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Effect of Fullerenol C 60 (OH) 24 on Viability and Phagocytic Activity of Human Neutrophils.

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Title: Effect of Fullerenol C 60 (OH) 24 on Viability and Phagocytic Activity of Human Neutrophils.
Authors: Lazarev, Sergey1 (AUTHOR), Timganova, Valeria1,2 (AUTHOR), Bochkova, Maria1,2 (AUTHOR), Dolgikh, Maria1,2 (AUTHOR), Usanina, Darya1,2 (AUTHOR), Zamorina, Svetlana1,2 (AUTHOR), Rayev, Mikhail1,2 (AUTHOR) mraev@iegm.ru
Source: Nanomaterials (2079-4991). Apr2026, Vol. 16 Issue 7, p405. 20p.
Subjects: Neutrophils, Fullerene derivatives, Reactive oxygen species, Cell death, Nanomedicine, Cell survival, Antioxidants, Phagocytosis
Abstract: Water-soluble fullerene derivatives such as fullerenol C60(OH)24 are promising candidates for nanomedicine applications, yet their effects on innate immune cells remain poorly characterized. We investigated the interaction of fullerenol with human neutrophils isolated from healthy donors, exposed to concentrations of 0.25–200 μg/mL over 24–72 h. Using multi-parameter flow cytometry, we assessed viability, apoptosis, phagocytic activity, and intracellular reactive oxygen species (ROS) production, complemented by cell-free DPPH radical scavenging assays. Fullerenol was taken up by neutrophils in a concentration- and time-dependent manner. No significant cytotoxicity was observed up to 100 μg/mL, while viability declined at 200 μg/mL. Phagocytosis of opsonized E. coli was preserved at lower concentrations, though a statistically significant negative correlation with fullerenol concentration was detected at higher doses. In cell-free assays, fullerenol scavenged DPPH radicals with an EC50 of 48.90 ± 10.02 μg/mL, exhibiting slower kinetics than Trolox or ascorbic acid. Critically, fullerenol suppressed intracellular ROS production by >33% at 50 μg/mL following PMA stimulation of neutrophils. These findings demonstrate that fullerenol C60(OH)24 combines potent intracellular antioxidant activity with a favorable neutrophil safety profile, supporting its potential application in oxidative stress-related conditions. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
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  Data: Effect of Fullerenol C 60 (OH) 24 on Viability and Phagocytic Activity of Human Neutrophils.
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  Data: <searchLink fieldCode="AR" term="%22Lazarev%2C+Sergey%22">Lazarev, Sergey</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Timganova%2C+Valeria%22">Timganova, Valeria</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bochkova%2C+Maria%22">Bochkova, Maria</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dolgikh%2C+Maria%22">Dolgikh, Maria</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Usanina%2C+Darya%22">Usanina, Darya</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zamorina%2C+Svetlana%22">Zamorina, Svetlana</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rayev%2C+Mikhail%22">Rayev, Mikhail</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> mraev@iegm.ru</i>
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Apr2026, Vol. 16 Issue 7, p405. 20p.
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  Data: <searchLink fieldCode="DE" term="%22Neutrophils%22">Neutrophils</searchLink><br /><searchLink fieldCode="DE" term="%22Fullerene+derivatives%22">Fullerene derivatives</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+death%22">Cell death</searchLink><br /><searchLink fieldCode="DE" term="%22Nanomedicine%22">Nanomedicine</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+survival%22">Cell survival</searchLink><br /><searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Phagocytosis%22">Phagocytosis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Water-soluble fullerene derivatives such as fullerenol C60(OH)24 are promising candidates for nanomedicine applications, yet their effects on innate immune cells remain poorly characterized. We investigated the interaction of fullerenol with human neutrophils isolated from healthy donors, exposed to concentrations of 0.25–200 μg/mL over 24–72 h. Using multi-parameter flow cytometry, we assessed viability, apoptosis, phagocytic activity, and intracellular reactive oxygen species (ROS) production, complemented by cell-free DPPH radical scavenging assays. Fullerenol was taken up by neutrophils in a concentration- and time-dependent manner. No significant cytotoxicity was observed up to 100 μg/mL, while viability declined at 200 μg/mL. Phagocytosis of opsonized E. coli was preserved at lower concentrations, though a statistically significant negative correlation with fullerenol concentration was detected at higher doses. In cell-free assays, fullerenol scavenged DPPH radicals with an EC50 of 48.90 ± 10.02 μg/mL, exhibiting slower kinetics than Trolox or ascorbic acid. Critically, fullerenol suppressed intracellular ROS production by >33% at 50 μg/mL following PMA stimulation of neutrophils. These findings demonstrate that fullerenol C60(OH)24 combines potent intracellular antioxidant activity with a favorable neutrophil safety profile, supporting its potential application in oxidative stress-related conditions. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/nano16070405
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        Text: English
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        PageCount: 20
        StartPage: 405
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      – SubjectFull: Neutrophils
        Type: general
      – SubjectFull: Fullerene derivatives
        Type: general
      – SubjectFull: Reactive oxygen species
        Type: general
      – SubjectFull: Cell death
        Type: general
      – SubjectFull: Nanomedicine
        Type: general
      – SubjectFull: Cell survival
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      – SubjectFull: Antioxidants
        Type: general
      – SubjectFull: Phagocytosis
        Type: general
    Titles:
      – TitleFull: Effect of Fullerenol C 60 (OH) 24 on Viability and Phagocytic Activity of Human Neutrophils.
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              M: 04
              Text: Apr2026
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              Y: 2026
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