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An unconventional pathway for reduction of CO2 to methane in CO-grown Methanosarcina acetivorans revealed by proteomics.

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Title: An unconventional pathway for reduction of CO2 to methane in CO-grown Methanosarcina acetivorans revealed by proteomics.
Authors: Lessner, Daniel J.1, Lingyun Li2, Qingbo Li1,3, Rejtar, Tomas2, Andreev, Victor P.2, Reichlen, Matthew1, Hill, Kevin1, Moran, James J.4, Karger, Barry L.2 b.karger@neu.edu, Ferry, James G.1 jgf3@psu.edu
Source: Proceedings of the National Academy of Sciences of the United States of America. 11/21/2006, Vol. 103 Issue 47, p17921-17926. 6p. 2 Diagrams, 3 Charts, 1 Graph.
Subjects: Cobalt, Methane, Proteomics, Methanol, Acetates, Chemical reactions
Abstract: Methanosarcina acetivorans produces acetate, formate, and methane when cultured with CO as the growth substrate [Rother M, Metcalf WW (2004) Proc Natl Acad sci USA 101:16929–16934], which suggests novel features of CO metabolism. Here we present a genome-wide proteomic approach to identify and quantify proteins differentially abundant in response to growth on CO versus methanol or acetate. The results indicate that oxidation of CO to CO2 supplies electrons for reduction of CO2 to a methyl group by steps and enzymes of the pathway for CO2 reduction determined for other methane-producing species. However, proteomic and quantitative RT-PCR results suggest that reduction of the methyl group to methane involves novel methyltransferases and a coenzyme F420H2:heterodisulfide oxidoreductase system that generates a proton gradient for ATP synthesis not previously described for pathways reducing CO2 to methane. Biochemical assays support a role for the oxidoreductase, and transcriptional mapping identified an unusual operon structure encoding the oxidoreductase. The proteomic results further indicate that acetate is synthesized from the methyl group and CO by a reversal of initial steps in the pathway for conversion of acetate to methane that yields ATP by substrate level phosphorylation. The results indicate that M. acetivorans utilizes a pathway distinct from all known CO2 reduction pathways for methane formation that reflects an adaptation to the marine environment. Finally, the pathway supports the basis for a recently proposed primitive CO-dependent energy-conservation cycle that drove and directed the early evolution of life on Earth. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: An unconventional pathway for reduction of CO<subscript>2</subscript> to methane in CO-grown Methanosarcina acetivorans revealed by proteomics.
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  Data: <searchLink fieldCode="AR" term="%22Lessner%2C+Daniel+J%2E%22">Lessner, Daniel J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lingyun+Li%22">Lingyun Li</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Qingbo+Li%22">Qingbo Li</searchLink><relatesTo>1,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Rejtar%2C+Tomas%22">Rejtar, Tomas</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Andreev%2C+Victor+P%2E%22">Andreev, Victor P.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Reichlen%2C+Matthew%22">Reichlen, Matthew</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hill%2C+Kevin%22">Hill, Kevin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Moran%2C+James+J%2E%22">Moran, James J.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Karger%2C+Barry+L%2E%22">Karger, Barry L.</searchLink><relatesTo>2</relatesTo><i> b.karger@neu.edu</i><br /><searchLink fieldCode="AR" term="%22Ferry%2C+James+G%2E%22">Ferry, James G.</searchLink><relatesTo>1</relatesTo><i> jgf3@psu.edu</i>
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  Data: <searchLink fieldCode="DE" term="%22Cobalt%22">Cobalt</searchLink><br /><searchLink fieldCode="DE" term="%22Methane%22">Methane</searchLink><br /><searchLink fieldCode="DE" term="%22Proteomics%22">Proteomics</searchLink><br /><searchLink fieldCode="DE" term="%22Methanol%22">Methanol</searchLink><br /><searchLink fieldCode="DE" term="%22Acetates%22">Acetates</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+reactions%22">Chemical reactions</searchLink>
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  Data: Methanosarcina acetivorans produces acetate, formate, and methane when cultured with CO as the growth substrate [Rother M, Metcalf WW (2004) Proc Natl Acad sci USA 101:16929–16934], which suggests novel features of CO metabolism. Here we present a genome-wide proteomic approach to identify and quantify proteins differentially abundant in response to growth on CO versus methanol or acetate. The results indicate that oxidation of CO to CO2 supplies electrons for reduction of CO2 to a methyl group by steps and enzymes of the pathway for CO2 reduction determined for other methane-producing species. However, proteomic and quantitative RT-PCR results suggest that reduction of the methyl group to methane involves novel methyltransferases and a coenzyme F420H2:heterodisulfide oxidoreductase system that generates a proton gradient for ATP synthesis not previously described for pathways reducing CO2 to methane. Biochemical assays support a role for the oxidoreductase, and transcriptional mapping identified an unusual operon structure encoding the oxidoreductase. The proteomic results further indicate that acetate is synthesized from the methyl group and CO by a reversal of initial steps in the pathway for conversion of acetate to methane that yields ATP by substrate level phosphorylation. The results indicate that M. acetivorans utilizes a pathway distinct from all known CO2 reduction pathways for methane formation that reflects an adaptation to the marine environment. Finally, the pathway supports the basis for a recently proposed primitive CO-dependent energy-conservation cycle that drove and directed the early evolution of life on Earth. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1073/pnas.0608833103
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              Text: 11/21/2006
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