Nano-dicalcium silicate promotes angiogenesis via enhancing endothelial cell proliferation, migration and tube formation.
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| Title: | Nano-dicalcium silicate promotes angiogenesis via enhancing endothelial cell proliferation, migration and tube formation. |
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| Authors: | Wang, Ruolan1 (AUTHOR), Lin, Yuntao1,2,3,4 (AUTHOR), Chen, Yuling1,2,3,4 (AUTHOR), Yang, Hongyu1,2,3,4 (AUTHOR) yanghongyu@pkuszh.com |
| Source: | Journal of Materials Science: Materials in Medicine. 4/2/2026, Vol. 37 Issue 1, p1-15. 15p. |
| Subjects: | Endothelial cells, Calcium silicates, Bone regeneration, Cellular signal transduction, Bone growth, Neovascularization, Cell motility |
| Abstract: | Bone defects, especially critical-sized ones, pose a significant challenge in clinical orthopedics due to their impaired self-regeneration ability. Our previous experiments have confirmed that dicalcium silicate nanoparticles(nC2S) exhibit excellent osteogenic activity, but whether they also possesses good angiogenic activity remains unelucidated. In this study, we established a co-culture model of nC2S and HUVECs to further explore its angiogenic effect. CCK-8 assay, immunofluorescence (IF) staining, and Western Blotting (WB) analysis were utilized to systematically assess the effects of nC₂S on the proliferative capacity, migratory potential, and tube formation ability of HUVECs—three core functional hallmarks of angiogenesis. In vivo, nC₂S scaffolds were implanted into bone defect models to validate their ability in promoting neovascularization within bone defect sites. Furthermore, RNA sequencing was employed to explore the potential molecular mechanisms of nC₂S-induced angiogenesis. We found that nC2S can promote HUVECs proliferation, migration, and tube formation. RNA-seq result showed upregulation of MAPK, PI3K/Akt pathway. Thus, we speculate that nC2S may promote angiogenesis through cell proliferation. This study aims to investigate the role of dicalcium silicate in promoting angiogenesis during bone defect repair, and to provide an important theoretical basis and practical guidance for the development of novel bone regeneration materials. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Materials Science: Materials in Medicine is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 193684930 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Nano-dicalcium silicate promotes angiogenesis via enhancing endothelial cell proliferation, migration and tube formation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wang%2C+Ruolan%22">Wang, Ruolan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lin%2C+Yuntao%22">Lin, Yuntao</searchLink><relatesTo>1,2,3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Yuling%22">Chen, Yuling</searchLink><relatesTo>1,2,3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Hongyu%22">Yang, Hongyu</searchLink><relatesTo>1,2,3,4</relatesTo> (AUTHOR)<i> yanghongyu@pkuszh.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Materials+Science%3A+Materials+in+Medicine%22">Journal of Materials Science: Materials in Medicine</searchLink>. 4/2/2026, Vol. 37 Issue 1, p1-15. 15p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Endothelial+cells%22">Endothelial cells</searchLink><br /><searchLink fieldCode="DE" term="%22Calcium+silicates%22">Calcium silicates</searchLink><br /><searchLink fieldCode="DE" term="%22Bone+regeneration%22">Bone regeneration</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Bone+growth%22">Bone growth</searchLink><br /><searchLink fieldCode="DE" term="%22Neovascularization%22">Neovascularization</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+motility%22">Cell motility</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Bone defects, especially critical-sized ones, pose a significant challenge in clinical orthopedics due to their impaired self-regeneration ability. Our previous experiments have confirmed that dicalcium silicate nanoparticles(nC2S) exhibit excellent osteogenic activity, but whether they also possesses good angiogenic activity remains unelucidated. In this study, we established a co-culture model of nC2S and HUVECs to further explore its angiogenic effect. CCK-8 assay, immunofluorescence (IF) staining, and Western Blotting (WB) analysis were utilized to systematically assess the effects of nC₂S on the proliferative capacity, migratory potential, and tube formation ability of HUVECs—three core functional hallmarks of angiogenesis. In vivo, nC₂S scaffolds were implanted into bone defect models to validate their ability in promoting neovascularization within bone defect sites. Furthermore, RNA sequencing was employed to explore the potential molecular mechanisms of nC₂S-induced angiogenesis. We found that nC2S can promote HUVECs proliferation, migration, and tube formation. RNA-seq result showed upregulation of MAPK, PI3K/Akt pathway. Thus, we speculate that nC2S may promote angiogenesis through cell proliferation. This study aims to investigate the role of dicalcium silicate in promoting angiogenesis during bone defect repair, and to provide an important theoretical basis and practical guidance for the development of novel bone regeneration materials. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Materials Science: Materials in Medicine is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10856-026-07033-2 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 1 Subjects: – SubjectFull: Endothelial cells Type: general – SubjectFull: Calcium silicates Type: general – SubjectFull: Bone regeneration Type: general – SubjectFull: Cellular signal transduction Type: general – SubjectFull: Bone growth Type: general – SubjectFull: Neovascularization Type: general – SubjectFull: Cell motility Type: general Titles: – TitleFull: Nano-dicalcium silicate promotes angiogenesis via enhancing endothelial cell proliferation, migration and tube formation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wang, Ruolan – PersonEntity: Name: NameFull: Lin, Yuntao – PersonEntity: Name: NameFull: Chen, Yuling – PersonEntity: Name: NameFull: Yang, Hongyu IsPartOfRelationships: – BibEntity: Dates: – D: 02 M: 04 Text: 4/2/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 09574530 Numbering: – Type: volume Value: 37 – Type: issue Value: 1 Titles: – TitleFull: Journal of Materials Science: Materials in Medicine Type: main |
| ResultId | 1 |
