Fetal Cardiac Extracellular Matrix Incorporated Into Silk Scaffolds Leads to Beneficial Cardiovascular Patch Remodeling in a Large Animal Model of RVOT Surgical Repair.
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| Title: | Fetal Cardiac Extracellular Matrix Incorporated Into Silk Scaffolds Leads to Beneficial Cardiovascular Patch Remodeling in a Large Animal Model of RVOT Surgical Repair. |
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| Authors: | Porter, Elizabeth C.1 (AUTHOR), Lai, Yan‐Ru2 (AUTHOR), Stoppel, Whitney L.2 (AUTHOR), Perreault, Luke R.2 (AUTHOR), Kaplan, David L.2 (AUTHOR), Kudej, Raymond K.3 (AUTHOR), Black, Lauren D.1,2 (AUTHOR) lauren.black@tufts.edu |
| Source: | Journal of Biomedical Materials Research, Part B: Applied Biomaterials. Apr2026, Vol. 114 Issue 4, p1-17. 17p. |
| Subjects: | Extracellular matrix, Silk fibroin, Fibrosis, Ventricular outflow obstruction, Vascular remodeling, Tetralogy of Fallot, Neovascularization, Cardiac regeneration |
| Abstract: | Approximately 50% of patients that undergo surgical reconstruction to repair the congenital heart defect tetralogy of Fallot (ToF) experience adverse effects by the age of 50. These effects can be linked to the poor healing response elicited by the synthetic materials in the commonly used cardiovascular patches. Extracellular matrix (ECM) biomaterial‐based therapies, on the other hand, have been shown to elicit positive healing responses in various tissues and pathologies, including those in the heart. Few studies, however, have been directed towards the development of an ECM‐based cardiovascular patch that can realistically fill the voids in tissue present in ToF patients. Furthermore, cardiac ECM is typically derived from adult sources, despite knowledge that only fetal and neonatal rodents can fully regenerate functional cardiac tissue. Here, we demonstrate the widening of the RVOT in young porcine with a novel cardiovascular patch fabricated from fetal cardiac ECM and silk fibroin (cECM‐silk). Histological analyses show our fetal cECM‐silk patches improve patch‐tissue integration and patch degradation compared to a clinical‐standard GORE ACUSEAL cardiovascular patch, as a representative of expanded polytetrafluoroethylene (ePTFE)‐based material. Our fetal cardiac ECM‐silk patches also improve vascularization in and around the patch and reduce the detrimental fibrotic response typically seen with the GORE ACUSEAL patches. Finally, the culture of activated cardiac fibroblasts in vitro showed analogous reduction in fibroblast activation when treated with solubilized fetal cECM. Our results suggest that fetal cECM is a potential alternative cardiovascular patch material that enables improved healing responses over synthetic materials post‐surgical repair of ToF and other CHDs. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biomedical Materials Research, Part B: Applied Biomaterials is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 193321241 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Fetal Cardiac Extracellular Matrix Incorporated Into Silk Scaffolds Leads to Beneficial Cardiovascular Patch Remodeling in a Large Animal Model of RVOT Surgical Repair. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Porter%2C+Elizabeth+C%2E%22">Porter, Elizabeth C.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lai%2C+Yan‐Ru%22">Lai, Yan‐Ru</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stoppel%2C+Whitney+L%2E%22">Stoppel, Whitney L.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Perreault%2C+Luke+R%2E%22">Perreault, Luke R.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kaplan%2C+David+L%2E%22">Kaplan, David L.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kudej%2C+Raymond+K%2E%22">Kudej, Raymond K.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Black%2C+Lauren+D%2E%22">Black, Lauren D.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> lauren.black@tufts.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Materials+Research%2C+Part+B%3A+Applied+Biomaterials%22">Journal of Biomedical Materials Research, Part B: Applied Biomaterials</searchLink>. Apr2026, Vol. 114 Issue 4, p1-17. 