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Polymorphonuclear neutrophils and Escherichia coli proteases involved in proteolysis of casein during experimental E. coli mastitis

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Title: Polymorphonuclear neutrophils and Escherichia coli proteases involved in proteolysis of casein during experimental E. coli mastitis
Authors: Haddadi, K.1 kahina.haddadi@ensaia.inpl-nancy.fr, Prin-Mathieu, C.2, Moussaoui, F.1, Faure, G.C.2, Vangroenweghe, F.3, Burvenich, C.3, Le Roux, Y.1
Source: International Dairy Journal. Jun2006, Vol. 16 Issue 6, p639-647. 9p.
Subjects: Escherichia coli, Proteolysis, Caseins, Milking
Abstract: Abstract: An Escherichia coli mastitis model was used to characterize enzymes involved in bovine mammary tissue damage and proteolysis in milk. One-quarter each of four cows were inoculated with a suspension (104 cfu mL−1) of E. coli P4:O32. Blood and milk were collected before inoculation and for 216h afterwards. Intracellular elastase, collagenase and cathepsin activities were measured by flow cytometry of peripheral blood leukocytes and milk polymorphonuclear neutrophils (PMNs). Leukopenia occurred in peripheral blood 9h after infection, concomitant with an increase in somatic cell count in milk. Milk PMNs had lower activity of cathepsins and collagenase than peripheral blood PMNs. In parallel, milk samples were studied by zymography, and several proteases were detected in mastitic milk. These activities increased after infection, to reach a peak in 6h. However, total protease profiles and plasmin activities differed. It was concluded that proteases released by PMNs and E. coli contribute to proteolysis of casein during mastitis, as well as plasmin. [Copyright &y& Elsevier]
Copyright of International Dairy Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 20559009
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Polymorphonuclear neutrophils and Escherichia coli proteases involved in proteolysis of casein during experimental E. coli mastitis
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  Data: <searchLink fieldCode="AR" term="%22Haddadi%2C+K%2E%22">Haddadi, K.</searchLink><relatesTo>1</relatesTo><i> kahina.haddadi@ensaia.inpl-nancy.fr</i><br /><searchLink fieldCode="AR" term="%22Prin-Mathieu%2C+C%2E%22">Prin-Mathieu, C.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Moussaoui%2C+F%2E%22">Moussaoui, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Faure%2C+G%2EC%2E%22">Faure, G.C.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Vangroenweghe%2C+F%2E%22">Vangroenweghe, F.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Burvenich%2C+C%2E%22">Burvenich, C.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Le+Roux%2C+Y%2E%22">Le Roux, Y.</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22International+Dairy+Journal%22">International Dairy Journal</searchLink>. Jun2006, Vol. 16 Issue 6, p639-647. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Proteolysis%22">Proteolysis</searchLink><br /><searchLink fieldCode="DE" term="%22Caseins%22">Caseins</searchLink><br /><searchLink fieldCode="DE" term="%22Milking%22">Milking</searchLink>
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  Data: Abstract: An Escherichia coli mastitis model was used to characterize enzymes involved in bovine mammary tissue damage and proteolysis in milk. One-quarter each of four cows were inoculated with a suspension (104 cfu mL−1) of E. coli P4:O32. Blood and milk were collected before inoculation and for 216h afterwards. Intracellular elastase, collagenase and cathepsin activities were measured by flow cytometry of peripheral blood leukocytes and milk polymorphonuclear neutrophils (PMNs). Leukopenia occurred in peripheral blood 9h after infection, concomitant with an increase in somatic cell count in milk. Milk PMNs had lower activity of cathepsins and collagenase than peripheral blood PMNs. In parallel, milk samples were studied by zymography, and several proteases were detected in mastitic milk. These activities increased after infection, to reach a peak in 6h. However, total protease profiles and plasmin activities differed. It was concluded that proteases released by PMNs and E. coli contribute to proteolysis of casein during mastitis, as well as plasmin. [Copyright &y& Elsevier]
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  Data: <i>Copyright of International Dairy Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.idairyj.2005.09.014
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        Text: English
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        Type: general
      – SubjectFull: Proteolysis
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      – SubjectFull: Caseins
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              Text: Jun2006
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