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A cold-responsive fimACD chaperone-usher operon tunes motility and biofilm formation in Pseudomonas fragi D12.

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Title: A cold-responsive fimACD chaperone-usher operon tunes motility and biofilm formation in Pseudomonas fragi D12.
Authors: Li, Shaoyu1,2, Zhang, Xia1,2, Li, Murong1,2, Zhou, Meng1,2, Xing, Zhihao1,2, Zhu, Wenbo3, Liu, Yehui1,2, Li, Qiyun1 liqiyun@jlau.edu.cn, Zang, Xueli4 2296415080@qq.com, Zhang, Sitong1,2 18943132269@163.com
Source: Applied & Environmental Microbiology. May2026, Vol. 92 Issue 5, p1-18. 18p.
Subjects: Cold adaptation, Operons, Biofilms, Cell motility, Bacterial adhesion, Pseudomonas, RNA sequencing
Abstract: Low temperature alters bacterial growth and surface-linked behaviors; however, the genetic role of the associated pilus systems in cold adaptation remains unclear. Here, we used the psychrotolerant tundra isolate Pseudomonas fragi D12 as a model to investigate the transcriptional responses and functional divergence of three fimbrial genes, fimA, fimC, and fimD, through a combination of transcriptome analysis and gene knockout/overexpression assays. RNA-seq analysis revealed that extreme cold stress (4°C) triggered a robust induction of the fim cluster and an adjacent regulatory module comprising an Arc-family DNA-binding protein and an EAL-domain phosphodiesterase. qRT-PCR confirmed the RNA-seq trends. Functional assays demonstrated distinct ecological roles; deletion of fimA increased swimming but reduced swarming, whereas overexpression of fimA led to an increase in swarming. fimC overexpression enhanced swimming, whereas fimC deletion decreased swarming. fimD deletion increased swimming and reduced swarming, while fimD overexpression suppressed swarming. Temperature-gradient experiments further showed that across the three temperatures examined (4°C, 15°C, and 30°C), motility and biofilm formation were the highest at 15°C. Transmission electron microscopy associated these behavioral changes with altered fimbrial density and organization, and growth-curve analysis indicated no major defects in planktonic proliferation. In combination, the data point to a fimbri al apparatus that is transcriptionally responsive to cold and may mechanically modulate the coupling of a single polar flagellum to liquid and solid interfaces, while the genomic context of fimACD remains compatible with local modulation of cyclic di-GMP signaling that has yet to be examined directly. [ABSTRACT FROM AUTHOR]
Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 194351801
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  Label: Title
  Group: Ti
  Data: A cold-responsive fimACD chaperone-usher operon tunes motility and biofilm formation in Pseudomonas fragi D12.
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  Data: <searchLink fieldCode="AR" term="%22Li%2C+Shaoyu%22">Li, Shaoyu</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Xia%22">Zhang, Xia</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Murong%22">Li, Murong</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Meng%22">Zhou, Meng</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xing%2C+Zhihao%22">Xing, Zhihao</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhu%2C+Wenbo%22">Zhu, Wenbo</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Yehui%22">Liu, Yehui</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Qiyun%22">Li, Qiyun</searchLink><relatesTo>1</relatesTo><i> liqiyun@jlau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Zang%2C+Xueli%22">Zang, Xueli</searchLink><relatesTo>4</relatesTo><i> 2296415080@qq.com</i><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Sitong%22">Zhang, Sitong</searchLink><relatesTo>1,2</relatesTo><i> 18943132269@163.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Applied+%26+Environmental+Microbiology%22">Applied & Environmental Microbiology</searchLink>. May2026, Vol. 92 Issue 5, p1-18. 18p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Cold+adaptation%22">Cold adaptation</searchLink><br /><searchLink fieldCode="DE" term="%22Operons%22">Operons</searchLink><br /><searchLink fieldCode="DE" term="%22Biofilms%22">Biofilms</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+motility%22">Cell motility</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+adhesion%22">Bacterial adhesion</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonas%22">Pseudomonas</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Low temperature alters bacterial growth and surface-linked behaviors; however, the genetic role of the associated pilus systems in cold adaptation remains unclear. Here, we used the psychrotolerant tundra isolate Pseudomonas fragi D12 as a model to investigate the transcriptional responses and functional divergence of three fimbrial genes, fimA, fimC, and fimD, through a combination of transcriptome analysis and gene knockout/overexpression assays. RNA-seq analysis revealed that extreme cold stress (4°C) triggered a robust induction of the fim cluster and an adjacent regulatory module comprising an Arc-family DNA-binding protein and an EAL-domain phosphodiesterase. qRT-PCR confirmed the RNA-seq trends. Functional assays demonstrated distinct ecological roles; deletion of fimA increased swimming but reduced swarming, whereas overexpression of fimA led to an increase in swarming. fimC overexpression enhanced swimming, whereas fimC deletion decreased swarming. fimD deletion increased swimming and reduced swarming, while fimD overexpression suppressed swarming. Temperature-gradient experiments further showed that across the three temperatures examined (4°C, 15°C, and 30°C), motility and biofilm formation were the highest at 15°C. Transmission electron microscopy associated these behavioral changes with altered fimbrial density and organization, and growth-curve analysis indicated no major defects in planktonic proliferation. In combination, the data point to a fimbri al apparatus that is transcriptionally responsive to cold and may mechanically modulate the coupling of a single polar flagellum to liquid and solid interfaces, while the genomic context of fimACD remains compatible with local modulation of cyclic di-GMP signaling that has yet to be examined directly. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1128/aem.02472-25
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      – Code: eng
        Text: English
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        PageCount: 18
        StartPage: 1
    Subjects:
      – SubjectFull: Cold adaptation
        Type: general
      – SubjectFull: Operons
        Type: general
      – SubjectFull: Biofilms
        Type: general
      – SubjectFull: Cell motility
        Type: general
      – SubjectFull: Bacterial adhesion
        Type: general
      – SubjectFull: Pseudomonas
        Type: general
      – SubjectFull: RNA sequencing
        Type: general
    Titles:
      – TitleFull: A cold-responsive fimACD chaperone-usher operon tunes motility and biofilm formation in Pseudomonas fragi D12.
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            – D: 01
              M: 05
              Text: May2026
              Type: published
              Y: 2026
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