View in EDS

Cyclophilin A‐mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.

Saved in:
Bibliographic Details
Title: Cyclophilin A‐mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.
Authors: Goto, Takashi1,2 (AUTHOR) tgoto@mbg.au.dk, Andersen, Kasper Røjkjær1 (AUTHOR), Bamba, Masaru3,4 (AUTHOR), Sato, Shusei3 (AUTHOR), Sugawara, Masayuki5 (AUTHOR), Minamisawa, Kiwamu3 (AUTHOR), Kawaguchi, Masayoshi2,6 (AUTHOR), Stougaard, Jens1 (AUTHOR), Kawaharada, Yasuyuki7 (AUTHOR) yasuyuki@iwate-u.ac.jp
Source: New Phytologist. Jun2026, Vol. 250 Issue 6, p3932-3945. 14p.
Subjects: Cyclophilins, Isomerization, Immunoregulation, Symbiosis, Lotus japonicus, Legumes
Abstract: Summary: In most legume‐rhizobium symbioses, rhizobial colonization occurs through host‐derived intracellular infection threads, which enable rhizobial recruitment while presumably modulating the host immune system to prevent rejection. To investigate post‐translational regulation of immune responses during rhizobial infection, we focused on Cyclophilin A (CyPA), a peptidyl‐prolyl cis/trans isomerase.The model legume Lotus japonicus encodes three canonical CyPA genes. Using CRISPR/Cas9 mutagenesis, structural modeling, and phylogenomics, we characterized LjCyPA1 as essential for normal intracellular infection by compatible rhizobia. A gain‐of‐function LjCyPA1 variant in a soybean cultivar promoted symbiosis with both compatible and incompatible rhizobia.Functional association between LjCyPA1 and the immune hub protein LjRIN4 is essential for symbiosis. The putative cis‐LjRIN4 promoted intracellular rhizobial infection, while the putative trans‐LjRIN4 suppressed it. LjCyPA1 and LjRIN4 acted in concert with the rhizobial type III secretion system (T3SS), highlighting a cooperative role between host and symbiont in facilitating infection.Our results contribute to the understanding of how legumes accept symbiotic partners while balancing immune responses. [ABSTRACT FROM AUTHOR]
Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 193890630
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Cyclophilin A‐mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Goto%2C+Takashi%22">Goto, Takashi</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> tgoto@mbg.au.dk</i><br /><searchLink fieldCode="AR" term="%22Andersen%2C+Kasper+Røjkjær%22">Andersen, Kasper Røjkjær</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bamba%2C+Masaru%22">Bamba, Masaru</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sato%2C+Shusei%22">Sato, Shusei</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sugawara%2C+Masayuki%22">Sugawara, Masayuki</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Minamisawa%2C+Kiwamu%22">Minamisawa, Kiwamu</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kawaguchi%2C+Masayoshi%22">Kawaguchi, Masayoshi</searchLink><relatesTo>2,6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stougaard%2C+Jens%22">Stougaard, Jens</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kawaharada%2C+Yasuyuki%22">Kawaharada, Yasuyuki</searchLink><relatesTo>7</relatesTo> (AUTHOR)<i> yasuyuki@iwate-u.ac.jp</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22New+Phytologist%22">New Phytologist</searchLink>. Jun2026, Vol. 250 Issue 6, p3932-3945. 14p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Cyclophilins%22">Cyclophilins</searchLink><br /><searchLink fieldCode="DE" term="%22Isomerization%22">Isomerization</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoregulation%22">Immunoregulation</searchLink><br /><searchLink fieldCode="DE" term="%22Symbiosis%22">Symbiosis</searchLink><br /><searchLink fieldCode="DE" term="%22Lotus+japonicus%22">Lotus japonicus</searchLink><br /><searchLink fieldCode="DE" term="%22Legumes%22">Legumes</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Summary: In most legume‐rhizobium symbioses, rhizobial colonization occurs through host‐derived intracellular infection threads, which enable rhizobial recruitment while presumably modulating the host immune system to prevent rejection. To investigate post‐translational regulation of immune responses during rhizobial infection, we focused on Cyclophilin A (CyPA), a peptidyl‐prolyl cis/trans isomerase.The model legume Lotus japonicus encodes three canonical CyPA genes. Using CRISPR/Cas9 mutagenesis, structural modeling, and phylogenomics, we characterized LjCyPA1 as essential for normal intracellular infection by compatible rhizobia. A gain‐of‐function LjCyPA1 variant in a soybean cultivar promoted symbiosis with both compatible and incompatible rhizobia.Functional association between LjCyPA1 and the immune hub protein LjRIN4 is essential for symbiosis. The putative cis‐LjRIN4 promoted intracellular rhizobial infection, while the putative trans‐LjRIN4 suppressed it. LjCyPA1 and LjRIN4 acted in concert with the rhizobial type III secretion system (T3SS), highlighting a cooperative role between host and symbiont in facilitating infection.Our results contribute to the understanding of how legumes accept symbiotic partners while balancing immune responses. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=193890630
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/nph.71147
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 14
        StartPage: 3932
    Subjects:
      – SubjectFull: Cyclophilins
        Type: general
      – SubjectFull: Isomerization
        Type: general
      – SubjectFull: Immunoregulation
        Type: general
      – SubjectFull: Symbiosis
        Type: general
      – SubjectFull: Lotus japonicus
        Type: general
      – SubjectFull: Legumes
        Type: general
    Titles:
      – TitleFull: Cyclophilin A‐mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Goto, Takashi
      – PersonEntity:
          Name:
            NameFull: Andersen, Kasper Røjkjær
      – PersonEntity:
          Name:
            NameFull: Bamba, Masaru
      – PersonEntity:
          Name:
            NameFull: Sato, Shusei
      – PersonEntity:
          Name:
            NameFull: Sugawara, Masayuki
      – PersonEntity:
          Name:
            NameFull: Minamisawa, Kiwamu
      – PersonEntity:
          Name:
            NameFull: Kawaguchi, Masayoshi
      – PersonEntity:
          Name:
            NameFull: Stougaard, Jens
      – PersonEntity:
          Name:
            NameFull: Kawaharada, Yasuyuki
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 15
              M: 06
              Text: Jun2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 0028646X
          Numbering:
            – Type: volume
              Value: 250
            – Type: issue
              Value: 6
          Titles:
            – TitleFull: New Phytologist
              Type: main
ResultId 1