View in EDS

Influence of Er:YAG Laser Dentin Conditioning with End-Firing Versus Side-Firing Tips on Human Dental Pulp–Derived Cell Viability.

Saved in:
Bibliographic Details
Title: Influence of Er:YAG Laser Dentin Conditioning with End-Firing Versus Side-Firing Tips on Human Dental Pulp–Derived Cell Viability.
Authors: Bangarimath, Parvati S.1 (AUTHOR) parusb2000@gmail.com, Merwade, Seema1 (AUTHOR), Kumar N, Kiran1 (AUTHOR), Bhavikatti, Tasmiya M.1 (AUTHOR), Kumari, Vandana1 (AUTHOR)
Source: Photobiomodulation, Photomedicine & Laser Surgery. Jul2026, Vol. 44 Issue 7, p437-447. 11p.
Subjects: Dentin, Cytocompatibility, Ethylenediaminetetraacetic acid, Cell survival, Dental pulp, Endodontics, YAG lasers
Abstract: Introduction: Dentin surface conditioning plays a key role in regenerative endodontics by influencing stem cell viability. Although ethylenediaminetetraacetic acid (EDTA) effectively removes the smear layer, residual chelating agents may compromise cytocompatibility. Er:YAG (erbium-doped yttrium aluminum garnet) laser conditioning offers an alternative approach with minimal chemical residue, and laser tip geometry may further influence biological outcomes. This study evaluated the cytocompatibility of Er:YAG laser dentin conditioning using End firing sapphire and side-firing tips by assessing human dental pulp – derived cell viability. Methods: Thirty-six single-rooted human teeth were decoronated and instrumented to ProTaper F4. Specimens were randomly allocated to four groups (n = 9): untreated control, Er:YAG laser with sapphire tip, Er:YAG laser with side-firing tip, and 17% EDTA. Following dentin conditioning, roots were longitudinally split to expose the canal wall, and human dental pulp–derived cells (1 × 104 cells/specimen) were seeded onto the treated surfaces. Cell viability, as an indirect indicator of cytocompatibility, was assessed using the MTT assay at Days 1, 4, and 7. Data were analyzed using one-way analysis of variance with Bonferroni post hoc tests (α = 0.05). Results: At all time points, the side-firing Er:YAG group demonstrated the highest cell viability, followed by the End firing sapphire-tip laser group. EDTA-conditioned dentin showed significantly lower viability, while the control group maintained baseline viability. Differences between groups became more pronounced over time. Conclusions: Er:YAG laser dentin conditioning enhanced human dental pulp–derived cell viability compared with EDTA, with the side-firing tip yielding the most favorable results. Side-firing Er:YAG tips may therefore provide a more cytocompatible dentin surface for regenerative endodontic applications. [ABSTRACT FROM AUTHOR]
Copyright of Photobiomodulation, Photomedicine & Laser Surgery is the property of Mary Ann Liebert, Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 194489391
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Influence of Er:YAG Laser Dentin Conditioning with End-Firing Versus Side-Firing Tips on Human Dental Pulp–Derived Cell Viability.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Bangarimath%2C+Parvati+S%2E%22">Bangarimath, Parvati S.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> parusb2000@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Merwade%2C+Seema%22">Merwade, Seema</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kumar+N%2C+Kiran%22">Kumar N, Kiran</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bhavikatti%2C+Tasmiya+M%2E%22">Bhavikatti, Tasmiya M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kumari%2C+Vandana%22">Kumari, Vandana</searchLink><relatesTo>1</relatesTo> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Photobiomodulation%2C+Photomedicine+%26+Laser+Surgery%22">Photobiomodulation, Photomedicine & Laser Surgery</searchLink>. Jul2026, Vol. 44 Issue 7, p437-447. 11p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Dentin%22">Dentin</searchLink><br /><searchLink fieldCode="DE" term="%22Cytocompatibility%22">Cytocompatibility</searchLink><br /><searchLink fieldCode="DE" term="%22Ethylenediaminetetraacetic+acid%22">Ethylenediaminetetraacetic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+survival%22">Cell survival</searchLink><br /><searchLink fieldCode="DE" term="%22Dental+pulp%22">Dental pulp</searchLink><br /><searchLink fieldCode="DE" term="%22Endodontics%22">Endodontics</searchLink><br /><searchLink fieldCode="DE" term="%22YAG+lasers%22">YAG lasers</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Introduction: Dentin surface conditioning plays a key role in regenerative endodontics by influencing stem cell viability. Although ethylenediaminetetraacetic acid (EDTA) effectively removes the smear layer, residual chelating agents may compromise cytocompatibility. Er:YAG (erbium-doped yttrium aluminum garnet) laser conditioning offers an alternative approach with minimal chemical residue, and laser tip geometry may further influence biological outcomes. This study evaluated the cytocompatibility of Er:YAG laser dentin conditioning using End firing sapphire and side-firing tips by assessing human dental pulp – derived cell viability. Methods: Thirty-six single-rooted human teeth were decoronated and instrumented to ProTaper F4. Specimens were randomly allocated to four groups (n = 9): untreated control, Er:YAG laser with sapphire tip, Er:YAG laser with side-firing tip, and 17% EDTA. Following dentin conditioning, roots were longitudinally split to expose the canal wall, and human dental pulp–derived cells (1 × 104 cells/specimen) were seeded onto the treated surfaces. Cell viability, as an indirect indicator of cytocompatibility, was assessed using the MTT assay at Days 1, 4, and 7. Data were analyzed using one-way analysis of variance with Bonferroni post hoc tests (α = 0.05). Results: At all time points, the side-firing Er:YAG group demonstrated the highest cell viability, followed by the End firing sapphire-tip laser group. EDTA-conditioned dentin showed significantly lower viability, while the control group maintained baseline viability. Differences between groups became more pronounced over time. Conclusions: Er:YAG laser dentin conditioning enhanced human dental pulp–derived cell viability compared with EDTA, with the side-firing tip yielding the most favorable results. Side-firing Er:YAG tips may therefore provide a more cytocompatible dentin surface for regenerative endodontic applications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Photobiomodulation, Photomedicine & Laser Surgery is the property of Mary Ann Liebert, Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=194489391
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1177/25785478261453221
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 437
    Subjects:
      – SubjectFull: Dentin
        Type: general
      – SubjectFull: Cytocompatibility
        Type: general
      – SubjectFull: Ethylenediaminetetraacetic acid
        Type: general
      – SubjectFull: Cell survival
        Type: general
      – SubjectFull: Dental pulp
        Type: general
      – SubjectFull: Endodontics
        Type: general
      – SubjectFull: YAG lasers
        Type: general
    Titles:
      – TitleFull: Influence of Er:YAG Laser Dentin Conditioning with End-Firing Versus Side-Firing Tips on Human Dental Pulp–Derived Cell Viability.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Bangarimath, Parvati S.
      – PersonEntity:
          Name:
            NameFull: Merwade, Seema
      – PersonEntity:
          Name:
            NameFull: Kumar N, Kiran
      – PersonEntity:
          Name:
            NameFull: Bhavikatti, Tasmiya M.
      – PersonEntity:
          Name:
            NameFull: Kumari, Vandana
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Text: Jul2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 15495418
          Numbering:
            – Type: volume
              Value: 44
            – Type: issue
              Value: 7
          Titles:
            – TitleFull: Photobiomodulation, Photomedicine & Laser Surgery
              Type: main
ResultId 1