View in EDS

Engineering a high-efficiency expression system in Tremella fuciformis using novel promoters and optimized transformation conditions.

Saved in:
Bibliographic Details
Title: Engineering a high-efficiency expression system in Tremella fuciformis using novel promoters and optimized transformation conditions.
Authors: Hao, Mei1 (AUTHOR), Sheng, Li1 (AUTHOR), Mo, Cuiyuan1 (AUTHOR), Wei, Xuetuan1 (AUTHOR), Wang, Yuanyuan2 (AUTHOR) majestywyy@163.com, Ma, Aimin1 (AUTHOR) aiminma@mail.hzau.edu.cn
Source: World Journal of Microbiology & Biotechnology. Jun2026, Vol. 42 Issue 6, p1-12. 12p.
Subjects: Promoters (Genetics), Agrobacterium tumefaciens, Synthetic biology, Yeast fungi, Gene expression, Quantitative research, Fungi
Abstract: The development of a high-efficiency expression system is crucial for advancing the molecular breeding and synthetic biology of Tremella fuciformis. Here, the system is reported through the identification of potent endogenous promoters and the systematic optimization of an Agrobacterium tumefaciens-mediated transformation protocol. Key parameters for transformation were established, including optimal antibiotic concentrations (12.5 µg/mL hygromycin, 250 µg/mL cefotaxime) and the critical yeast-like cells (YLCs) density (OD600 0.6–0.8) for co-culture. In addition, scanning electron microscope (SEM) observation revealed that surface damage on YLCs treated with brown aluminum oxide facilitated T-DNA transfer from A. tumefaciens and resulted in a 2.25-fold increase in the number of transformants. Central to this system, gene_sp10042040.1 (P4), gene_sp10011850.1 (P5) and gene_sp10036670.1 (P7) were validated in transformed YLCs, where they drove the expression of the eGFP to levels 67.8-fold, 11.8-fold, and 7-fold higher than the control wild-type strain, respectively. Strikingly, these novel promoters outperformed the constitutive gpd promoter, with an increase in expression efficiency of up to 1476.74%. This integrated expression system, comprising an optimized transformation workflow and a set of superior endogenous promoters, provides a powerful platform for genetic manipulation and metabolic engineering in T. fuciformis. [ABSTRACT FROM AUTHOR]
Copyright of World Journal of Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 194750115
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Engineering a high-efficiency expression system in Tremella fuciformis using novel promoters and optimized transformation conditions.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Hao%2C+Mei%22">Hao, Mei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sheng%2C+Li%22">Sheng, Li</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mo%2C+Cuiyuan%22">Mo, Cuiyuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wei%2C+Xuetuan%22">Wei, Xuetuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yuanyuan%22">Wang, Yuanyuan</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> majestywyy@163.com</i><br /><searchLink fieldCode="AR" term="%22Ma%2C+Aimin%22">Ma, Aimin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> aiminma@mail.hzau.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22World+Journal+of+Microbiology+%26+Biotechnology%22">World Journal of Microbiology & Biotechnology</searchLink>. Jun2026, Vol. 42 Issue 6, p1-12. 12p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Promoters+%28Genetics%29%22">Promoters (Genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Agrobacterium+tumefaciens%22">Agrobacterium tumefaciens</searchLink><br /><searchLink fieldCode="DE" term="%22Synthetic+biology%22">Synthetic biology</searchLink><br /><searchLink fieldCode="DE" term="%22Yeast+fungi%22">Yeast fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Quantitative+research%22">Quantitative research</searchLink><br /><searchLink fieldCode="DE" term="%22Fungi%22">Fungi</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The development of a high-efficiency expression system is crucial for advancing the molecular breeding and synthetic biology of Tremella fuciformis. Here, the system is reported through the identification of potent endogenous promoters and the systematic optimization of an Agrobacterium tumefaciens-mediated transformation protocol. Key parameters for transformation were established, including optimal antibiotic concentrations (12.5 µg/mL hygromycin, 250 µg/mL cefotaxime) and the critical yeast-like cells (YLCs) density (OD600 0.6–0.8) for co-culture. In addition, scanning electron microscope (SEM) observation revealed that surface damage on YLCs treated with brown aluminum oxide facilitated T-DNA transfer from A. tumefaciens and resulted in a 2.25-fold increase in the number of transformants. Central to this system, gene_sp10042040.1 (P4), gene_sp10011850.1 (P5) and gene_sp10036670.1 (P7) were validated in transformed YLCs, where they drove the expression of the eGFP to levels 67.8-fold, 11.8-fold, and 7-fold higher than the control wild-type strain, respectively. Strikingly, these novel promoters outperformed the constitutive gpd promoter, with an increase in expression efficiency of up to 1476.74%. This integrated expression system, comprising an optimized transformation workflow and a set of superior endogenous promoters, provides a powerful platform for genetic manipulation and metabolic engineering in T. fuciformis. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of World Journal of Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=194750115
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s11274-026-05004-x
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 1
    Subjects:
      – SubjectFull: Promoters (Genetics)
        Type: general
      – SubjectFull: Agrobacterium tumefaciens
        Type: general
      – SubjectFull: Synthetic biology
        Type: general
      – SubjectFull: Yeast fungi
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Quantitative research
        Type: general
      – SubjectFull: Fungi
        Type: general
    Titles:
      – TitleFull: Engineering a high-efficiency expression system in Tremella fuciformis using novel promoters and optimized transformation conditions.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Hao, Mei
      – PersonEntity:
          Name:
            NameFull: Sheng, Li
      – PersonEntity:
          Name:
            NameFull: Mo, Cuiyuan
      – PersonEntity:
          Name:
            NameFull: Wei, Xuetuan
      – PersonEntity:
          Name:
            NameFull: Wang, Yuanyuan
      – PersonEntity:
          Name:
            NameFull: Ma, Aimin
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 06
              Text: Jun2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 09593993
          Numbering:
            – Type: volume
              Value: 42
            – Type: issue
              Value: 6
          Titles:
            – TitleFull: World Journal of Microbiology & Biotechnology
              Type: main
ResultId 1