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Applications and mechanisms of electrical stimulation in plant in vitro culture and micropropagation.

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Title: Applications and mechanisms of electrical stimulation in plant in vitro culture and micropropagation.
Authors: Zheng, Yuhong1 (AUTHOR), Fu, Li2 (AUTHOR) fuli@hdu.edu.cn
Source: Plant Biotechnology Reports. Jun2026, Vol. 20 Issue 3, p1-21. 21p.
Subjects: Plant micropropagation, Plant cell culture, Electronic pulse techniques, Somatic embryogenesis, Biotechnology, Metabolites, Electroporation
Abstract: Electrical stimulation represents an emerging physical biotechnology for enhancing plant in vitro culture and micropropagation, offering potential solutions to persistent challenges including genotype-dependent recalcitrance and suboptimal metabolite yields that limit conventional chemically-based approaches. This review critically synthesizes current knowledge on the applications and mechanisms of electrical stimulation modalities including direct current (DC), alternating current (AC), and pulsed electric fields (PEF), across diverse tissue culture contexts. We examine documented effects on shoot and root organogenesis, somatic embryogenesis, protoplast transformation, and secondary metabolite production, where treatments have achieved 2–5-fold enhancements in regeneration efficiency and significant increases in valuable compounds such as taxanes, camptothecin, and phenolics. The mechanisms proposed to underlie these responses include transient membrane permeabilization under pulsed electric field conditions, electrically induced changes in ion flux and Ca²⁺ signaling, possible alterations in hormone distribution, and ROS-associated stress signaling. However, except for electroporation under appropriate pulse conditions, several of these pathways remain incompletely resolved and may be context dependent. Despite reproducible benefits demonstrated over four decades, the field remains transitional between laboratory proof-of-concept and practical implementation, hindered by critical methodological inconsistencies including lack of standardized electrical parameters, inadequate reporting of electrode configurations, and insufficient mechanistic understanding of how exogenous fields interface with endogenous bioelectric networks. Species- and genotype-specific variability further complicates protocol optimization, while scale-up to commercial bioreactors presents unresolved engineering challenges. Future progress depends on developing international reporting guidelines, conducting rigorous multi-laboratory validation studies, and employing multi-omics approaches to elucidate signaling pathways, ultimately establishing electrical stimulation as a standardized tool for plant biotechnology applications. [ABSTRACT FROM AUTHOR]
Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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  Data: Applications and mechanisms of electrical stimulation in plant in vitro culture and micropropagation.
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  Data: <searchLink fieldCode="AR" term="%22Zheng%2C+Yuhong%22">Zheng, Yuhong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fu%2C+Li%22">Fu, Li</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> fuli@hdu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Plant+Biotechnology+Reports%22">Plant Biotechnology Reports</searchLink>. Jun2026, Vol. 20 Issue 3, p1-21. 21p.
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  Data: <searchLink fieldCode="DE" term="%22Plant+micropropagation%22">Plant micropropagation</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+cell+culture%22">Plant cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22Electronic+pulse+techniques%22">Electronic pulse techniques</searchLink><br /><searchLink fieldCode="DE" term="%22Somatic+embryogenesis%22">Somatic embryogenesis</searchLink><br /><searchLink fieldCode="DE" term="%22Biotechnology%22">Biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolites%22">Metabolites</searchLink><br /><searchLink fieldCode="DE" term="%22Electroporation%22">Electroporation</searchLink>
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  Label: Abstract
  Group: Ab
  Data: Electrical stimulation represents an emerging physical biotechnology for enhancing plant in vitro culture and micropropagation, offering potential solutions to persistent challenges including genotype-dependent recalcitrance and suboptimal metabolite yields that limit conventional chemically-based approaches. This review critically synthesizes current knowledge on the applications and mechanisms of electrical stimulation modalities including direct current (DC), alternating current (AC), and pulsed electric fields (PEF), across diverse tissue culture contexts. We examine documented effects on shoot and root organogenesis, somatic embryogenesis, protoplast transformation, and secondary metabolite production, where treatments have achieved 2–5-fold enhancements in regeneration efficiency and significant increases in valuable compounds such as taxanes, camptothecin, and phenolics. The mechanisms proposed to underlie these responses include transient membrane permeabilization under pulsed electric field conditions, electrically induced changes in ion flux and Ca²⁺ signaling, possible alterations in hormone distribution, and ROS-associated stress signaling. However, except for electroporation under appropriate pulse conditions, several of these pathways remain incompletely resolved and may be context dependent. Despite reproducible benefits demonstrated over four decades, the field remains transitional between laboratory proof-of-concept and practical implementation, hindered by critical methodological inconsistencies including lack of standardized electrical parameters, inadequate reporting of electrode configurations, and insufficient mechanistic understanding of how exogenous fields interface with endogenous bioelectric networks. Species- and genotype-specific variability further complicates protocol optimization, while scale-up to commercial bioreactors presents unresolved engineering challenges. Future progress depends on developing international reporting guidelines, conducting rigorous multi-laboratory validation studies, and employing multi-omics approaches to elucidate signaling pathways, ultimately establishing electrical stimulation as a standardized tool for plant biotechnology applications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=193308499
RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1007/s11816-026-01059-2
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      – Code: eng
        Text: English
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        PageCount: 21
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    Subjects:
      – SubjectFull: Plant micropropagation
        Type: general
      – SubjectFull: Plant cell culture
        Type: general
      – SubjectFull: Electronic pulse techniques
        Type: general
      – SubjectFull: Somatic embryogenesis
        Type: general
      – SubjectFull: Biotechnology
        Type: general
      – SubjectFull: Metabolites
        Type: general
      – SubjectFull: Electroporation
        Type: general
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      – TitleFull: Applications and mechanisms of electrical stimulation in plant in vitro culture and micropropagation.
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            NameFull: Zheng, Yuhong
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            NameFull: Fu, Li
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            – D: 01
              M: 06
              Text: Jun2026
              Type: published
              Y: 2026
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