The single subunit NADH dehydrogenase reduces generation of reactive oxygen species from complex I
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| Title: | The single subunit NADH dehydrogenase reduces generation of reactive oxygen species from complex I |
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| Authors: | Seo, Byoung Boo1, Marella, Mathieu1, Yagi, Takao yagi@scripps.edu, Matsuno-Yagi, Akemi1 |
| Source: | FEBS Letters. Nov2006, Vol. 580 Issue 26, p6105-6108. 4p. |
| Subjects: | Saccharomyces cerevisiae, Mitochondrial DNA, Neuroblastoma, Peroxidation |
| Abstract: | Abstract: Using rat dopaminergic and human neuroblastoma cell lines transduced with the NDI1 gene encoding the internal NADH dehydrogenase (Ndi1) from Saccharomyces cerevisiae, we investigated reactive oxygen species (ROS) generation caused by complex I inhibition. Incubation of non-transduced cells with rotenone elicited oxidative damage to mitochondrial DNA as well as lipid peroxidation. In contrast, oxidative stress was significantly decreased when the cells were transduced with NDI1. Furthermore, mitochondria from the NDI1-transduced cells showed a suppressed rate of ROS formation by the complex I inhibitors. We conclude that the Ndi1 enzyme is able to suppress ROS overproduction from defective complex I. [Copyright &y& Elsevier] |
| Copyright of FEBS Letters is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 22965241 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: The single subunit NADH dehydrogenase reduces generation of reactive oxygen species from complex I – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Seo%2C+Byoung+Boo%22">Seo, Byoung Boo</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Marella%2C+Mathieu%22">Marella, Mathieu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yagi%2C+Takao%22">Yagi, Takao</searchLink><i> yagi@scripps.edu</i><br /><searchLink fieldCode="AR" term="%22Matsuno-Yagi%2C+Akemi%22">Matsuno-Yagi, Akemi</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22FEBS+Letters%22">FEBS Letters</searchLink>. Nov2006, Vol. 580 Issue 26, p6105-6108. 4p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Saccharomyces+cerevisiae%22">Saccharomyces cerevisiae</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondrial+DNA%22">Mitochondrial DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Neuroblastoma%22">Neuroblastoma</searchLink><br /><searchLink fieldCode="DE" term="%22Peroxidation%22">Peroxidation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: Using rat dopaminergic and human neuroblastoma cell lines transduced with the NDI1 gene encoding the internal NADH dehydrogenase (Ndi1) from Saccharomyces cerevisiae, we investigated reactive oxygen species (ROS) generation caused by complex I inhibition. Incubation of non-transduced cells with rotenone elicited oxidative damage to mitochondrial DNA as well as lipid peroxidation. In contrast, oxidative stress was significantly decreased when the cells were transduced with NDI1. Furthermore, mitochondria from the NDI1-transduced cells showed a suppressed rate of ROS formation by the complex I inhibitors. We conclude that the Ndi1 enzyme is able to suppress ROS overproduction from defective complex I. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of FEBS Letters is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.febslet.2006.10.008 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 6105 Subjects: – SubjectFull: Saccharomyces cerevisiae Type: general – SubjectFull: Mitochondrial DNA Type: general – SubjectFull: Neuroblastoma Type: general – SubjectFull: Peroxidation Type: general Titles: – TitleFull: The single subunit NADH dehydrogenase reduces generation of reactive oxygen species from complex I Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Seo, Byoung Boo – PersonEntity: Name: NameFull: Marella, Mathieu – PersonEntity: Name: NameFull: Yagi, Takao – PersonEntity: Name: NameFull: Matsuno-Yagi, Akemi IsPartOfRelationships: – BibEntity: Dates: – D: 13 M: 11 Text: Nov2006 Type: published Y: 2006 Identifiers: – Type: issn-print Value: 00145793 Numbering: – Type: volume Value: 580 – Type: issue Value: 26 Titles: – TitleFull: FEBS Letters Type: main |
| ResultId | 1 |
