Reduction of nitric oxide to HNO by sodium dithionite: kinetics and mechanism.
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| Title: | Reduction of nitric oxide to HNO by sodium dithionite: kinetics and mechanism. |
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| Authors: | Vargas, Paola1 (AUTHOR), Venâncio, Mateus F.2 (AUTHOR), Rocha, Willian R.3 (AUTHOR), Suarez, Sebastián A.4 (AUTHOR) sebastian.suarez@uam.es, Doctorovich, Fabio A.1 (AUTHOR) doctorovich@qi.fcen.uba.ar |
| Source: | Dalton Transactions: An International Journal of Inorganic Chemistry. 6/23/2026, Vol. 55 Issue 24, p9223-9233. 11p. |
| Subjects: | Sodium dithionite, Nitroxyl, Nitric oxide, Chemical kinetics, Oxidation-reduction reaction, Metalloproteins, Electron donors |
| Abstract: | Sodium dithionite is a widely used reductant in biochemical and industrial applications, yet its intrinsic instability and complex redox chemistry continue to pose challenges for mechanistic interpretation. One relatively underexplored aspect is its reactivity with nitric oxide (NO˙), a small redox-active signalling molecule. While dithionite is commonly employed to reduce metal centres in enzymes, its potential interaction with NO˙ may influence experimental outcomes in aqueous redox systems. Here, we show that under anaerobic, near-neutral aqueous conditions, dithionite reacts with NO˙ leading to the formation of azanone (HNO, nitroxyl), the one-electron-reduced and protonated congener of nitric oxide. Formation of HNO is supported by direct trapping experiments using Mn(III) porphyrins and by indirect detection of N2O, a characteristic product of HNO dimerization. These findings reveal a previously overlooked route for HNO generation in dithionite-containing systems and highlight potential artefacts in biochemical experiments involving NO˙ and strong reductants, particularly in studies probing thiol reactivity or metalloprotein function. [ABSTRACT FROM AUTHOR] |
| Copyright of Dalton Transactions: An International Journal of Inorganic Chemistry is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 194757149 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Reduction of nitric oxide to HNO by sodium dithionite: kinetics and mechanism. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Vargas%2C+Paola%22">Vargas, Paola</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Venâncio%2C+Mateus+F%2E%22">Venâncio, Mateus F.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rocha%2C+Willian+R%2E%22">Rocha, Willian R.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Suarez%2C+Sebastián+A%2E%22">Suarez, Sebastián A.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> sebastian.suarez@uam.es</i><br /><searchLink fieldCode="AR" term="%22Doctorovich%2C+Fabio+A%2E%22">Doctorovich, Fabio A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> doctorovich@qi.fcen.uba.ar</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Dalton+Transactions%3A+An+International+Journal+of+Inorganic+Chemistry%22">Dalton Transactions: An International Journal of Inorganic Chemistry</searchLink>. 6/23/2026, Vol. 55 Issue 24, p9223-9233. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Sodium+dithionite%22">Sodium dithionite</searchLink><br /><searchLink fieldCode="DE" term="%22Nitroxyl%22">Nitroxyl</searchLink><br /><searchLink fieldCode="DE" term="%22Nitric+oxide%22">Nitric oxide</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+kinetics%22">Chemical kinetics</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidation-reduction+reaction%22">Oxidation-reduction reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Metalloproteins%22">Metalloproteins</searchLink><br /><searchLink fieldCode="DE" term="%22Electron+donors%22">Electron donors</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Sodium dithionite is a widely used reductant in biochemical and industrial applications, yet its intrinsic instability and complex redox chemistry continue to pose challenges for mechanistic interpretation. One relatively underexplored aspect is its reactivity with nitric oxide (NO˙), a small redox-active signalling molecule. While dithionite is commonly employed to reduce metal centres in enzymes, its potential interaction with NO˙ may influence experimental outcomes in aqueous redox systems. Here, we show that under anaerobic, near-neutral aqueous conditions, dithionite reacts with NO˙ leading to the formation of azanone (HNO, nitroxyl), the one-electron-reduced and protonated congener of nitric oxide. Formation of HNO is supported by direct trapping experiments using Mn(III) porphyrins and by indirect detection of N2O, a characteristic product of HNO dimerization. These findings reveal a previously overlooked route for HNO generation in dithionite-containing systems and highlight potential artefacts in biochemical experiments involving NO˙ and strong reductants, particularly in studies probing thiol reactivity or metalloprotein function. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Dalton Transactions: An International Journal of Inorganic Chemistry is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1039/d6dt00559d Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 9223 Subjects: – SubjectFull: Sodium dithionite Type: general – SubjectFull: Nitroxyl Type: general – SubjectFull: Nitric oxide Type: general – SubjectFull: Chemical kinetics Type: general – SubjectFull: Oxidation-reduction reaction Type: general – SubjectFull: Metalloproteins Type: general – SubjectFull: Electron donors Type: general Titles: – TitleFull: Reduction of nitric oxide to HNO by sodium dithionite: kinetics and mechanism. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Vargas, Paola – PersonEntity: Name: NameFull: Venâncio, Mateus F. – PersonEntity: Name: NameFull: Rocha, Willian R. – PersonEntity: Name: NameFull: Suarez, Sebastián A. – PersonEntity: Name: NameFull: Doctorovich, Fabio A. IsPartOfRelationships: – BibEntity: Dates: – D: 23 M: 06 Text: 6/23/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 14779226 Numbering: – Type: volume Value: 55 – Type: issue Value: 24 Titles: – TitleFull: Dalton Transactions: An International Journal of Inorganic Chemistry Type: main |
| ResultId | 1 |
