Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin.
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| Title: | Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin. |
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| Authors: | Syakhovich, Vitaly E.1, Saraswathi, N. T.2, Ruff, Marc2 ruff@igbmc.u-strasbg.fr, Bokut, Sergey B.1, Moras, Dino2 |
| Source: | Acta Crystallographica: Section F (Wiley-Blackwell). Feb2006, Vol. 62 Issue 2, p106-109. 4p. 1 Color Photograph, 1 Black and White Photograph, 1 Chart, 1 Graph. |
| Subjects: | Glycosylated hemoglobin, Glycoproteins, Hemoglobins, Optical diffraction, Crystallography, Crystallization |
| Abstract: | Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 Å at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 Å, α = 90.00, β = 125.40, γ = 90.00°. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight ( VM) of 9.70 Å3 Da−1 and a solvent content of 49%. [ABSTRACT FROM AUTHOR] |
| Copyright of Acta Crystallographica: Section F (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 19641836 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Syakhovich%2C+Vitaly+E%2E%22">Syakhovich, Vitaly E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Saraswathi%2C+N%2E+T%2E%22">Saraswathi, N. T.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ruff%2C+Marc%22">Ruff, Marc</searchLink><relatesTo>2</relatesTo><i> ruff@igbmc.u-strasbg.fr</i><br /><searchLink fieldCode="AR" term="%22Bokut%2C+Sergey+B%2E%22">Bokut, Sergey B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Moras%2C+Dino%22">Moras, Dino</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Acta+Crystallographica%3A+Section+F+%28Wiley-Blackwell%29%22">Acta Crystallographica: Section F (Wiley-Blackwell)</searchLink>. Feb2006, Vol. 62 Issue 2, p106-109. 4p. 1 Color Photograph, 1 Black and White Photograph, 1 Chart, 1 Graph. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Glycosylated+hemoglobin%22">Glycosylated hemoglobin</searchLink><br /><searchLink fieldCode="DE" term="%22Glycoproteins%22">Glycoproteins</searchLink><br /><searchLink fieldCode="DE" term="%22Hemoglobins%22">Hemoglobins</searchLink><br /><searchLink fieldCode="DE" term="%22Optical+diffraction%22">Optical diffraction</searchLink><br /><searchLink fieldCode="DE" term="%22Crystallography%22">Crystallography</searchLink><br /><searchLink fieldCode="DE" term="%22Crystallization%22">Crystallization</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 Å at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 Å, α = 90.00, β = 125.40, γ = 90.00°. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight ( VM) of 9.70 Å3 Da−1 and a solvent content of 49%. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Acta Crystallographica: Section F (Wiley-Blackwell) is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1107/S1744309105042764 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 106 Subjects: – SubjectFull: Glycosylated hemoglobin Type: general – SubjectFull: Glycoproteins Type: general – SubjectFull: Hemoglobins Type: general – SubjectFull: Optical diffraction Type: general – SubjectFull: Crystallography Type: general – SubjectFull: Crystallization Type: general Titles: – TitleFull: Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Syakhovich, Vitaly E. – PersonEntity: Name: NameFull: Saraswathi, N. T. – PersonEntity: Name: NameFull: Ruff, Marc – PersonEntity: Name: NameFull: Bokut, Sergey B. – PersonEntity: Name: NameFull: Moras, Dino IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2006 Type: published Y: 2006 Identifiers: – Type: issn-print Value: 17443091 Numbering: – Type: volume Value: 62 – Type: issue Value: 2 Titles: – TitleFull: Acta Crystallographica: Section F (Wiley-Blackwell) Type: main |
| ResultId | 1 |