17p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Extracellular+matrix%22">Extracellular matrix</searchLink><br /><searchLink fieldCode="DE" term="%22Silk+fibroin%22">Silk fibroin</searchLink><br /><searchLink fieldCode="DE" term="%22Fibrosis%22">Fibrosis</searchLink><br /><searchLink fieldCode="DE" term="%22Ventricular+outflow+obstruction%22">Ventricular outflow obstruction</searchLink><br /><searchLink fieldCode="DE" term="%22Vascular+remodeling%22">Vascular remodeling</searchLink><br /><searchLink fieldCode="DE" term="%22Tetralogy+of+Fallot%22">Tetralogy of Fallot</searchLink><br /><searchLink fieldCode="DE" term="%22Neovascularization%22">Neovascularization</searchLink><br /><searchLink fieldCode="DE" term="%22Cardiac+regeneration%22">Cardiac regeneration</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Approximately 50% of patients that undergo surgical reconstruction to repair the congenital heart defect tetralogy of Fallot (ToF) experience adverse effects by the age of 50. These effects can be linked to the poor healing response elicited by the synthetic materials in the commonly used cardiovascular patches. Extracellular matrix (ECM) biomaterial‐based therapies, on the other hand, have been shown to elicit positive healing responses in various tissues and pathologies, including those in the heart. Few studies, however, have been directed towards the development of an ECM‐based cardiovascular patch that can realistically fill the voids in tissue present in ToF patients. Furthermore, cardiac ECM is typically derived from adult sources, despite knowledge that only fetal and neonatal rodents can fully regenerate functional cardiac tissue. Here, we demonstrate the widening of the RVOT in young porcine with a novel cardiovascular patch fabricated from fetal cardiac ECM and silk fibroin (cECM‐silk). Histological analyses show our fetal cECM‐silk patches improve patch‐tissue integration and patch degradation compared to a clinical‐standard GORE ACUSEAL cardiovascular patch, as a representative of expanded polytetrafluoroethylene (ePTFE)‐based material. Our fetal cardiac ECM‐silk patches also improve vascularization in and around the patch and reduce the detrimental fibrotic response typically seen with the GORE ACUSEAL patches. Finally, the culture of activated cardiac fibroblasts in vitro showed analogous reduction in fibroblast activation when treated with solubilized fetal cECM. Our results suggest that fetal cECM is a potential alternative cardiovascular patch material that enables improved healing responses over synthetic materials post‐surgical repair of ToF and other CHDs. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biomedical Materials Research, Part B: Applied Biomaterials is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/jbm.b.70065 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 17 StartPage: 1 Subjects: – SubjectFull: Extracellular matrix Type: general – SubjectFull: Silk fibroin Type: general – SubjectFull: Fibrosis Type: general – SubjectFull: Ventricular outflow obstruction Type: general – SubjectFull: Vascular remodeling Type: general – SubjectFull: Tetralogy of Fallot Type: general – SubjectFull: Neovascularization Type: general – SubjectFull: Cardiac regeneration Type: general Titles: – TitleFull: Fetal Cardiac Extracellular Matrix Incorporated Into Silk Scaffolds Leads to Beneficial Cardiovascular Patch Remodeling in a Large Animal Model of RVOT Surgical Repair. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Porter, Elizabeth C. – PersonEntity: Name: NameFull: Lai, Yan‐Ru – PersonEntity: Name: NameFull: Stoppel, Whitney L. – PersonEntity: Name: NameFull: Perreault, Luke R. – PersonEntity: Name: NameFull: Kaplan, David L. – PersonEntity: Name: NameFull: Kudej, Raymond K. – PersonEntity: Name: NameFull: Black, Lauren D. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 15524973 Numbering: – Type: volume Value: 114 – Type: issue Value: 4 Titles: – TitleFull: Journal of Biomedical Materials Research, Part B: Applied Biomaterials Type: main |
| ResultId | 1 |
