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Transmembrane ROOM proteins ensure rooms for germ cells by maintaining intercellular bridges.

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Title: Transmembrane ROOM proteins ensure rooms for germ cells by maintaining intercellular bridges.
Authors: Sugiura, Kenta1, Kawasaki, Ichiro1, Kosako, Hidetaka2, Sato, Ken1 sato-ken@gunma-u.ac.jp
Source: Proceedings of the National Academy of Sciences of the United States of America. 5/12/2026, Vol. 123 Issue 19, p1-11. 11p.
Subjects: Germ cells, Cell junctions, Cell compartmentation, Caenorhabditis elegans, Membrane proteins
Abstract: Germ cells of diverse species form a syncytium, which is a multinucleated cell complex, and share cytoplasmic components via intercellular bridges. Here, we demonstrate that the paralogous transmembrane proteins ROOM-1 and ROOM-2, identified through the proteomic analysis of maternal membrane proteins, are essential for the maintenance of germ cell compartments from the rachis of Caenorhabditis elegans gonads. Although animals lacking each protein are fertile, the loss of both proteins resulted in complete sterility due to compartmentalization failure of individual germ cells in the late larval and adult gonads. Additionally, the ROOM proteins colocalize specifically to the intercellular bridge with F-actin and ANI-2, a protein organizing the intercellular bridge of germ cells. The localizations of ANI-2 and ROOM-1/2 on the rachis bridge were interdependent. Thus, the transmembrane ROOM proteins redundantly function to ensure "rooms" for germ cells by maintaining the intercellular bridges. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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PubType: Academic Journal
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  Data: Transmembrane ROOM proteins ensure rooms for germ cells by maintaining intercellular bridges.
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  Data: <searchLink fieldCode="AR" term="%22Sugiura%2C+Kenta%22">Sugiura, Kenta</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kawasaki%2C+Ichiro%22">Kawasaki, Ichiro</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kosako%2C+Hidetaka%22">Kosako, Hidetaka</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sato%2C+Ken%22">Sato, Ken</searchLink><relatesTo>1</relatesTo><i> sato-ken@gunma-u.ac.jp</i>
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  Data: <searchLink fieldCode="DE" term="%22Germ+cells%22">Germ cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+junctions%22">Cell junctions</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+compartmentation%22">Cell compartmentation</searchLink><br /><searchLink fieldCode="DE" term="%22Caenorhabditis+elegans%22">Caenorhabditis elegans</searchLink><br /><searchLink fieldCode="DE" term="%22Membrane+proteins%22">Membrane proteins</searchLink>
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  Label: Abstract
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  Data: Germ cells of diverse species form a syncytium, which is a multinucleated cell complex, and share cytoplasmic components via intercellular bridges. Here, we demonstrate that the paralogous transmembrane proteins ROOM-1 and ROOM-2, identified through the proteomic analysis of maternal membrane proteins, are essential for the maintenance of germ cell compartments from the rachis of Caenorhabditis elegans gonads. Although animals lacking each protein are fertile, the loss of both proteins resulted in complete sterility due to compartmentalization failure of individual germ cells in the late larval and adult gonads. Additionally, the ROOM proteins colocalize specifically to the intercellular bridge with F-actin and ANI-2, a protein organizing the intercellular bridge of germ cells. The localizations of ANI-2 and ROOM-1/2 on the rachis bridge were interdependent. Thus, the transmembrane ROOM proteins redundantly function to ensure "rooms" for germ cells by maintaining the intercellular bridges. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1073/pnas.2522264123
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      – Code: eng
        Text: English
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        PageCount: 11
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      – SubjectFull: Germ cells
        Type: general
      – SubjectFull: Cell junctions
        Type: general
      – SubjectFull: Cell compartmentation
        Type: general
      – SubjectFull: Caenorhabditis elegans
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      – SubjectFull: Membrane proteins
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      – TitleFull: Transmembrane ROOM proteins ensure rooms for germ cells by maintaining intercellular bridges.
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              M: 05
              Text: 5/12/2026
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              Y: 2026
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