Determination of phoxim residues in eggs by using high-performance liquid chromatography diode array detection after treatment of stocked housing facilities for the poultry red mite (Dermanyssus gallinae)
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| Title: | Determination of phoxim residues in eggs by using high-performance liquid chromatography diode array detection after treatment of stocked housing facilities for the poultry red mite (Dermanyssus gallinae) |
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| Authors: | Hamscher, G. gerd.hamscher@tiho-hannover.de, Prieß, B.1, Nau, H.1 |
| Source: | Analytica Chimica Acta. Mar2007, Vol. 586 Issue 1/2, p330-335. 6p. |
| Subjects: | Eggs, Chicken-mite, Antiparasitic agents, High performance liquid chromatography |
| Abstract: | Abstract: The poultry red mite Dermanyssus gallinae is the most important ectoparasite of poultry in several European countries. Phoxim is a well-known antiparasitic agent in wide use. Initial studies indicated that this compound could successfully be applied to eliminate D. gallinae in egg-laying birds and in henhouses by treating the cages and the equipment with it. In order to investigate whether phoxim residues are present in eggs from laying hens, we developed a selective and sensitive high-performance liquid chromatography method employing a simple water/acetonitrile gradient system. The amount of phoxim was determined by UV detection at 281nm, and the presence of the residue was confirmed by diode array detection. The eggs were homogenized for sample pretreatment and extracted with acetonitrile and partitioned with n-hexane. The acetonitrile extract was further purified with silica gel column chromatography. Recovery rates (performed at the 5–120μgkg−1 level) were in the range of 86.0–92.1% with relative standard deviations between 3.1% and 16.3%. Based on a signal to noise ratio of 3, the limit of detection of the assay was approximately 2μgkg−1. The day-to-day variation in the concentration of phoxim in four contaminated eggs (5.7–51.6μgkg−1) was generally less than 20%. The decision limit (CCα) and the detection capability (CCβ) were 62.0 and 68.7μgkg−1, respectively. The applicability of the method was demonstrated in eggs from three clinical trials and from a field study. In these investigations, all animals were kept in conventional battery cages. No sample was found containing more than the maximum residue level of 60μgkg−1 for phoxim in eggs as given in Annex I of Council Regulation (EEC) No. 2377/90. [Copyright &y& Elsevier] |
| Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Determination of phoxim residues in eggs by using high-performance liquid chromatography diode array detection after treatment of stocked housing facilities for the poultry red mite (Dermanyssus gallinae) – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Hamscher%2C+G%2E%22">Hamscher, G.</searchLink><i> gerd.hamscher@tiho-hannover.de</i><br /><searchLink fieldCode="AR" term="%22Prieß%2C+B%2E%22">Prieß, B.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nau%2C+H%2E%22">Nau, H.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Analytica+Chimica+Acta%22">Analytica Chimica Acta</searchLink>. Mar2007, Vol. 586 Issue 1/2, p330-335. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Eggs%22">Eggs</searchLink><br /><searchLink fieldCode="DE" term="%22Chicken-mite%22">Chicken-mite</searchLink><br /><searchLink fieldCode="DE" term="%22Antiparasitic+agents%22">Antiparasitic agents</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: The poultry red mite Dermanyssus gallinae is the most important ectoparasite of poultry in several European countries. Phoxim is a well-known antiparasitic agent in wide use. Initial studies indicated that this compound could successfully be applied to eliminate D. gallinae in egg-laying birds and in henhouses by treating the cages and the equipment with it. In order to investigate whether phoxim residues are present in eggs from laying hens, we developed a selective and sensitive high-performance liquid chromatography method employing a simple water/acetonitrile gradient system. The amount of phoxim was determined by UV detection at 281nm, and the presence of the residue was confirmed by diode array detection. The eggs were homogenized for sample pretreatment and extracted with acetonitrile and partitioned with n-hexane. The acetonitrile extract was further purified with silica gel column chromatography. Recovery rates (performed at the 5–120μgkg−1 level) were in the range of 86.0–92.1% with relative standard deviations between 3.1% and 16.3%. Based on a signal to noise ratio of 3, the limit of detection of the assay was approximately 2μgkg−1. The day-to-day variation in the concentration of phoxim in four contaminated eggs (5.7–51.6μgkg−1) was generally less than 20%. The decision limit (CCα) and the detection capability (CCβ) were 62.0 and 68.7μgkg−1, respectively. The applicability of the method was demonstrated in eggs from three clinical trials and from a field study. In these investigations, all animals were kept in conventional battery cages. No sample was found containing more than the maximum residue level of 60μgkg−1 for phoxim in eggs as given in Annex I of Council Regulation (EEC) No. 2377/90. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Analytica Chimica Acta is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.aca.2006.09.041 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 330 Subjects: – SubjectFull: Eggs Type: general – SubjectFull: Chicken-mite Type: general – SubjectFull: Antiparasitic agents Type: general – SubjectFull: High performance liquid chromatography Type: general Titles: – TitleFull: Determination of phoxim residues in eggs by using high-performance liquid chromatography diode array detection after treatment of stocked housing facilities for the poultry red mite (Dermanyssus gallinae) Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hamscher, G. – PersonEntity: Name: NameFull: Prieß, B. – PersonEntity: Name: NameFull: Nau, H. IsPartOfRelationships: – BibEntity: Dates: – D: 14 M: 03 Text: Mar2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 00032670 Numbering: – Type: volume Value: 586 – Type: issue Value: 1/2 Titles: – TitleFull: Analytica Chimica Acta Type: main |
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