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Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection

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Title: Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection
Authors: Jeong, Ji-Seon1, Yoon, Hye-Ran2, Hong, Seon-Pyo1 seonhong@khu.ac.kr
Source: Journal of Chromatography A. Jan2007, Vol. 1140 Issue 1/2, p157-162. 6p.
Subjects: Galactosemia, Chromatographic analysis, Conductometric analysis, Galactose
Abstract: Abstract: We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3mM NaOH containing 1mM NaOAc, and 200mM NaOH was added for post-column reagent. The limit of detection (S/N=3) and limit of quantification (S/N=10) for galactose were 25ng/mL and 83ng/mL, respectively. Linear dynamic range was from 4.67mg/dL to 53.46mg/dL (r 2 =0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14–101.42%). [Copyright &y& Elsevier]
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Database: Engineering Source
Description
Abstract:Abstract: We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3mM NaOH containing 1mM NaOAc, and 200mM NaOH was added for post-column reagent. The limit of detection (S/N=3) and limit of quantification (S/N=10) for galactose were 25ng/mL and 83ng/mL, respectively. Linear dynamic range was from 4.67mg/dL to 53.46mg/dL (r 2 =0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14–101.42%). [Copyright &y& Elsevier]
ISSN:00219673
DOI:10.1016/j.chroma.2006.11.085