The adverse effects of paromomycin on early cardiovascular development in zebrafish (Danio rerio).
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| Title: | The adverse effects of paromomycin on early cardiovascular development in zebrafish (Danio rerio). |
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| Authors: | Lin, Yuan1 (AUTHOR), Lei, Yuqing1 (AUTHOR), Zhong, Yijing1 (AUTHOR), Cai, Mingfeng2 (AUTHOR), Wang, Zhenqi2 (AUTHOR), Xie, Haohang2 (AUTHOR), Cao, Hua1 (AUTHOR) caohua69@fjmu.edu.cn, Wang, Xinrui1 (AUTHOR) wanxiru@sjtu.edu.cn |
| Source: | Aquatic Toxicology. Jun2026, Vol. 295, pN.PAG-N.PAG. 1p. |
| Subjects: | Cardiovascular development, Developmental toxicology, Transcriptomes, Toxicology, Oxidative stress, Brachydanio, Molecular docking, Antibiotics |
| Abstract: | • First systematic confirmation of the early cardiovascular developmental toxicity of paromomycin: For the first time, using zebrafish embryos as a model, it was clarified that paromomycin, at environmentally relevant exposure concentrations, can induce cardiovascular developmental damage in early embryos in a dose- and time-dependent manner. • Innovatively integrating network toxicology, molecular docking, and transcriptomics, this study overcomes the limitations of single-method approaches and establishes a comprehensive mechanistic framework from toxic phenotypes to targets and pathways. • It not only clarifies the molecular mechanism of paromomycin-induced cardiovascular toxicity, but also provides direct experimental evidence for the safe use and ecological risk assessment of paromomycin based on the toxicity results at environmentally relevant concentrations, thereby bridging the gap between basic research and practical application. Paromomycin, a broad-spectrum aminoglycoside antibiotic, is widely used in both clinical and agricultural fields. However, its environmental impact and potential developmental toxicity have raised growing concerns. This study systematically evaluates the adverse effects of paromomycin on early cardiovascular development in zebrafish embryos from multiple biological perspectives. Zebrafish embryos were exposed to environmental concentrations of paromomycin (0, 0.3, 3, 30, and 300 μg/mL) for 5 days. Comprehensive assessments were then performed through morphological observations, cardiovascular phenotype analysis, behavioral evaluations, and integrated analysis. The results revealed that exposure to paromomycin induced dose dependent developmental damage, including decreased survival rates, delayed hatching, and increased malformation rates. Detailed cardiovascular assessments confirmed significant abnormalities in both cardiac function and vascular development. Molecular level investigations further elucidated the underlying mechanisms, showing that paromomycin exposure elevated reactive oxygen species levels, triggering oxidative stress, inducing apoptosis in the pericardial region, and disrupting the expression of key cardiovascular developmental genes. Integrating network toxicology, molecular docking, and transcriptomic analyses, this study identified six key signaling pathways involved in paromomycin induced cardiovascular toxicity. These findings provide a comprehensive mechanistic framework for understanding the developmental cardiovascular toxicity of paromomycin at environmentally relevant concentrations and offer valuable insights for the safe application and risk management of this widely used antibiotic. [Display omitted] [ABSTRACT FROM AUTHOR] |
| Copyright of Aquatic Toxicology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 193309449 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: The adverse effects of paromomycin on early cardiovascular development in zebrafish (Danio rerio). – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lin%2C+Yuan%22">Lin, Yuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lei%2C+Yuqing%22">Lei, Yuqing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhong%2C+Yijing%22">Zhong, Yijing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cai%2C+Mingfeng%22">Cai, Mingfeng</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhenqi%22">Wang, Zhenqi</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xie%2C+Haohang%22">Xie, Haohang</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cao%2C+Hua%22">Cao, Hua</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> caohua69@fjmu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Xinrui%22">Wang, Xinrui</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wanxiru@sjtu.