Optical label-free detection of SARS-CoV-2: investigating platform spectroscopic properties for oligonucleotide targeting.
Saved in:
| Title: | Optical label-free detection of SARS-CoV-2: investigating platform spectroscopic properties for oligonucleotide targeting. |
|---|---|
| Authors: | Rotondi, Silvia Maria Cristina1 (AUTHOR), Canepa, Paolo1 (AUTHOR) paolocanepa@unige.it, Dante, Silvia2 (AUTHOR), Canepa, Maurizio1 (AUTHOR), Cavalleri, Ornella1 (AUTHOR) ornella.cavalleri@unige.it |
| Source: | European Biophysics Journal. Apr2026, Vol. 55 Issue 2, p209-216. 8p. |
| Subjects: | Oligonucleotides, Ellipsometry, Selectivity (Psychology), Viral genomes, Optical signal detection, COVID-19 testing, Binding constant, Biosensors |
| Abstract: | We characterized a DNA/gold interface designed for the detection of the SARS-CoV-2 RNA-dependent RNA polymerase/Helicase (RdRp/Hel) sequence. Using broadband spectroscopic ellipsometry (SE) and a difference spectra approach, we monitored molecular modifications at the interface, from probe sequence deposition to the insertion of a molecular spacer and subsequent hybridization with the target. The UV region revealed the characteristic DNA absorption peak around 260 nm, while changes in δΔ in the NIR correlated with increased optical thickness following each deposition step. The optical response was analyzed as a function of target concentration, and the binding affinity curve, derived from δΔ values at 800 nm, was fitted using a first-order Langmuir model, yielding a dissociation constant KD = (70 ± 10) nM, consistent with literature values. Selectivity studies demonstrated that the interface effectively discriminates the SARS-CoV-2 sequence from the SARS-CoV HKU variant, even in a crowded environment. A complementary platform targeting the SARS-CoV HKU sequence confirmed selective detection of HKU over SARS-CoV-2. These findings highlight the potential for parallel detection of different viral sequences. [ABSTRACT FROM AUTHOR] |
| Copyright of European Biophysics Journal is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 193278284 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Optical label-free detection of SARS-CoV-2: investigating platform spectroscopic properties for oligonucleotide targeting. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Rotondi%2C+Silvia+Maria+Cristina%22">Rotondi, Silvia Maria Cristina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Canepa%2C+Paolo%22">Canepa, Paolo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> paolocanepa@unige.it</i><br /><searchLink fieldCode="AR" term="%22Dante%2C+Silvia%22">Dante, Silvia</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Canepa%2C+Maurizio%22">Canepa, Maurizio</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cavalleri%2C+Ornella%22">Cavalleri, Ornella</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ornella.cavalleri@unige.it</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22European+Biophysics+Journal%22">European Biophysics Journal</searchLink>. Apr2026, Vol. 55 Issue 2, p209-216. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Oligonucleotides%22">Oligonucleotides</searchLink><br /><searchLink fieldCode="DE" term="%22Ellipsometry%22">Ellipsometry</searchLink><br /><searchLink fieldCode="DE" term="%22Selectivity+%28Psychology%29%22">Selectivity (Psychology)</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+genomes%22">Viral genomes</searchLink><br /><searchLink fieldCode="DE" term="%22Optical+signal+detection%22">Optical signal detection</searchLink><br /><searchLink fieldCode="DE" term="%22COVID-19+testing%22">COVID-19 testing</searchLink><br /><searchLink fieldCode="DE" term="%22Binding+constant%22">Binding constant</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: We characterized a DNA/gold interface designed for the detection of the SARS-CoV-2 RNA-dependent RNA polymerase/Helicase (RdRp/Hel) sequence. Using broadband spectroscopic ellipsometry (SE) and a difference spectra approach, we monitored molecular modifications at the interface, from probe sequence deposition to the insertion of a molecular spacer and subsequent hybridization with the target. The UV region revealed the characteristic DNA absorption peak around 260 nm, while changes in δΔ in the NIR correlated with increased optical thickness following each deposition step. The optical response was analyzed as a function of target concentration, and the binding affinity curve, derived from δΔ values at 800 nm, was fitted using a first-order Langmuir model, yielding a dissociation constant KD = (70 ± 10) nM, consistent with literature values. Selectivity studies demonstrated that the interface effectively discriminates the SARS-CoV-2 sequence from the SARS-CoV HKU variant, even in a crowded environment. A complementary platform targeting the SARS-CoV HKU sequence confirmed selective detection of HKU over SARS-CoV-2. These findings highlight the potential for parallel detection of different viral sequences. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of European Biophysics Journal is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=193278284 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00249-025-01787-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 209 Subjects: – SubjectFull: Oligonucleotides Type: general – SubjectFull: Ellipsometry Type: general – SubjectFull: Selectivity (Psychology) Type: general – SubjectFull: Viral genomes Type: general – SubjectFull: Optical signal detection Type: general – SubjectFull: COVID-19 testing Type: general – SubjectFull: Binding constant Type: general – SubjectFull: Biosensors Type: general Titles: – TitleFull: Optical label-free detection of SARS-CoV-2: investigating platform spectroscopic properties for oligonucleotide targeting. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Rotondi, Silvia Maria Cristina – PersonEntity: Name: NameFull: Canepa, Paolo – PersonEntity: Name: NameFull: Dante, Silvia – PersonEntity: Name: NameFull: Canepa, Maurizio – PersonEntity: Name: NameFull: Cavalleri, Ornella IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 01757571 Numbering: – Type: volume Value: 55 – Type: issue Value: 2 Titles: – TitleFull: European Biophysics Journal Type: main |
| ResultId | 1 |
