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Cyclophilin A‐mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.

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Title: Cyclophilin A‐mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.
Authors: Goto, Takashi1,2 (AUTHOR) tgoto@mbg.au.dk, Andersen, Kasper Røjkjær1 (AUTHOR), Bamba, Masaru3,4 (AUTHOR), Sato, Shusei3 (AUTHOR), Sugawara, Masayuki5 (AUTHOR), Minamisawa, Kiwamu3 (AUTHOR), Kawaguchi, Masayoshi2,6 (AUTHOR), Stougaard, Jens1 (AUTHOR), Kawaharada, Yasuyuki7 (AUTHOR) yasuyuki@iwate-u.ac.jp
Source: New Phytologist. Jun2026, Vol. 250 Issue 6, p3932-3945. 14p.
Subjects: Cyclophilins, Isomerization, Immunoregulation, Symbiosis, Lotus japonicus, Legumes
Abstract: Summary: In most legume‐rhizobium symbioses, rhizobial colonization occurs through host‐derived intracellular infection threads, which enable rhizobial recruitment while presumably modulating the host immune system to prevent rejection. To investigate post‐translational regulation of immune responses during rhizobial infection, we focused on Cyclophilin A (CyPA), a peptidyl‐prolyl cis/trans isomerase.The model legume Lotus japonicus encodes three canonical CyPA genes. Using CRISPR/Cas9 mutagenesis, structural modeling, and phylogenomics, we characterized LjCyPA1 as essential for normal intracellular infection by compatible rhizobia. A gain‐of‐function LjCyPA1 variant in a soybean cultivar promoted symbiosis with both compatible and incompatible rhizobia.Functional association between LjCyPA1 and the immune hub protein LjRIN4 is essential for symbiosis. The putative cis‐LjRIN4 promoted intracellular rhizobial infection, while the putative trans‐LjRIN4 suppressed it. LjCyPA1 and LjRIN4 acted in concert with the rhizobial type III secretion system (T3SS), highlighting a cooperative role between host and symbiont in facilitating infection.Our results contribute to the understanding of how legumes accept symbiotic partners while balancing immune responses. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
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Abstract:Summary: In most legume‐rhizobium symbioses, rhizobial colonization occurs through host‐derived intracellular infection threads, which enable rhizobial recruitment while presumably modulating the host immune system to prevent rejection. To investigate post‐translational regulation of immune responses during rhizobial infection, we focused on Cyclophilin A (CyPA), a peptidyl‐prolyl cis/trans isomerase.The model legume Lotus japonicus encodes three canonical CyPA genes. Using CRISPR/Cas9 mutagenesis, structural modeling, and phylogenomics, we characterized LjCyPA1 as essential for normal intracellular infection by compatible rhizobia. A gain‐of‐function LjCyPA1 variant in a soybean cultivar promoted symbiosis with both compatible and incompatible rhizobia.Functional association between LjCyPA1 and the immune hub protein LjRIN4 is essential for symbiosis. The putative cis‐LjRIN4 promoted intracellular rhizobial infection, while the putative trans‐LjRIN4 suppressed it. LjCyPA1 and LjRIN4 acted in concert with the rhizobial type III secretion system (T3SS), highlighting a cooperative role between host and symbiont in facilitating infection.Our results contribute to the understanding of how legumes accept symbiotic partners while balancing immune responses. [ABSTRACT FROM AUTHOR]
ISSN:0028646X
DOI:10.1111/nph.71147