How far can you go? Extrapolating values of catalytic activity from known protein landscapes in natural and directed evolution.
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| Title: | How far can you go? Extrapolating values of catalytic activity from known protein landscapes in natural and directed evolution. |
|---|---|
| Authors: | Kell, Douglas B.1,2,3 (AUTHOR) dbk@liv.ac.uk, Roberts, Ivayla1 (AUTHOR) |
| Source: | Chemical Society Reviews. 6/8/2026, Vol. 55 Issue 11, p6417-6452. 36p. |
| Subjects: | Catalytic activity, Epistasis (Genetics), Protein engineering, Machine learning, Biocatalysis, Statistical models |
| Abstract: | The number of possible variants representing the landscape of a protein sequence of length N residues, made of the standard unmodified proteinogenic amino acids, is 20N; its exhaustive experimental analysis is consequently intractable. Our focus is on the real and perceived shapes of different fitness landscapes. Epistasis refers to a phenomenon by which the 'best' amino acid at a given residue depends on the nature of the amino acid at one or more other residues. Because of epistasis, real protein landscapes display peaks representing local maxima in which weak mutation/strong-selection regimes can cause evolution to become trapped, leading to landscapes that are rugged. Fortunately, although they are necessarily somewhat rugged, such protein landscapes possess regularities that admit their modelling from more limited experimental data, using the methods of statistics and machine learning. We provide a variety of arguments that for typical proteins of length 300–500 residues some 105 or 106 examples, and in favourable cases even fewer, are likely sufficient to allow a reasonable initial modelling (and accurate predictive exploration) of the entire 20N landscape for properties such as kcat. The distribution of fitness effects (DFE) around an existing wild type is usually reasonably fitted statistically by a gamma distribution. However, we also survey modern ideas, especially extreme value theory, that allow extrapolation from the known, with a focus on methods – especially the Generalised Pareto Distribution – that provide means for generating the statistical likelihood of obtaining activities or fitnesses far greater than those observed in existing populations as measured with what are small numbers. These likelihoods typically decrease exponentially, as do the decreases in errors as a function of the size of the network and of the training data as found by deep neural network models as 'universal approximators'. This is entirely consistent with the large differences between the minuscule amount of available sequence-activity data, that are necessarily local in character, reflecting evolutionary contingency, and the overall distribution (20N, where N might usefully be decreased) that would be expected to contain examples that have much better properties than any observed thus far. This consequently requires careful choices of examples drawn from an extensive distribution (using active learning) for predictive modelling. For instance, a widespread view of a trade-off between catalytic activity and thermostability seems to follow directly from inadequate sampling. All of this has significant implications for the understanding, modelling, and optimisation of experiments in directed evolution and the biocatalysts they produce. [ABSTRACT FROM AUTHOR] |
| Copyright of Chemical Society Reviews is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 194364688 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: How far can you go? Extrapolating values of catalytic activity from known protein landscapes in natural and directed evolution. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kell%2C+Douglas+B%2E%22">Kell, Douglas B.</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> dbk@liv.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Roberts%2C+Ivayla%22">Roberts, Ivayla</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Chemical+Society+Reviews%22">Chemical Society Reviews</searchLink>. 6/8/2026, Vol. 55 Issue 11, p6417-6452. 36p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Catalytic+activity%22">Catalytic activity</searchLink><br /><searchLink fieldCode="DE" term="%22Epistasis+%28Genetics%29%22">Epistasis (Genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+engineering%22">Protein engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Machine+learning%22">Machine learning</searchLink><br /><searchLink fieldCode="DE" term="%22Biocatalysis%22">Biocatalysis</searchLink><br /><searchLink fieldCode="DE" term="%22Statistical+models%22">Statistical models</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The number of possible variants representing the landscape of a protein sequence of length N residues, made of the standard unmodified proteinogenic amino acids, is 20N; its exhaustive experimental analysis is consequently intractable. Our focus is on the real and perceived shapes of different fitness landscapes. Epistasis refers to a phenomenon by which the 'best' amino acid at a given residue depends on the nature of the amino acid at one or more other residues. Because of epistasis, real protein landscapes display peaks representing local maxima in which weak mutation/strong-selection regimes can cause evolution to become trapped, leading to landscapes that are rugged. Fortunately, although they are necessarily somewhat rugged, such protein landscapes possess regularities that admit their modelling from more limited experimental data, using the methods of statistics and machine learning. We provide a variety of arguments that for typical proteins of length 300–500 residues some 105 or 106 examples, and in favourable cases even fewer, are likely sufficient to allow a reasonable initial modelling (and accurate predictive exploration) of the entire 20N landscape for properties such as kcat. The distribution of fitness effects (DFE) around an existing wild type is usually reasonably fitted statistically by a gamma distribution. However, we also survey modern ideas, especially extreme value theory, that allow extrapolation from the known, with a focus on methods – especially the Generalised Pareto Distribution – that provide means for generating the statistical likelihood of obtaining activities or fitnesses far greater than those observed in existing populations as measured with what are small numbers. These likelihoods typically decrease exponentially, as do the decreases in errors as a function of the size of the network and of the training data as found by deep neural network models as 'universal approximators'. This is entirely consistent with the large differences between the minuscule amount of available sequence-activity data, that are necessarily local in character, reflecting evolutionary contingency, and the overall distribution (20N, where N might usefully be decreased) that would be expected to contain examples that have much better properties than any observed thus far. This consequently requires careful choices of examples drawn from an extensive distribution (using active learning) for predictive modelling. For instance, a widespread view of a trade-off between catalytic activity and thermostability seems to follow directly from inadequate sampling. All of this has significant implications for the understanding, modelling, and optimisation of experiments in directed evolution and the biocatalysts they produce. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Chemical Society Reviews is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1039/d5cs01387a Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 36 StartPage: 6417 Subjects: – SubjectFull: Catalytic activity Type: general – SubjectFull: Epistasis (Genetics) Type: general – SubjectFull: Protein engineering Type: general – SubjectFull: Machine learning Type: general – SubjectFull: Biocatalysis Type: general – SubjectFull: Statistical models Type: general Titles: – TitleFull: How far can you go? Extrapolating values of catalytic activity from known protein landscapes in natural and directed evolution. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kell, Douglas B. – PersonEntity: Name: NameFull: Roberts, Ivayla IsPartOfRelationships: – BibEntity: Dates: – D: 08 M: 06 Text: 6/8/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 03060012 Numbering: – Type: volume Value: 55 – Type: issue Value: 11 Titles: – TitleFull: Chemical Society Reviews Type: main |
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