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Development and Validation of an HPLC Method for Determination of the Accumulation Patterns of Coumarin and 2-Hydroxycinnamic Acid in Different Parts of Eupatorium Fortunei Turcz. During Growth Stages.

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Title: Development and Validation of an HPLC Method for Determination of the Accumulation Patterns of Coumarin and 2-Hydroxycinnamic Acid in Different Parts of Eupatorium Fortunei Turcz. During Growth Stages.
Authors: Zheng, Shaohua1 (AUTHOR), Zhang, Lina2 (AUTHOR), Nan, Guanjun2 (AUTHOR), Yang, Guangde2 (AUTHOR), Shi, Bowen3 (AUTHOR)
Source: Journal of Chromatographic Science. Mar2026, Vol. 64 Issue 3, p1-7. 7p.
Subjects: Coumarins, Hydroxycinnamic acids, Quality control, Metabolites, Medicinal plants, High performance liquid chromatography, Plant life cycles, Eupatorium
Abstract: To quantify coumarin and 2-hydroxycinnamic acid in Eupatorium fortune Turcz. (EFT), a high-performance liquid chromatography method was developed and rigorously validated. This optimized approach was subsequently applied to analyze the distribution patterns of both compounds across different plant tissues and at various growth stages. The results indicated that the contents of coumarin and 2-hydroxycinnamic acid varied significantly among plant parts and over time. Specifically, the concentrations in leaves substantially exceeded those in stems and roots, establishing the following concentration gradient: leaves > stems > roots. Throughout the growth cycle, the levels of both compounds exhibited two distinct peaks amid a general declining trend. Based on these accumulation dynamics, two harvest periods are recommended within one growth cycle: the optimal harvest time occurs in early May, with a secondary period in early July. Furthermore, the leaves were identified as the most promising medicinal tissue due to their markedly higher bioactive content. These findings offer valuable insights for improving the quality control and agricultural practices related to EFT. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chromatographic Science is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 192796059
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PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: Development and Validation of an HPLC Method for Determination of the Accumulation Patterns of Coumarin and 2-Hydroxycinnamic Acid in Different Parts of Eupatorium Fortunei Turcz. During Growth Stages.
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatographic+Science%22">Journal of Chromatographic Science</searchLink>. Mar2026, Vol. 64 Issue 3, p1-7. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Coumarins%22">Coumarins</searchLink><br /><searchLink fieldCode="DE" term="%22Hydroxycinnamic+acids%22">Hydroxycinnamic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Quality+control%22">Quality control</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolites%22">Metabolites</searchLink><br /><searchLink fieldCode="DE" term="%22Medicinal+plants%22">Medicinal plants</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+life+cycles%22">Plant life cycles</searchLink><br /><searchLink fieldCode="DE" term="%22Eupatorium%22">Eupatorium</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: To quantify coumarin and 2-hydroxycinnamic acid in Eupatorium fortune Turcz. (EFT), a high-performance liquid chromatography method was developed and rigorously validated. This optimized approach was subsequently applied to analyze the distribution patterns of both compounds across different plant tissues and at various growth stages. The results indicated that the contents of coumarin and 2-hydroxycinnamic acid varied significantly among plant parts and over time. Specifically, the concentrations in leaves substantially exceeded those in stems and roots, establishing the following concentration gradient: leaves > stems > roots. Throughout the growth cycle, the levels of both compounds exhibited two distinct peaks amid a general declining trend. Based on these accumulation dynamics, two harvest periods are recommended within one growth cycle: the optimal harvest time occurs in early May, with a secondary period in early July. Furthermore, the leaves were identified as the most promising medicinal tissue due to their markedly higher bioactive content. These findings offer valuable insights for improving the quality control and agricultural practices related to EFT. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatographic Science is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1093/chromsci/bmag001
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 7
        StartPage: 1
    Subjects:
      – SubjectFull: Coumarins
        Type: general
      – SubjectFull: Hydroxycinnamic acids
        Type: general
      – SubjectFull: Quality control
        Type: general
      – SubjectFull: Metabolites
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      – SubjectFull: Medicinal plants
        Type: general
      – SubjectFull: High performance liquid chromatography
        Type: general
      – SubjectFull: Plant life cycles
        Type: general
      – SubjectFull: Eupatorium
        Type: general
    Titles:
      – TitleFull: Development and Validation of an HPLC Method for Determination of the Accumulation Patterns of Coumarin and 2-Hydroxycinnamic Acid in Different Parts of Eupatorium Fortunei Turcz. During Growth Stages.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Zheng, Shaohua
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          Name:
            NameFull: Zhang, Lina
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            NameFull: Nan, Guanjun
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            NameFull: Yang, Guangde
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            NameFull: Shi, Bowen
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            – D: 01
              M: 03
              Text: Mar2026
              Type: published
              Y: 2026
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              Value: 64
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            – TitleFull: Journal of Chromatographic Science
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