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Voltammetric measurement of electrically evoked dopamine levels in the striatum of the anesthetized Syrian hamster

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Title: Voltammetric measurement of electrically evoked dopamine levels in the striatum of the anesthetized Syrian hamster
Authors: Greco, Phillip G.1, Meisel, Robert L.2, Heidenreich, Byron A.1,3, Garris, Paul A.1,3 www.bio.ilstu.edu/garris
Source: Journal of Neuroscience Methods. Apr2006, Vol. 152 Issue 1/2, p55-64. 10p.
Subjects: Dopamine, Nucleus accumbens, Voltammetry, Golden hamster
Abstract: Abstract: Microdialysis measurements in the Syrian hamster clearly demonstrate a role for accumbal dopamine (DA) in female sexual behavior. However, large probe size and slow sampling rate prevent associating specific behaviors with DA changes in subregions of the heterogeneous nucleus accumbens. Fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM), which affords millisecond temporal resolution at a micron-sized probe, could address these important issues. Mostly used in other rodents, e.g. rats and mice, this technique has not been applied to hamsters. The goal of the present study was to establish the measurement of DA in the nucleus accumbens of the anesthetized male Syrian hamster using FSCV at a CFM. For comparison, DA was simultaneously measured in the caudate-putamen. Stimulation of the medial forebrain bundle was used to elicit DA. Electrically evoked DA levels in both striatal regions were sensitive to location of the stimulating electrode and CFM, stimulation frequency, inhibition of DA uptake by cocaine and DA autoreceptor blockade by raclopride. Regional differences were observed for DA release and uptake parameters, and the effects of cocaine. Taken together, these results establish the measurement of electrically evoked DA levels in the hamster striatum using FSCV at a CFM. [Copyright &y& Elsevier]
Copyright of Journal of Neuroscience Methods is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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  Data: Voltammetric measurement of electrically evoked dopamine levels in the striatum of the anesthetized Syrian hamster
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  Data: <searchLink fieldCode="AR" term="%22Greco%2C+Phillip+G%2E%22">Greco, Phillip G.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Meisel%2C+Robert+L%2E%22">Meisel, Robert L.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Heidenreich%2C+Byron+A%2E%22">Heidenreich, Byron A.</searchLink><relatesTo>1,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Garris%2C+Paul+A%2E%22">Garris, Paul A.</searchLink><relatesTo>1,3</relatesTo><i> www.bio.ilstu.edu/garris</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Neuroscience+Methods%22">Journal of Neuroscience Methods</searchLink>. Apr2006, Vol. 152 Issue 1/2, p55-64. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Dopamine%22">Dopamine</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleus+accumbens%22">Nucleus accumbens</searchLink><br /><searchLink fieldCode="DE" term="%22Voltammetry%22">Voltammetry</searchLink><br /><searchLink fieldCode="DE" term="%22Golden+hamster%22">Golden hamster</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Microdialysis measurements in the Syrian hamster clearly demonstrate a role for accumbal dopamine (DA) in female sexual behavior. However, large probe size and slow sampling rate prevent associating specific behaviors with DA changes in subregions of the heterogeneous nucleus accumbens. Fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM), which affords millisecond temporal resolution at a micron-sized probe, could address these important issues. Mostly used in other rodents, e.g. rats and mice, this technique has not been applied to hamsters. The goal of the present study was to establish the measurement of DA in the nucleus accumbens of the anesthetized male Syrian hamster using FSCV at a CFM. For comparison, DA was simultaneously measured in the caudate-putamen. Stimulation of the medial forebrain bundle was used to elicit DA. Electrically evoked DA levels in both striatal regions were sensitive to location of the stimulating electrode and CFM, stimulation frequency, inhibition of DA uptake by cocaine and DA autoreceptor blockade by raclopride. Regional differences were observed for DA release and uptake parameters, and the effects of cocaine. Taken together, these results establish the measurement of electrically evoked DA levels in the hamster striatum using FSCV at a CFM. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Journal of Neuroscience Methods is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.jneumeth.2005.08.009
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      – Code: eng
        Text: English
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      – SubjectFull: Voltammetry
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              Text: Apr2006
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