View in EDS

Nanopore-based Manipulation and Separation of Single-stranded DNA Molecules through Tuning pH Values.

Saved in:
Bibliographic Details
Title: Nanopore-based Manipulation and Separation of Single-stranded DNA Molecules through Tuning pH Values.
Authors: Zhou, Cheng-Kai1 (AUTHOR), Miao, Sheng-Wen1 (AUTHOR), Sun, Li-Zhen1 (AUTHOR) sunlizhen@zjut.edu.cn
Source: Chinese Journal of Polymer Science (Springer Science & Business Media B.V.). Jul2026, Vol. 44 Issue 7, p2361-2369. 9p.
Subjects: Single-stranded DNA, pH effect, Langevin equations, Nanopores, Proton transfer reactions, Electrostatic interaction, Nucleic acid separation
Abstract: The unique regulatory effect of pH on electrostatic interactions offers a powerful approach for manipulating and separating single-stranded DNA (ssDNA) molecules. In this study, we employ Langevin dynamics simulations to investigate the translocation dynamics of two ssDNAs, poly(dA) and poly(dT), through a silicon nitride nanopore under acidic conditions. The key distinction between the two chains is their different pH-dependent protonation. At low pH, the highly protonated adenine bases experience repulsive interactions from the similarly protonated nanopore surface and the retarding force from the external voltage, whereas neutral thymine bases do not. Consequently, compared to poly(dT), poly(dA) exhibits a lower capture probability and slower translocation speed under strongly acidic conditions. However, the difference in the translocation behaviors between the two chains gradually diminishes as the pH increases. Based on their distinct pH-dependent behaviors, poly(dA) and poly(dT) of identical length can be successfully separated through the translocation strategy at low pH, even when they are initially mixed on the same side of the nanopore. [ABSTRACT FROM AUTHOR]
Copyright of Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 195464565
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Nanopore-based Manipulation and Separation of Single-stranded DNA Molecules through Tuning pH Values.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Zhou%2C+Cheng-Kai%22">Zhou, Cheng-Kai</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Miao%2C+Sheng-Wen%22">Miao, Sheng-Wen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sun%2C+Li-Zhen%22">Sun, Li-Zhen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> sunlizhen@zjut.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Chinese+Journal+of+Polymer+Science+%28Springer+Science+%26+Business+Media+B%2EV%2E%29%22">Chinese Journal of Polymer Science (Springer Science & Business Media B.V.)</searchLink>. Jul2026, Vol. 44 Issue 7, p2361-2369. 9p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Single-stranded+DNA%22">Single-stranded DNA</searchLink><br /><searchLink fieldCode="DE" term="%22pH+effect%22">pH effect</searchLink><br /><searchLink fieldCode="DE" term="%22Langevin+equations%22">Langevin equations</searchLink><br /><searchLink fieldCode="DE" term="%22Nanopores%22">Nanopores</searchLink><br /><searchLink fieldCode="DE" term="%22Proton+transfer+reactions%22">Proton transfer reactions</searchLink><br /><searchLink fieldCode="DE" term="%22Electrostatic+interaction%22">Electrostatic interaction</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acid+separation%22">Nucleic acid separation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The unique regulatory effect of pH on electrostatic interactions offers a powerful approach for manipulating and separating single-stranded DNA (ssDNA) molecules. In this study, we employ Langevin dynamics simulations to investigate the translocation dynamics of two ssDNAs, poly(dA) and poly(dT), through a silicon nitride nanopore under acidic conditions. The key distinction between the two chains is their different pH-dependent protonation. At low pH, the highly protonated adenine bases experience repulsive interactions from the similarly protonated nanopore surface and the retarding force from the external voltage, whereas neutral thymine bases do not. Consequently, compared to poly(dT), poly(dA) exhibits a lower capture probability and slower translocation speed under strongly acidic conditions. However, the difference in the translocation behaviors between the two chains gradually diminishes as the pH increases. Based on their distinct pH-dependent behaviors, poly(dA) and poly(dT) of identical length can be successfully separated through the translocation strategy at low pH, even when they are initially mixed on the same side of the nanopore. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=195464565
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s10118-026-3640-7
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 9
        StartPage: 2361
    Subjects:
      – SubjectFull: Single-stranded DNA
        Type: general
      – SubjectFull: pH effect
        Type: general
      – SubjectFull: Langevin equations
        Type: general
      – SubjectFull: Nanopores
        Type: general
      – SubjectFull: Proton transfer reactions
        Type: general
      – SubjectFull: Electrostatic interaction
        Type: general
      – SubjectFull: Nucleic acid separation
        Type: general
    Titles:
      – TitleFull: Nanopore-based Manipulation and Separation of Single-stranded DNA Molecules through Tuning pH Values.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Zhou, Cheng-Kai
      – PersonEntity:
          Name:
            NameFull: Miao, Sheng-Wen
      – PersonEntity:
          Name:
            NameFull: Sun, Li-Zhen
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Text: Jul2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 02567679
          Numbering:
            – Type: volume
              Value: 44
            – Type: issue
              Value: 7
          Titles:
            – TitleFull: Chinese Journal of Polymer Science (Springer Science & Business Media B.V.)
              Type: main
ResultId 1