Dual-Stage Crosslinking of Gelatin-Alginate Bioink Supplemented with Wharton's Jelly to Generate 3D Bioprinted Scaffolds for Wound Healing Application.
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| Title: | Dual-Stage Crosslinking of Gelatin-Alginate Bioink Supplemented with Wharton's Jelly to Generate 3D Bioprinted Scaffolds for Wound Healing Application. |
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| Authors: | Phan, Nghia Thi Hieu1,2,3 (AUTHOR), Nguyen, Nho Thuan1,2,3 (AUTHOR), Tran, Ha Le Bao1,2,3 (AUTHOR), Nguyen, My Thi Ngoc1,2,3 (AUTHOR) ntnmy@hcmus.edu.vn |
| Source: | Polymers (20734360). Jun2026, Vol. 18 Issue 11, p1331. 20p. |
| Subjects: | Crosslinking (Polymerization), Wound healing, Biomimetic materials, Extracellular matrix, Bioprinting, Burns & scalds |
| Abstract: | Incorporation of extracellular matrix (ECM) components into bioinks can enhance biological functionality but often compromises print fidelity and structural stability. This study developed a dual-stage calcium chloride (CaCl2) crosslinking strategy to incorporate Wharton's jelly-derived ECM (WJ-ECM) into a gelatin-alginate bioink for bioprinted scaffold fabrication. A baseline formulation (BGA) and a WJ-ECM-supplemented formulation (BGAE, 1 mg/mL) were pre-crosslinked with 14 mM CaCl2 prior to extrusion, followed by secondary crosslinking in 0.5 M CaCl2 post-printing. Both formulations exhibited comparable viscosity (20–180 kcP) and high print fidelity (Pr = 0.99 ± 0.01 for BGA; 0.95 ± 0.01 for BGAE), with scaffolds displaying well-defined architecture and over 84% of pores within the target range (160–270 µm). FTIR analysis confirmed WJ-ECM incorporation without detectable alteration of the primary matrix structure. Both scaffolds were non-cytotoxic and supported fibroblast viability; BGAE constructs showed greater cell coverage over 14 days when surface-seeded and more stable fluorescence intensity through 28 days when encapsulated. In a murine thermal burn model, BGAE-treated wounds demonstrated more advanced re-epithelialization and more continuous epidermal coverage at day 14 compared to controls. These findings indicate that dual-stage crosslinking enables WJ-ECM integration while preserving printability, offering a practical platform for bioactive skin tissue engineering applications. [ABSTRACT FROM AUTHOR] |
| Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 194587595 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Dual-Stage Crosslinking of Gelatin-Alginate Bioink Supplemented with Wharton's Jelly to Generate 3D Bioprinted Scaffolds for Wound Healing Application. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Phan%2C+Nghia+Thi+Hieu%22">Phan, Nghia Thi Hieu</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nguyen%2C+Nho+Thuan%22">Nguyen, Nho Thuan</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tran%2C+Ha+Le+Bao%22">Tran, Ha Le Bao</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nguyen%2C+My+Thi+Ngoc%22">Nguyen, My Thi Ngoc</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> ntnmy@hcmus.edu.vn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Polymers+%2820734360%29%22">Polymers (20734360)</searchLink>. Jun2026, Vol. 18 Issue 11, p1331. 20p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Crosslinking+%28Polymerization%29%22">Crosslinking (Polymerization)</searchLink><br /><searchLink fieldCode="DE" term="%22Wound+healing%22">Wound healing</searchLink><br /><searchLink fieldCode="DE" term="%22Biomimetic+materials%22">Biomimetic materials</searchLink><br /><searchLink fieldCode="DE" term="%22Extracellular+matrix%22">Extracellular matrix</searchLink><br /><searchLink fieldCode="DE" term="%22Bioprinting%22">Bioprinting</searchLink><br /><searchLink fieldCode="DE" term="%22Burns+%26+scalds%22">Burns & scalds</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Incorporation of extracellular matrix (ECM) components into bioinks can enhance biological functionality but often compromises print fidelity and structural stability. This study developed a dual-stage calcium chloride (CaCl2) crosslinking strategy to incorporate Wharton's jelly-derived ECM (WJ-ECM) into a gelatin-alginate bioink for bioprinted scaffold fabrication. A baseline formulation (BGA) and a WJ-ECM-supplemented formulation (BGAE, 1 mg/mL) were pre-crosslinked with 14 mM CaCl2 prior to extrusion, followed by secondary crosslinking in 0.5 M CaCl2 post-printing. Both formulations exhibited comparable viscosity (20–180 kcP) and high print fidelity (Pr = 0.99 ± 0.01 for BGA; 0.95 ± 0.01 for BGAE), with scaffolds displaying well-defined architecture and over 84% of pores within the target range (160–270 µm). FTIR analysis confirmed WJ-ECM incorporation without detectable alteration of the primary matrix structure. Both scaffolds were non-cytotoxic and supported fibroblast viability; BGAE constructs showed greater cell coverage over 14 days when surface-seeded and more stable fluorescence intensity through 28 days when encapsulated. In a murine thermal burn model, BGAE-treated wounds demonstrated more advanced re-epithelialization and more continuous epidermal coverage at day 14 compared to controls. These findings indicate that dual-stage crosslinking enables WJ-ECM integration while preserving printability, offering a practical platform for bioactive skin tissue engineering applications. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/polym18111331 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 20 StartPage: 1331 Subjects: – SubjectFull: Crosslinking (Polymerization) Type: general – SubjectFull: Wound healing Type: general – SubjectFull: Biomimetic materials Type: general – SubjectFull: Extracellular matrix Type: general – SubjectFull: Bioprinting Type: general – SubjectFull: Burns & scalds Type: general Titles: – TitleFull: Dual-Stage Crosslinking of Gelatin-Alginate Bioink Supplemented with Wharton's Jelly to Generate 3D Bioprinted Scaffolds for Wound Healing Application. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Phan, Nghia Thi Hieu – PersonEntity: Name: NameFull: Nguyen, Nho Thuan – PersonEntity: Name: NameFull: Tran, Ha Le Bao – PersonEntity: Name: NameFull: Nguyen, My Thi Ngoc IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 20734360 Numbering: – Type: volume Value: 18 – Type: issue Value: 11 Titles: – TitleFull: Polymers (20734360) Type: main |
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