Perspectives on Atomic Force Microscopy for Investigating Microbial Extracellular Electron Transfer.
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| Title: | Perspectives on Atomic Force Microscopy for Investigating Microbial Extracellular Electron Transfer. |
|---|---|
| Authors: | Tian, Xiaochun1 (AUTHOR), Ren, Chongyuan1,2 (AUTHOR), Xu, Yizi1,3 (AUTHOR), Bai, Rui1,2 (AUTHOR), Wu, Xuee3 (AUTHOR) xewu@xmu.edu.cn, Zhao, Feng1 (AUTHOR) fzhao@iue.ac.cn |
| Source: | Fuel Cells. Jun2026, Vol. 26 Issue 3, p1-6. 6p. |
| Subjects: | Atomic force microscopy, Bioelectrochemistry, Microorganisms, Microbiology, Nanobiotechnology, Microbial fuel cells |
| Abstract: | Extracellular electron transfer (EET) in electroactive microorganisms plays a critical role in the bioenergy conversion processes of bioelectrochemical systems. Atomic force microscopy (AFM) has emerged as a powerful tool for probing microbial ultrastructure and electrical properties, particularly for resolving conductive features such as bacterial nanowires at the nanoscale. However, real‐time in situ characterization of dynamic EET processes under physiologically relevant aqueous conditions remains technically challenging. This perspective summarizes recent advances in multimodal AFM techniques, with particular emphasis on electrical and electrochemical AFM approaches for investigating EET mechanisms at the single‐cell level. We further discuss the opportunities and limitations of aqueous AFM measurements and integrated AFM platforms that enable enhanced electron transfer detection with micro‐ to nanoscale spatial resolution and molecular sensitivity. These developments provide new opportunities for the direct visualization and quantitative analysis of EET processes in living microbial systems. Continued progress in AFM‐based methodologies will advance the mechanistic understanding of microbial electroactivity and support the development of high‐efficiency bioelectrochemical systems, including microbial fuel cells. [ABSTRACT FROM AUTHOR] |
| Copyright of Fuel Cells is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 194871470 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Perspectives on Atomic Force Microscopy for Investigating Microbial Extracellular Electron Transfer. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tian%2C+Xiaochun%22">Tian, Xiaochun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ren%2C+Chongyuan%22">Ren, Chongyuan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xu%2C+Yizi%22">Xu, Yizi</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bai%2C+Rui%22">Bai, Rui</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wu%2C+Xuee%22">Wu, Xuee</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> xewu@xmu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Zhao%2C+Feng%22">Zhao, Feng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> fzhao@iue.ac.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Fuel+Cells%22">Fuel Cells</searchLink>. Jun2026, Vol. 26 Issue 3, p1-6. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Atomic+force+microscopy%22">Atomic force microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Bioelectrochemistry%22">Bioelectrochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Microorganisms%22">Microorganisms</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22Nanobiotechnology%22">Nanobiotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+fuel+cells%22">Microbial fuel cells</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Extracellular electron transfer (EET) in electroactive microorganisms plays a critical role in the bioenergy conversion processes of bioelectrochemical systems. Atomic force microscopy (AFM) has emerged as a powerful tool for probing microbial ultrastructure and electrical properties, particularly for resolving conductive features such as bacterial nanowires at the nanoscale. However, real‐time in situ characterization of dynamic EET processes under physiologically relevant aqueous conditions remains technically challenging. This perspective summarizes recent advances in multimodal AFM techniques, with particular emphasis on electrical and electrochemical AFM approaches for investigating EET mechanisms at the single‐cell level. We further discuss the opportunities and limitations of aqueous AFM measurements and integrated AFM platforms that enable enhanced electron transfer detection with micro‐ to nanoscale spatial resolution and molecular sensitivity. These developments provide new opportunities for the direct visualization and quantitative analysis of EET processes in living microbial systems. Continued progress in AFM‐based methodologies will advance the mechanistic understanding of microbial electroactivity and support the development of high‐efficiency bioelectrochemical systems, including microbial fuel cells. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Fuel Cells is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/fuce.70110 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 1 Subjects: – SubjectFull: Atomic force microscopy Type: general – SubjectFull: Bioelectrochemistry Type: general – SubjectFull: Microorganisms Type: general – SubjectFull: Microbiology Type: general – SubjectFull: Nanobiotechnology Type: general – SubjectFull: Microbial fuel cells Type: general Titles: – TitleFull: Perspectives on Atomic Force Microscopy for Investigating Microbial Extracellular Electron Transfer. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tian, Xiaochun – PersonEntity: Name: NameFull: Ren, Chongyuan – PersonEntity: Name: NameFull: Xu, Yizi – PersonEntity: Name: NameFull: Bai, Rui – PersonEntity: Name: NameFull: Wu, Xuee – PersonEntity: Name: NameFull: Zhao, Feng IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 16156846 Numbering: – Type: volume Value: 26 – Type: issue Value: 3 Titles: – TitleFull: Fuel Cells Type: main |
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