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Sulfide-driven mixotrophic partial denitrification: From performance to microbial mechanism.

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Title: Sulfide-driven mixotrophic partial denitrification: From performance to microbial mechanism.
Authors: Chen, Zebin1 (AUTHOR), Kang, Peilun1 (AUTHOR), Ye, Shengqi1 (AUTHOR), Xie, Bing1 (AUTHOR), Xie, Xinyu1 (AUTHOR), Song, Jingwen1 (AUTHOR), Ma, Yiting1 (AUTHOR), Wu, Zixuan1 (AUTHOR), Cui, Xi1 (AUTHOR), Zhang, Xiaojing2 (AUTHOR), Yu, Guangwei1 (AUTHOR), Liang, Yuhai1 (AUTHOR) liangyuhai@scau.edu.cn
Source: Chemical Engineering Journal. Apr2026, Vol. 534, pN.PAG-N.PAG. 1p.
Subjects: Denitrification, Nitrites, Nitrate reductase, Genes, Bacteria, Bacterial adaptation, Sludge management
Abstract: Partial denitrification can provide a continuous supply of nitrite for anammox. To circumvent the bottlenecks of microbial competition and inhibition inherent in integrated one-stage systems, a mixotrophic partial denitrification (S-MPD) system driven by sulfide and acetate was constructed in an independent reactor to achieve decoupled operation from anammox. The regulatory effects of S/C/N ratios, sludge discharge, and long-term acclimation on system performance and microbial mechanisms were investigated. The results demonstrated that at a S/C/N ratio of 0.24:1.31:1, the nitrate removal efficiency reached (97.8 ± 1.1)% with a nitrite accumulation rate of (81.4 ± 2.3)%. When the S/C/N ratio was increased to 0.48:0.88:1, the system recovered stable operation through sludge discharge and long-term acclimation, maintaining a nitrite accumulation rate of (68.6 ± 3.6)%. Microbial analysis revealed that Thauera was the key mixotrophic denitrifier responsible for S-MPD. By dynamically regulating the abundances of narG and soxB genes, Thauera mitigated sulfur stress and optimized electron flow distribution, thereby maintaining efficient nitrite accumulation. This study confirms the stability and regulatory potential of decoupled S-MPD for efficient nitrite accumulation, providing a theoretical basis for the construction and optimization of two-stage S-MPD/anammox processes. [Display omitted] • The independent S-MPD reactor achieved stable nitrite accumulation with mixed electron donors. • Sludge discharge and long-term acclimation restored S-MPD performance after sulfide inhibition. • Thauera emerged as the key bacterium by regulating narG and soxB genes under sulfide stress. [ABSTRACT FROM AUTHOR]
Copyright of Chemical Engineering Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 192789519
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PubTypeId: academicJournal
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  Data: Sulfide-driven mixotrophic partial denitrification: From performance to microbial mechanism.
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  Data: <searchLink fieldCode="AR" term="%22Chen%2C+Zebin%22">Chen, Zebin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kang%2C+Peilun%22">Kang, Peilun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ye%2C+Shengqi%22">Ye, Shengqi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xie%2C+Bing%22">Xie, Bing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xie%2C+Xinyu%22">Xie, Xinyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Song%2C+Jingwen%22">Song, Jingwen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ma%2C+Yiting%22">Ma, Yiting</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wu%2C+Zixuan%22">Wu, Zixuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cui%2C+Xi%22">Cui, Xi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Xiaojing%22">Zhang, Xiaojing</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yu%2C+Guangwei%22">Yu, Guangwei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liang%2C+Yuhai%22">Liang, Yuhai</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> liangyuhai@scau.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Chemical+Engineering+Journal%22">Chemical Engineering Journal</searchLink>. Apr2026, Vol. 534, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Denitrification%22">Denitrification</searchLink><br /><searchLink fieldCode="DE" term="%22Nitrites%22">Nitrites</searchLink><br /><searchLink fieldCode="DE" term="%22Nitrate+reductase%22">Nitrate reductase</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteria%22">Bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+adaptation%22">Bacterial adaptation</searchLink><br /><searchLink fieldCode="DE" term="%22Sludge+management%22">Sludge management</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Partial denitrification can provide a continuous supply of nitrite for anammox. To circumvent the bottlenecks of microbial competition and inhibition inherent in integrated one-stage systems, a mixotrophic partial denitrification (S-MPD) system driven by sulfide and acetate was constructed in an independent reactor to achieve decoupled operation from anammox. The regulatory effects of S/C/N ratios, sludge discharge, and long-term acclimation on system performance and microbial mechanisms were investigated. The results demonstrated that at a S/C/N ratio of 0.24:1.31:1, the nitrate removal efficiency reached (97.8 ± 1.1)% with a nitrite accumulation rate of (81.4 ± 2.3)%. When the S/C/N ratio was increased to 0.48:0.88:1, the system recovered stable operation through sludge discharge and long-term acclimation, maintaining a nitrite accumulation rate of (68.6 ± 3.6)%. Microbial analysis revealed that Thauera was the key mixotrophic denitrifier responsible for S-MPD. By dynamically regulating the abundances of narG and soxB genes, Thauera mitigated sulfur stress and optimized electron flow distribution, thereby maintaining efficient nitrite accumulation. This study confirms the stability and regulatory potential of decoupled S-MPD for efficient nitrite accumulation, providing a theoretical basis for the construction and optimization of two-stage S-MPD/anammox processes. [Display omitted] • The independent S-MPD reactor achieved stable nitrite accumulation with mixed electron donors. • Sludge discharge and long-term acclimation restored S-MPD performance after sulfide inhibition. • Thauera emerged as the key bacterium by regulating narG and soxB genes under sulfide stress. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Chemical Engineering Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.cej.2026.175104
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Denitrification
        Type: general
      – SubjectFull: Nitrites
        Type: general
      – SubjectFull: Nitrate reductase
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      – SubjectFull: Genes
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      – SubjectFull: Bacteria
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      – SubjectFull: Bacterial adaptation
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      – SubjectFull: Sludge management
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              Text: Apr2026
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