View in EDS

ARF6 integrates auxin and gibberellin signaling to promote stone cell lignification in pear via the HB49‐MYB169 module.

Saved in:
Bibliographic Details
Title: ARF6 integrates auxin and gibberellin signaling to promote stone cell lignification in pear via the HB49‐MYB169 module.
Authors: Shan, Yanfei1 (AUTHOR), Chen, Shulin2 (AUTHOR), Cai, Jianfa1 (AUTHOR), Zhou, Zheng2 (AUTHOR), Pan, Chong2 (AUTHOR), Liu, Jiahao2 (AUTHOR), Zheng, Pengfei2 (AUTHOR), Li, Cheng1 (AUTHOR), Liu, Yueyuan3 (AUTHOR), Xue, Cheng2 (AUTHOR) xcheng@sdau.edu.cn, Wu, Jun1,3 (AUTHOR) wujun@njau.edu.cn
Source: New Phytologist. Jun2026, Vol. 250 Issue 6, p3747-3768. 22p.
Subjects: Auxin, Lignification, Plant hormones, Fruit quality, Transcription factors, Genetic transcription regulation
Abstract: Summary: Stone cells originate from secondary cell wall thickening and contain abundant lignin. Their excessive accumulation compromises pear fruit quality, yet the endogenous hormonal mechanisms governing stone cell formation remain unclear.Here, co‐expression network analysis using transcriptome data – the flesh of 206 sand pear accessions sampled at the critical stage of stone cell formation and multitissues of 'Dangshansuli' – showed that auxin biosynthetic gene YUCCA6 and auxin signaling activator ARF6 were strongly co‐expressed with lignin‐related genes.A positive correlation was observed between endogenous indole‐3‐butyric acid (IBA) levels and the rate of stone cell accumulation. Moreover, both YUCCA6 overexpression and exogenous IBA treatment enhanced lignin deposition in pear, demonstrating that auxin promoted stone cell lignification. Additionally, ARF6 promoted stone cell lignification. Mechanistic analyses revealed that ARF6 activated HB49, which subsequently upregulated MYB169, a key transcriptional activator of lignin biosynthesis, thereby promoting stone cell lignification. Notably, ARF6 interacted with repressors IAA27 or RGL3, which suppressed HB49 activation; this repression was alleviated by auxin or gibberellin treatment, which restored ARF6‐mediated activation of HB49.These results establish ARF6 as an integrator of auxin and gibberellin signaling pathways and provide new mechanistic insights into auxin–gibberellin crosstalk in regulating stone cell lignification. [ABSTRACT FROM AUTHOR]
Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 193890629
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: ARF6 integrates auxin and gibberellin signaling to promote stone cell lignification in pear via the HB49‐MYB169 module.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Shan%2C+Yanfei%22">Shan, Yanfei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Shulin%22">Chen, Shulin</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cai%2C+Jianfa%22">Cai, Jianfa</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhou%2C+Zheng%22">Zhou, Zheng</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pan%2C+Chong%22">Pan, Chong</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Jiahao%22">Liu, Jiahao</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zheng%2C+Pengfei%22">Zheng, Pengfei</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Cheng%22">Li, Cheng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Yueyuan%22">Liu, Yueyuan</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xue%2C+Cheng%22">Xue, Cheng</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> xcheng@sdau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Wu%2C+Jun%22">Wu, Jun</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> wujun@njau.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22New+Phytologist%22">New Phytologist</searchLink>. Jun2026, Vol. 250 Issue 6, p3747-3768. 22p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Auxin%22">Auxin</searchLink><br /><searchLink fieldCode="DE" term="%22Lignification%22">Lignification</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+hormones%22">Plant hormones</searchLink><br /><searchLink fieldCode="DE" term="%22Fruit+quality%22">Fruit quality</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+transcription+regulation%22">Genetic transcription regulation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Summary: Stone cells originate from secondary cell wall thickening and contain abundant lignin. Their excessive accumulation compromises pear fruit quality, yet the endogenous hormonal mechanisms governing stone cell formation remain unclear.Here, co‐expression network analysis using transcriptome data – the flesh of 206 sand pear accessions sampled at the critical stage of stone cell formation and multitissues of 'Dangshansuli' – showed that auxin biosynthetic gene YUCCA6 and auxin signaling activator ARF6 were strongly co‐expressed with lignin‐related genes.A positive correlation was observed between endogenous indole‐3‐butyric acid (IBA) levels and the rate of stone cell accumulation. Moreover, both YUCCA6 overexpression and exogenous IBA treatment enhanced lignin deposition in pear, demonstrating that auxin promoted stone cell lignification. Additionally, ARF6 promoted stone cell lignification. Mechanistic analyses revealed that ARF6 activated HB49, which subsequently upregulated MYB169, a key transcriptional activator of lignin biosynthesis, thereby promoting stone cell lignification. Notably, ARF6 interacted with repressors IAA27 or RGL3, which suppressed HB49 activation; this repression was alleviated by auxin or gibberellin treatment, which restored ARF6‐mediated activation of HB49.These results establish ARF6 as an integrator of auxin and gibberellin signaling pathways and provide new mechanistic insights into auxin–gibberellin crosstalk in regulating stone cell lignification. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=193890629
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/nph.71141
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 22
        StartPage: 3747
    Subjects:
      – SubjectFull: Auxin
        Type: general
      – SubjectFull: Lignification
        Type: general
      – SubjectFull: Plant hormones
        Type: general
      – SubjectFull: Fruit quality
        Type: general
      – SubjectFull: Transcription factors
        Type: general
      – SubjectFull: Genetic transcription regulation
        Type: general
    Titles:
      – TitleFull: ARF6 integrates auxin and gibberellin signaling to promote stone cell lignification in pear via the HB49‐MYB169 module.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Shan, Yanfei
      – PersonEntity:
          Name:
            NameFull: Chen, Shulin
      – PersonEntity:
          Name:
            NameFull: Cai, Jianfa
      – PersonEntity:
          Name:
            NameFull: Zhou, Zheng
      – PersonEntity:
          Name:
            NameFull: Pan, Chong
      – PersonEntity:
          Name:
            NameFull: Liu, Jiahao
      – PersonEntity:
          Name:
            NameFull: Zheng, Pengfei
      – PersonEntity:
          Name:
            NameFull: Li, Cheng
      – PersonEntity:
          Name:
            NameFull: Liu, Yueyuan
      – PersonEntity:
          Name:
            NameFull: Xue, Cheng
      – PersonEntity:
          Name:
            NameFull: Wu, Jun
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 15
              M: 06
              Text: Jun2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 0028646X
          Numbering:
            – Type: volume
              Value: 250
            – Type: issue
              Value: 6
          Titles:
            – TitleFull: New Phytologist
              Type: main
ResultId 1