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Natural Preservation of Yogurt Using Origanum vulgare and Thymus vulgaris Essential Oils: Microbial, Physicochemical, and Sensory Assessment.

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Title: Natural Preservation of Yogurt Using Origanum vulgare and Thymus vulgaris Essential Oils: Microbial, Physicochemical, and Sensory Assessment.
Authors: Shahin, Hoda Helene1 (AUTHOR), Baroudi, Moomen2 (AUTHOR), Dabboussi, Fouad1 (AUTHOR), El-Mir, Reem1 (AUTHOR), Germanos, Julia1 (AUTHOR), Salma, Rayane3 (AUTHOR), El-Omari, Khaled1,3 (AUTHOR) khaledo@cciat.org.lb, Hebishy, Essam (AUTHOR) ehebishy@wiley.com
Source: Journal of Food Processing & Preservation. 6/1/2026, Vol. 2026, p1-14. 14p.
Subjects: Essential oils, Yogurt microbiology, Oregano, Sensory evaluation, Thymes, Food spoilage, Food preservatives, Anti-infective agents
Abstract: The demand for natural preservatives in food systems has increased interest in plant‐derived essential oils (EOs), particularly Origanum vulgare (OEO) and Thymus vulgaris (TEO), due to their antimicrobial properties. This preliminary study evaluated their antifungal activity and their effectiveness in preserving Lebanese yogurt. Both EOs exhibited strong antifungal activity against Aspergillus niger and Kluyveromyces marxianus, with OEO showing higher potency (MIC = 0.00076%)[v/v]ompared with TEO (MIC = 0.80% [v/v]). In yogurt applications, EO‐treated samples demonstrated delayed microbial spoilage compared with controls. In opened samples, Y&M growth appeared on Day 8 in controls but was delayed to Day 22 in EO‐treated samples. In sealed samples, untreated yogurt exceeded acceptable microbial limits by Day 30, whereas EO‐treated samples maintained microbiological stability under refrigerated storage conditions up to Day 150. Titratable acidity increased from 0.80% to 1.50% by Day 8 in controls, whereas EO‐treated samples reached only 1.40% by Day 150, indicating delayed acidification. Sensory evaluation confirmed maintained acceptability throughout the extended storage period. These findings demonstrate that OEO and TEO effectively inhibit fungal growth and delay spoilage in yogurt, supporting their potential application as natural antimicrobial preservatives in dairy systems. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Food Processing & Preservation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Natural Preservation of Yogurt Using Origanum vulgare and Thymus vulgaris Essential Oils: Microbial, Physicochemical, and Sensory Assessment.
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Food+Processing+%26+Preservation%22">Journal of Food Processing & Preservation</searchLink>. 6/1/2026, Vol. 2026, p1-14. 14p.
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  Data: <searchLink fieldCode="DE" term="%22Essential+oils%22">Essential oils</searchLink><br /><searchLink fieldCode="DE" term="%22Yogurt+microbiology%22">Yogurt microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22Oregano%22">Oregano</searchLink><br /><searchLink fieldCode="DE" term="%22Sensory+evaluation%22">Sensory evaluation</searchLink><br /><searchLink fieldCode="DE" term="%22Thymes%22">Thymes</searchLink><br /><searchLink fieldCode="DE" term="%22Food+spoilage%22">Food spoilage</searchLink><br /><searchLink fieldCode="DE" term="%22Food+preservatives%22">Food preservatives</searchLink><br /><searchLink fieldCode="DE" term="%22Anti-infective+agents%22">Anti-infective agents</searchLink>
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  Label: Abstract
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  Data: The demand for natural preservatives in food systems has increased interest in plant‐derived essential oils (EOs), particularly Origanum vulgare (OEO) and Thymus vulgaris (TEO), due to their antimicrobial properties. This preliminary study evaluated their antifungal activity and their effectiveness in preserving Lebanese yogurt. Both EOs exhibited strong antifungal activity against Aspergillus niger and Kluyveromyces marxianus, with OEO showing higher potency (MIC = 0.00076%)[v/v]ompared with TEO (MIC = 0.80% [v/v]). In yogurt applications, EO‐treated samples demonstrated delayed microbial spoilage compared with controls. In opened samples, Y&M growth appeared on Day 8 in controls but was delayed to Day 22 in EO‐treated samples. In sealed samples, untreated yogurt exceeded acceptable microbial limits by Day 30, whereas EO‐treated samples maintained microbiological stability under refrigerated storage conditions up to Day 150. Titratable acidity increased from 0.80% to 1.50% by Day 8 in controls, whereas EO‐treated samples reached only 1.40% by Day 150, indicating delayed acidification. Sensory evaluation confirmed maintained acceptability throughout the extended storage period. These findings demonstrate that OEO and TEO effectively inhibit fungal growth and delay spoilage in yogurt, supporting their potential application as natural antimicrobial preservatives in dairy systems. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Food Processing & Preservation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1155/jfpp/6081584
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      – Code: eng
        Text: English
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        PageCount: 14
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    Subjects:
      – SubjectFull: Essential oils
        Type: general
      – SubjectFull: Yogurt microbiology
        Type: general
      – SubjectFull: Oregano
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      – SubjectFull: Sensory evaluation
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      – SubjectFull: Thymes
        Type: general
      – SubjectFull: Food spoilage
        Type: general
      – SubjectFull: Food preservatives
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      – SubjectFull: Anti-infective agents
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              M: 06
              Text: 6/1/2026
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              Y: 2026
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