625 μg/mL (E. faecalis), and 2500 μg/mL (S. aureus and P. aeruginosa). At sub-MIC concentrations, TiO₂ NPs reduced biofilm formation in S. aureus by roughly 65.72% ± 0.01, in E. faecalis by 50.91% ± 0.02, and in P. aeruginosa by 38.4% ± 0.1. Cytotoxicity investigations revealed IC₅₀ values of 212.15 μg/mL (Caco-2) and 342.96 μg/mL (Vero cells), with a selectivity index of 1.62. Strong antiviral activity against HSV-1 was observed (74.74% inhibition at 60 µg/mL, EC₅₀ = 17.41 µg/mL, selectivity index = 19.7). Biogenic synthesis using B. cereus successfully produced highly crystalline, small-sized anatase TiO₂ nanoparticles demonstrating multifunctional biological activities including antimicrobial, antibiofilm, anticancer selectivity, and antiviral properties. Key points: • Green synthesis via B. cereus yielded crystalline anatase TiO₂ NPs with nanoscale dimensions. • TiO₂ NPs demonstrated broad antimicrobial/antibiofilm effects and selective anticancer activity. • Strong antiviral potency against HSV-1 with excellent selectivity was observed [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-026-13771-8 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 20 StartPage: 1 Subjects: – SubjectFull: Titanium dioxide nanoparticles Type: general – SubjectFull: Bacillus cereus Type: general – SubjectFull: Antiviral agents Type: general – SubjectFull: Biofilms Type: general – SubjectFull: Antineoplastic agents Type: general – SubjectFull: Microbiological synthesis Type: general – SubjectFull: Anti-infective agents Type: general Titles: – TitleFull: Evaluation of the biological activities of Bacillus cereus-synthesized titanium dioxide nanoparticles. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Abdallah, Marwa M. A. – PersonEntity: Name: NameFull: El-Kased, Reham F. – PersonEntity: Name: NameFull: El-Housseiny, Ghadir S. – PersonEntity: Name: NameFull: Aboshanab, Khaled M. IsPartOfRelationships: – BibEntity: Dates: – D: 23 M: 03 Text: 3/23/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 110 – Type: issue Value: 1 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