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Aquatic+Toxicology%22">Aquatic Toxicology</searchLink>. Jun2026, Vol. 295, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cardiovascular+development%22">Cardiovascular development</searchLink><br /><searchLink fieldCode="DE" term="%22Developmental+toxicology%22">Developmental toxicology</searchLink><br /><searchLink fieldCode="DE" term="%22Transcriptomes%22">Transcriptomes</searchLink><br /><searchLink fieldCode="DE" term="%22Toxicology%22">Toxicology</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidative+stress%22">Oxidative stress</searchLink><br /><searchLink fieldCode="DE" term="%22Brachydanio%22">Brachydanio</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+docking%22">Molecular docking</searchLink><br /><searchLink fieldCode="DE" term="%22Antibiotics%22">Antibiotics</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: • First systematic confirmation of the early cardiovascular developmental toxicity of paromomycin: For the first time, using zebrafish embryos as a model, it was clarified that paromomycin, at environmentally relevant exposure concentrations, can induce cardiovascular developmental damage in early embryos in a dose- and time-dependent manner. • Innovatively integrating network toxicology, molecular docking, and transcriptomics, this study overcomes the limitations of single-method approaches and establishes a comprehensive mechanistic framework from toxic phenotypes to targets and pathways. • It not only clarifies the molecular mechanism of paromomycin-induced cardiovascular toxicity, but also provides direct experimental evidence for the safe use and ecological risk assessment of paromomycin based on the toxicity results at environmentally relevant concentrations, thereby bridging the gap between basic research and practical application. Paromomycin, a broad-spectrum aminoglycoside antibiotic, is widely used in both clinical and agricultural fields. However, its environmental impact and potential developmental toxicity have raised growing concerns. This study systematically evaluates the adverse effects of paromomycin on early cardiovascular development in zebrafish embryos from multiple biological perspectives. Zebrafish embryos were exposed to environmental concentrations of paromomycin (0, 0.3, 3, 30, and 300 μg/mL) for 5 days. Comprehensive assessments were then performed through morphological observations, cardiovascular phenotype analysis, behavioral evaluations, and integrated analysis. The results revealed that exposure to paromomycin induced dose dependent developmental damage, including decreased survival rates, delayed hatching, and increased malformation rates. Detailed cardiovascular assessments confirmed significant abnormalities in both cardiac function and vascular development. Molecular level investigations further elucidated the underlying mechanisms, showing that paromomycin exposure elevated reactive oxygen species levels, triggering oxidative stress, inducing apoptosis in the pericardial region, and disrupting the expression of key cardiovascular developmental genes. Integrating network toxicology, molecular docking, and transcriptomic analyses, this study identified six key signaling pathways involved in paromomycin induced cardiovascular toxicity. These findings provide a comprehensive mechanistic framework for understanding the developmental cardiovascular toxicity of paromomycin at environmentally relevant concentrations and offer valuable insights for the safe application and risk management of this widely used antibiotic. [Display omitted] [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Aquatic Toxicology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.aquatox.2026.107795 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Cardiovascular development Type: general – SubjectFull: Developmental toxicology Type: general – SubjectFull: Transcriptomes Type: general – SubjectFull: Toxicology Type: general – SubjectFull: Oxidative stress Type: general – SubjectFull: Brachydanio Type: general – SubjectFull: Molecular docking Type: general – SubjectFull: Antibiotics Type: general Titles: – TitleFull: The adverse effects of paromomycin on early cardiovascular development in zebrafish (Danio rerio). Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lin, Yuan – PersonEntity: Name: NameFull: Lei, Yuqing – PersonEntity: Name: NameFull: Zhong, Yijing – PersonEntity: Name: NameFull: Cai, Mingfeng – PersonEntity: Name: NameFull: Wang, Zhenqi – PersonEntity: Name: NameFull: Xie, Haohang – PersonEntity: Name: NameFull: Cao, Hua – PersonEntity: Name: NameFull: Wang, Xinrui IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 0166445X Numbering: – Type: volume Value: 295 Titles: – TitleFull: Aquatic Toxicology Type: main |
| ResultId | 1 |
