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Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility.

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Title: Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility.
Authors: Abascal, F. J.1 francisco.abascal@uca.es, Cosson, J.2, Fauvel, C.3
Source: Journal of Fish Biology. Feb2007, Vol. 70 Issue 2, p509-522. 14p. 1 Black and White Photograph, 1 Chart, 4 Graphs.
Subjects: European seabass, Sperm motility, Heavy metals, Sea basses, Spermatozoa
Abstract: Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (<50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating media contained up to 100 ppm of the metal salts. In contrast, Hg2+ modified the morphology of post-swimming spermatozoa at 0·4–1 ppm (sperm dilution rate 1:39) and completely arrested sperm motility at concentrations as low as 0·1 ppm (sperm dilution rate 1:2500). Assuming a covalent binding to sperm cells, this revealed a finite number of c. 10 million Hg2+ binding sites per spermatozoon. Complementary results using demembranated spermatozoa suggested that the main target of HgCl2 would be located in the plasma membrane and that HgCl2 would inhibit water channels, hence preventing sperm motility. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 23880474
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Abascal%2C+F%2E+J%2E%22&quot;&gt;Abascal, F. J.&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;i&gt; francisco.abascal@uca.es&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cosson%2C+J%2E%22&quot;&gt;Cosson, J.&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Fauvel%2C+C%2E%22&quot;&gt;Fauvel, C.&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Journal+of+Fish+Biology%22&quot;&gt;Journal of Fish Biology&lt;/searchLink&gt;. Feb2007, Vol. 70 Issue 2, p509-522. 14p. 1 Black and White Photograph, 1 Chart, 4 Graphs.
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22European+seabass%22&quot;&gt;European seabass&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Sperm+motility%22&quot;&gt;Sperm motility&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Heavy+metals%22&quot;&gt;Heavy metals&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Sea+basses%22&quot;&gt;Sea basses&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Spermatozoa%22&quot;&gt;Spermatozoa&lt;/searchLink&gt;
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  Data: Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (&lt;50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating media contained up to 100 ppm of the metal salts. In contrast, Hg2+ modified the morphology of post-swimming spermatozoa at 0&#183;4–1 ppm (sperm dilution rate 1:39) and completely arrested sperm motility at concentrations as low as 0&#183;1 ppm (sperm dilution rate 1:2500). Assuming a covalent binding to sperm cells, this revealed a finite number of c. 10 million Hg2+ binding sites per spermatozoon. Complementary results using demembranated spermatozoa suggested that the main target of HgCl2 would be located in the plasma membrane and that HgCl2 would inhibit water channels, hence preventing sperm motility. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: &lt;i&gt;Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/j.1095-8649.2007.01322.x
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 14
        StartPage: 509
    Subjects:
      – SubjectFull: European seabass
        Type: general
      – SubjectFull: Sperm motility
        Type: general
      – SubjectFull: Heavy metals
        Type: general
      – SubjectFull: Sea basses
        Type: general
      – SubjectFull: Spermatozoa
        Type: general
    Titles:
      – TitleFull: Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Abascal, F. J.
      – PersonEntity:
          Name:
            NameFull: Cosson, J.
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            NameFull: Fauvel, C.
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            – D: 01
              M: 02
              Text: Feb2007
              Type: published
              Y: 2007
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              Value: 00221112
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              Value: 70
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              Value: 2
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            – TitleFull: Journal of Fish Biology
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