Bibliographic Details
| Title: |
Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin. |
| Authors: |
Syakhovich, Vitaly E.1, Saraswathi, N. T.2, Ruff, Marc2 ruff@igbmc.u-strasbg.fr, Bokut, Sergey B.1, Moras, Dino2 |
| Source: |
Acta Crystallographica: Section F (Wiley-Blackwell). Feb2006, Vol. 62 Issue 2, p106-109. 4p. 1 Color Photograph, 1 Black and White Photograph, 1 Chart, 1 Graph. |
| Subjects: |
Glycosylated hemoglobin, Glycoproteins, Hemoglobins, Optical diffraction, Crystallography, Crystallization |
| Abstract: |
Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 Å at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 Å, α = 90.00, β = 125.40, γ = 90.00°. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight ( VM) of 9.70 Å3 Da−1 and a solvent content of 49%. [ABSTRACT FROM AUTHOR] |
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| Database: |
Engineering Source |
