A simple and efficient biodegradation route for nitroaromatic compounds using a nitroreductase from Bacillus subtilis and its "cell-like" immobilized form.
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| Title: | A simple and efficient biodegradation route for nitroaromatic compounds using a nitroreductase from Bacillus subtilis and its "cell-like" immobilized form. |
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| Authors: | Li, Ze1 (AUTHOR), Yang, Ziyi1 (AUTHOR), Zheng, Liangyu1 (AUTHOR) lyzheng@jlu.edu.cn |
| Source: | Journal of Biotechnology. Aug2026, Vol. 416, p94-105. 12p. |
| Subjects: | Nitroaromatic compounds, Immobilized enzymes, Sustainable chemistry, Enzymes, Bacillus subtilis, Biodegradation, Biocatalysis |
| Abstract: | Nitroaromatic compounds are persistent environmental pollutants that pose significant ecological and toxicological risks. Enzymatic reduction of nitroaromatics to the corresponding aromatic amines offers an efficient and selective degradation pathway, whereas simultaneously yielding valuable intermediates for pharmaceutical and agrochemical synthesis. Here, a nitroreductase (NR Bs) from Bacillus subtilis was identified that catalyzed nitroaromatic reduction with exceptional efficiency, achieving a yield greater than 99.0% within 2 h without requiring any metal co-catalyst such as V 2 O 5. To enhance enzyme stability and eliminate repeated cofactor addition, a "cell-like" co-encapsulation system comprising NR Bs , glucose dehydrogenase (GDH), and the cofactor NADH was constructed using ZIF-67 as the encapsulation material. In this design, NR Bs was purified and immobilized on polyvinylpyrrolidone (PVP)-modified ZIF-L (P-ZIF-L), whereas NADH and GDH were co-grafted onto poly(acrylamide-co-diallyldimethylammonium chloride) (PADD) to form a PNG complex. The resulting NR Bs @P-ZIF-L@PNG@ZIF-67 composite exhibited enhanced catalytic activity, stability, and substrate scope. Furthermore, the "cell-like" co-immobilized system retained 65.0 ± 3.5% of its catalytic yield toward aniline formation after seven reaction cycles, whereas the NR Bs –GDH recombinant whole-cell catalyst showed complete deactivation under identical conditions. This work establishes a rapid, simple, green, and sustainable biodegradation strategy for nitroaromatic pollutants, demonstrating the feasibility of using a "cell-like" co-immobilized enzyme system for efficient and reusable biocatalysis. • A rapid, green, and efficient biodegradation route for nitroaromatics is developed. • One-step purification and immobilization of NR Bs is achieved by using P-ZIF-L carrier. • The "cell-like" immobilized system enables sustainable and reusable biodegradation. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 194168053 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A simple and efficient biodegradation route for nitroaromatic compounds using a nitroreductase from Bacillus subtilis and its "cell-like" immobilized form. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Li%2C+Ze%22">Li, Ze</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Ziyi%22">Yang, Ziyi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zheng%2C+Liangyu%22">Zheng, Liangyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lyzheng@jlu.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biotechnology%22">Journal of Biotechnology</searchLink>. Aug2026, Vol. 416, p94-105. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Nitroaromatic+compounds%22">Nitroaromatic compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Immobilized+enzymes%22">Immobilized enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Sustainable+chemistry%22">Sustainable chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Enzymes%22">Enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Bacillus+subtilis%22">Bacillus subtilis</searchLink><br /><searchLink fieldCode="DE" term="%22Biodegradation%22">Biodegradation</searchLink><br /><searchLink fieldCode="DE" term="%22Biocatalysis%22">Biocatalysis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Nitroaromatic compounds are persistent environmental pollutants that pose significant ecological and toxicological risks. Enzymatic reduction of nitroaromatics to the corresponding aromatic amines offers an efficient and selective degradation pathway, whereas simultaneously yielding valuable intermediates for pharmaceutical and agrochemical synthesis. Here, a nitroreductase (NR Bs) from Bacillus subtilis was identified that catalyzed nitroaromatic reduction with exceptional efficiency, achieving a yield greater than 99.0% within 2 h without requiring any metal co-catalyst such as V 2 O 5. To enhance enzyme stability and eliminate repeated cofactor addition, a "cell-like" co-encapsulation system comprising NR Bs , glucose dehydrogenase (GDH), and the cofactor NADH was constructed using ZIF-67 as the encapsulation material. In this design, NR Bs was purified and immobilized on polyvinylpyrrolidone (PVP)-modified ZIF-L (P-ZIF-L), whereas NADH and GDH were co-grafted onto poly(acrylamide-co-diallyldimethylammonium chloride) (PADD) to form a PNG complex. The resulting NR Bs @P-ZIF-L@PNG@ZIF-67 composite exhibited enhanced catalytic activity, stability, and substrate scope. Furthermore, the "cell-like" co-immobilized system retained 65.0 ± 3.5% of its catalytic yield toward aniline formation after seven reaction cycles, whereas the NR Bs –GDH recombinant whole-cell catalyst showed complete deactivation under identical conditions. This work establishes a rapid, simple, green, and sustainable biodegradation strategy for nitroaromatic pollutants, demonstrating the feasibility of using a "cell-like" co-immobilized enzyme system for efficient and reusable biocatalysis. • A rapid, green, and efficient biodegradation route for nitroaromatics is developed. • One-step purification and immobilization of NR Bs is achieved by using P-ZIF-L carrier. • The "cell-like" immobilized system enables sustainable and reusable biodegradation. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jbiotec.2026.04.024 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 94 Subjects: – SubjectFull: Nitroaromatic compounds Type: general – SubjectFull: Immobilized enzymes Type: general – SubjectFull: Sustainable chemistry Type: general – SubjectFull: Enzymes Type: general – SubjectFull: Bacillus subtilis Type: general – SubjectFull: Biodegradation Type: general – SubjectFull: Biocatalysis Type: general Titles: – TitleFull: A simple and efficient biodegradation route for nitroaromatic compounds using a nitroreductase from Bacillus subtilis and its "cell-like" immobilized form. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Li, Ze – PersonEntity: Name: NameFull: Yang, Ziyi – PersonEntity: Name: NameFull: Zheng, Liangyu IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 01681656 Numbering: – Type: volume Value: 416 Titles: – TitleFull: Journal of Biotechnology Type: main |
| ResultId | 1 |
