Sub Parts-Per-Million Mass Measurement Accuracy of Intact Proteins and Product Ions Achieved Using a Dual Electrospray Ionization Quadrupole Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Saved in:
| Title: | Sub Parts-Per-Million Mass Measurement Accuracy of Intact Proteins and Product Ions Achieved Using a Dual Electrospray Ionization Quadrupole Fourier Transform Ion Cyclotron Resonance Mass Spectrometer |
|---|---|
| Authors: | Williams, D. Keith1, Hawkridge, Adam M.1, Muddiman, David C. david_muddiman@ncsu.edu |
| Source: | Journal of the American Society for Mass Spectrometry. Jan2007, Vol. 18 Issue 1, p1-7. 7p. |
| Subjects: | Ion cyclotron resonance spectrometry, Mass spectrometers, Electrospray ionization mass spectrometry, Spectrometer calibration |
| Abstract: | High mass measurement accuracy (MMA) is demonstrated for intact proteins and subsequent collision-induced dissociation product ions using internal calibration. Internal calibration was accomplished using a dual electrospray ionization source coupled with a hybrid quadrupole Fourier transform ion cyclotron resonance (Q-FT-ICR) mass spectrometer. Initially, analyte ions generated via the first electrospray (ESI) emitter are isolated and dissociated in the external quadrupole. This event is followed by a simultaneous switch to the calibrant ion ESI emitter and a disablement of the isolation and activation of the external quadrupole such that a broad m/z range of calibrant ions are accumulated before injecting the analyte/calibrant ion mixture into the ICR cell. Two different internal calibrant solutions were utilized in these studies to evaluate this approach for the top-down characterization of melittin and ubiquitin. While external calibration of protein fragments resulted in absolute MMA greater than 16 ppm, internal standardization significantly improved upon the MMA of both the intact proteins and their products ions which ranged from −2.0 ppm to 1.1 ppm, with an average of −0.9 ppm. This method requires limited modification to ESI-FT-ICR mass spectrometers and is applicable for both positive and negative ionization modes. [Copyright &y& Elsevier] |
| Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 23604187 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Sub Parts-Per-Million Mass Measurement Accuracy of Intact Proteins and Product Ions Achieved Using a Dual Electrospray Ionization Quadrupole Fourier Transform Ion Cyclotron Resonance Mass Spectrometer – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Williams%2C+D%2E+Keith%22">Williams, D. Keith</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hawkridge%2C+Adam+M%2E%22">Hawkridge, Adam M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Muddiman%2C+David+C%2E%22">Muddiman, David C.</searchLink><i> david_muddiman@ncsu.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+the+American+Society+for+Mass+Spectrometry%22">Journal of the American Society for Mass Spectrometry</searchLink>. Jan2007, Vol. 18 Issue 1, p1-7. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Ion+cyclotron+resonance+spectrometry%22">Ion cyclotron resonance spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometers%22">Mass spectrometers</searchLink><br /><searchLink fieldCode="DE" term="%22Electrospray+ionization+mass+spectrometry%22">Electrospray ionization mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Spectrometer+calibration%22">Spectrometer calibration</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: High mass measurement accuracy (MMA) is demonstrated for intact proteins and subsequent collision-induced dissociation product ions using internal calibration. Internal calibration was accomplished using a dual electrospray ionization source coupled with a hybrid quadrupole Fourier transform ion cyclotron resonance (Q-FT-ICR) mass spectrometer. Initially, analyte ions generated via the first electrospray (ESI) emitter are isolated and dissociated in the external quadrupole. This event is followed by a simultaneous switch to the calibrant ion ESI emitter and a disablement of the isolation and activation of the external quadrupole such that a broad m/z range of calibrant ions are accumulated before injecting the analyte/calibrant ion mixture into the ICR cell. Two different internal calibrant solutions were utilized in these studies to evaluate this approach for the top-down characterization of melittin and ubiquitin. While external calibration of protein fragments resulted in absolute MMA greater than 16 ppm, internal standardization significantly improved upon the MMA of both the intact proteins and their products ions which ranged from −2.0 ppm to 1.1 ppm, with an average of −0.9 ppm. This method requires limited modification to ESI-FT-ICR mass spectrometers and is applicable for both positive and negative ionization modes. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=23604187 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jasms.2006.08.014 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 1 Subjects: – SubjectFull: Ion cyclotron resonance spectrometry Type: general – SubjectFull: Mass spectrometers Type: general – SubjectFull: Electrospray ionization mass spectrometry Type: general – SubjectFull: Spectrometer calibration Type: general Titles: – TitleFull: Sub Parts-Per-Million Mass Measurement Accuracy of Intact Proteins and Product Ions Achieved Using a Dual Electrospray Ionization Quadrupole Fourier Transform Ion Cyclotron Resonance Mass Spectrometer Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Williams, D. Keith – PersonEntity: Name: NameFull: Hawkridge, Adam M. – PersonEntity: Name: NameFull: Muddiman, David C. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 10440305 Numbering: – Type: volume Value: 18 – Type: issue Value: 1 Titles: – TitleFull: Journal of the American Society for Mass Spectrometry Type: main |
| ResultId | 1 |
